Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs
The study aimed to assess the impact of dehydrated citrus pulp (DCP) on growth performance, fecal characteristics, fecal bacterial composition (based on 16S rRNA analysis), and fecal and serum metabolomic profiles in crossbred pigs. 80 finishing pigs Duroc × (Landrace × Large White) were fed either...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8733 https://www.nature.com/articles/s41598-023-44741-z |
| _version_ | 1855032844518162432 |
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| author | Belloumi, Dhekra Calvet, Salvador Roca, Marta Isabel Ferrer, Pablo Jiménez-Belenguer, Ana Cambra-López, María García-Rebollar, Paloma Climent, Eric Martínez-Blanch, Juan Tortajada, Marta Chenoll, Empar Bermejo, Almudena Cerisuelo, Alba |
| author_browse | Belloumi, Dhekra Bermejo, Almudena Calvet, Salvador Cambra-López, María Cerisuelo, Alba Chenoll, Empar Climent, Eric Ferrer, Pablo García-Rebollar, Paloma Jiménez-Belenguer, Ana Martínez-Blanch, Juan Roca, Marta Isabel Tortajada, Marta |
| author_facet | Belloumi, Dhekra Calvet, Salvador Roca, Marta Isabel Ferrer, Pablo Jiménez-Belenguer, Ana Cambra-López, María García-Rebollar, Paloma Climent, Eric Martínez-Blanch, Juan Tortajada, Marta Chenoll, Empar Bermejo, Almudena Cerisuelo, Alba |
| author_sort | Belloumi, Dhekra |
| collection | ReDivia |
| description | The study aimed to assess the impact of dehydrated citrus pulp (DCP) on growth performance, fecal characteristics, fecal bacterial composition (based on 16S rRNA analysis), and fecal and serum metabolomic profiles in crossbred pigs. 80 finishing pigs Duroc × (Landrace × Large White) were fed either a control diet (C) or a diet with 240 g/kg DCP (T) for six weeks. Including DCP in diets tended to decrease feed intake, increased (p < 0.05) the concentrations of acetic and heptanoic acids and decreased (p < 0.05) fecal butyric and branched-chain fatty acid concentrations in feces. Animals fed DCP exhibited a lower abundance of the genera Clostridium and Romboutsia, while Lachnospira significantly increased. Orthogonal partial least squares discriminant analysis plotted a clear separation of fecal and serum metabolites between groups. The main discriminant fecal metabolites were associated with bacterial protein fermentation and were downregulated in T-fed pigs. In serum, DCP supplementation upregulated metabolites related to protein and fatty acids metabolism. In conclusion, the addition of DCP as an environmentally friendly source of nutrients in pig diets, resulted in modifications of fecal bacterial composition, fermentation patterns, and overall pig metabolism, suggesting improvements in protein metabolism and gut health. |
| format | article |
| id | ReDivia8733 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia87332025-04-25T14:49:23Z Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs Belloumi, Dhekra Calvet, Salvador Roca, Marta Isabel Ferrer, Pablo Jiménez-Belenguer, Ana Cambra-López, María García-Rebollar, Paloma Climent, Eric Martínez-Blanch, Juan Tortajada, Marta Chenoll, Empar Bermejo, Almudena Cerisuelo, Alba Crossbred pigs Dehydrated citrus pulp Growth performance Q70 Processing of agricultural wastes L02 Animal feeding L51 Animal physiology - Nutrition L52 Animal physiology - Growth and development Biotechnology Fruit pulps Microbiota Byproducts Animal feeding The study aimed to assess the impact of dehydrated citrus pulp (DCP) on growth performance, fecal characteristics, fecal bacterial composition (based on 16S rRNA analysis), and fecal and serum metabolomic profiles in crossbred pigs. 80 finishing pigs Duroc × (Landrace × Large White) were fed either a control diet (C) or a diet with 240 g/kg DCP (T) for six weeks. Including DCP in diets tended to decrease feed intake, increased (p < 0.05) the concentrations of acetic and heptanoic acids and decreased (p < 0.05) fecal butyric and branched-chain fatty acid concentrations in feces. Animals fed DCP exhibited a lower abundance of the genera Clostridium and Romboutsia, while Lachnospira significantly increased. Orthogonal partial least squares discriminant analysis plotted a clear separation of fecal and serum metabolites between groups. The main discriminant fecal metabolites were associated with bacterial protein fermentation and were downregulated in T-fed pigs. In serum, DCP supplementation upregulated metabolites related to protein and fatty acids metabolism. In conclusion, the addition of DCP as an environmentally friendly source of nutrients in pig diets, resulted in modifications of fecal bacterial composition, fermentation patterns, and overall pig metabolism, suggesting improvements in protein metabolism and gut health. 2023-10-24T06:36:02Z 2023-10-24T06:36:02Z 2023 article publishedVersion Belloumi, D., Calvet, S., Roca, M. I., Ferrer, P., Jiménez-Belenguer, A., Cambra-López, M. et al. (2023). Effect of providing citrus pulp-integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs. Scientific Reports, 13(1), 17596. 2045-2322 (online) https://hdl.handle.net/20.500.11939/8733 10.1038/s41598-023-44741-z https://www.nature.com/articles/s41598-023-44741-z en This project was funded by the Spanish Ministry of Science and Innovation (AGL2014-56653 and RTI-2018- 095246-B-C22). Dhekra Belloumi is a recipient of the Santiago Grisolía PhD scholarship (GRISOLIAP/2020/023). info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-56653 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer Nature electronico |
| spellingShingle | Crossbred pigs Dehydrated citrus pulp Growth performance Q70 Processing of agricultural wastes L02 Animal feeding L51 Animal physiology - Nutrition L52 Animal physiology - Growth and development Biotechnology Fruit pulps Microbiota Byproducts Animal feeding Belloumi, Dhekra Calvet, Salvador Roca, Marta Isabel Ferrer, Pablo Jiménez-Belenguer, Ana Cambra-López, María García-Rebollar, Paloma Climent, Eric Martínez-Blanch, Juan Tortajada, Marta Chenoll, Empar Bermejo, Almudena Cerisuelo, Alba Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title | Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title_full | Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title_fullStr | Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title_full_unstemmed | Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title_short | Effect of providing citrus pulp‑integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| title_sort | effect of providing citrus pulp integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs |
| topic | Crossbred pigs Dehydrated citrus pulp Growth performance Q70 Processing of agricultural wastes L02 Animal feeding L51 Animal physiology - Nutrition L52 Animal physiology - Growth and development Biotechnology Fruit pulps Microbiota Byproducts Animal feeding |
| url | https://hdl.handle.net/20.500.11939/8733 https://www.nature.com/articles/s41598-023-44741-z |
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