Regulatory circuits involving bud dormancy factor PpeDAM6
DORMANCY-ASSOCIATED MADS-BOX (DAM) genes have recently emerged as key potential regulators of the dormancy cycle and climate adaptation in perennial species. Particularly, PpeDAM6 has been proposed to act as a major repressor of bud dormancy release and bud break in peach (Prunus persica). PpeDAM6 e...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2023
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| Acceso en línea: | https://hdl.handle.net/20.500.11939/8722 https://academic.oup.com/hr/article/doi/10.1038/s41438-021-00706-9/6491166?login=false |
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| author | Lloret, Alba Quesada-Traver, Carles Conejero, Ana Arbona, Vicent Gómez-Mena, Concepción Petri, Cesar Sánchez-Navarro, Jesús A. Zuriaga, Elena Leida, Carmen Badenes, María L. Ríos, Gabino |
| author_browse | Arbona, Vicent Badenes, María L. Conejero, Ana Gómez-Mena, Concepción Leida, Carmen Lloret, Alba Petri, Cesar Quesada-Traver, Carles Ríos, Gabino Sánchez-Navarro, Jesús A. Zuriaga, Elena |
| author_facet | Lloret, Alba Quesada-Traver, Carles Conejero, Ana Arbona, Vicent Gómez-Mena, Concepción Petri, Cesar Sánchez-Navarro, Jesús A. Zuriaga, Elena Leida, Carmen Badenes, María L. Ríos, Gabino |
| author_sort | Lloret, Alba |
| collection | ReDivia |
| description | DORMANCY-ASSOCIATED MADS-BOX (DAM) genes have recently emerged as key potential regulators of the dormancy cycle and climate adaptation in perennial species. Particularly, PpeDAM6 has been proposed to act as a major repressor of bud dormancy release and bud break in peach (Prunus persica). PpeDAM6 expression is downregulated concomitantly with the perception of a given genotype-dependent accumulation of winter chilling time, and the coincident enrichment in H3K27me3 chromatin modification at a specific genomic region. We have identified three peach BASIC PENTACYSTEINE PROTEINs (PpeBPCs) interacting with two GA-repeat motifs present in this H3K27me3-enriched region. Moreover, PpeBPC1 represses PpeDAM6 promoter activity by transient expression experiments. On the other hand, the heterologous overexpression of PpeDAM6 in European plum (Prunus domestica) alters plant vegetative growth, resulting in dwarf plants tending toward shoot meristem collapse. These alterations in vegetative growth of transgenic lines associate with impaired hormone homeostasis due to the modulation of genes involved in jasmonic acid, cytokinin, abscisic acid, and gibberellin pathways, and the downregulation of shoot meristem factors, specifically in transgenic leaf and apical tissues. The expression of many of these genes is also modified in flower buds of peach concomitantly with PpeDAM6 downregulation, which suggests a role of hormone homeostasis mechanisms in PpeDAM6-dependent maintenance of floral bud dormancy and growth repression. |
| format | Artículo |
| id | ReDivia8722 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia87222025-04-25T14:49:22Z Regulatory circuits involving bud dormancy factor PpeDAM6 Lloret, Alba Quesada-Traver, Carles Conejero, Ana Arbona, Vicent Gómez-Mena, Concepción Petri, Cesar Sánchez-Navarro, Jesús A. Zuriaga, Elena Leida, Carmen Badenes, María L. Ríos, Gabino Agricultural genetics F60 Plant physiology and biochemistry F62 Plant physiology - Growth and development U30 Research methods Flowering Plant physiology Transgenic plants Dormancy Bud dormancy Prunus domestica DORMANCY-ASSOCIATED MADS-BOX (DAM) genes have recently emerged as key potential regulators of the dormancy cycle and climate adaptation in perennial species. Particularly, PpeDAM6 has been proposed to act as a major repressor of bud dormancy release and bud break in peach (Prunus persica). PpeDAM6 expression is downregulated concomitantly with the perception of a given genotype-dependent accumulation of winter chilling time, and the coincident enrichment in H3K27me3 chromatin modification at a specific genomic region. We have identified three peach BASIC PENTACYSTEINE PROTEINs (PpeBPCs) interacting with two GA-repeat motifs present in this H3K27me3-enriched region. Moreover, PpeBPC1 represses PpeDAM6 promoter activity by transient expression experiments. On the other hand, the heterologous overexpression of PpeDAM6 in European plum (Prunus domestica) alters plant vegetative growth, resulting in dwarf plants tending toward shoot meristem collapse. These alterations in vegetative growth of transgenic lines associate with impaired hormone homeostasis due to the modulation of genes involved in jasmonic acid, cytokinin, abscisic acid, and gibberellin pathways, and the downregulation of shoot meristem factors, specifically in transgenic leaf and apical tissues. The expression of many of these genes is also modified in flower buds of peach concomitantly with PpeDAM6 downregulation, which suggests a role of hormone homeostasis mechanisms in PpeDAM6-dependent maintenance of floral bud dormancy and growth repression. 2023-10-02T07:20:33Z 2023-10-02T07:20:33Z 2021 article publishedVersion Lloret, A., Quesada-Traver, C., Conejero, A., Arbona, V., Gómez-Mena, C., Petri, C. et al. (2021). Regulatory circuits involving bud dormancy factor PpeDAM6. Horticulture Research, 8, 261. 2662-6810 (Print ISSN) 2052-7276 (Online ISSN) https://hdl.handle.net/20.500.11939/8722 10.1038/s41438-021-00706-9 https://academic.oup.com/hr/article/doi/10.1038/s41438-021-00706-9/6491166?login=false en This research was funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-FEDER (RTA2013-00026-C03-01, RTA2017-00011-C03-01). A. L. was funded by a fellowship from Ministerio de Ciencia (Spanish Government). C. Q.-T. was funded by a fellowship co-financed by the European Social Fund and the IVIA. info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/RTA2013-00026-C03-01/ES/Mejora sostenible de albaricoquero y melocotonero info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/RTA2017-00011-C03-01/ES/Mejora de albaricoquero y melocotonero en un contexto de agricultura sostenible y cambio climático Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Oxford University Press electronico |
| spellingShingle | Agricultural genetics F60 Plant physiology and biochemistry F62 Plant physiology - Growth and development U30 Research methods Flowering Plant physiology Transgenic plants Dormancy Bud dormancy Prunus domestica Lloret, Alba Quesada-Traver, Carles Conejero, Ana Arbona, Vicent Gómez-Mena, Concepción Petri, Cesar Sánchez-Navarro, Jesús A. Zuriaga, Elena Leida, Carmen Badenes, María L. Ríos, Gabino Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title | Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title_full | Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title_fullStr | Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title_full_unstemmed | Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title_short | Regulatory circuits involving bud dormancy factor PpeDAM6 |
| title_sort | regulatory circuits involving bud dormancy factor ppedam6 |
| topic | Agricultural genetics F60 Plant physiology and biochemistry F62 Plant physiology - Growth and development U30 Research methods Flowering Plant physiology Transgenic plants Dormancy Bud dormancy Prunus domestica |
| url | https://hdl.handle.net/20.500.11939/8722 https://academic.oup.com/hr/article/doi/10.1038/s41438-021-00706-9/6491166?login=false |
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