Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae
In this study, we identified Plasmopara-viticola-lesion-associated mononegaambi virus 3 (recently classified as Penicillimonavirus gammaplasmoparae), a fungi-associated mymonavirus, in grapevine plants showing an unusual upward curling symptomatology on the leaves and premature decline. Mymonavirida...
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| Format: | article |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8711 https://www.mdpi.com/2223-7747/12/18/3300/html |
| _version_ | 1855032840367898624 |
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| author | Morán, Félix Olmos, Antonio Candresse, Thierry Ruiz-García, Ana Belén |
| author_browse | Candresse, Thierry Morán, Félix Olmos, Antonio Ruiz-García, Ana Belén |
| author_facet | Morán, Félix Olmos, Antonio Candresse, Thierry Ruiz-García, Ana Belén |
| author_sort | Morán, Félix |
| collection | ReDivia |
| description | In this study, we identified Plasmopara-viticola-lesion-associated mononegaambi virus 3 (recently classified as Penicillimonavirus gammaplasmoparae), a fungi-associated mymonavirus, in grapevine plants showing an unusual upward curling symptomatology on the leaves and premature decline. Mymonaviridae is a family comprising nine genera of negative-sense single-stranded RNA viruses infecting filamentous fungi, although few of them have been associated with oomycetes, plants, and insects. Although the first mymonavirus genome description was reported a decade ago, the genome organization of several genera in the family, including the genus Penicillimonavirus, has remained unclear to date. We have determined the complete genome of P. gammaplasmoparae, which represents the first complete genomic sequence for this genus. Moreover, we provide strong evidence that P. gammaplasmoparae genome is bipartite and comprises two RNA molecules of around 6150 and 4560 nt. Our results indicate that the grapevine powdery mildew pathogen, Erysiphe necator, was also present in the analyzed plants and suggest P. gammaplasmoparae could be infecting this fungus. However, whether the fungus and/or the mycovirus are associated with the symptomatology that initially prompted these efforts remains to be determined. |
| format | article |
| id | ReDivia8711 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia87112025-04-25T14:49:20Z Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae Morán, Félix Olmos, Antonio Candresse, Thierry Ruiz-García, Ana Belén grapevine HTS mymonavirus Penicillimonavirus gammaplasmoparae Erysiphe necator H20 Plant diseases Plant protection In this study, we identified Plasmopara-viticola-lesion-associated mononegaambi virus 3 (recently classified as Penicillimonavirus gammaplasmoparae), a fungi-associated mymonavirus, in grapevine plants showing an unusual upward curling symptomatology on the leaves and premature decline. Mymonaviridae is a family comprising nine genera of negative-sense single-stranded RNA viruses infecting filamentous fungi, although few of them have been associated with oomycetes, plants, and insects. Although the first mymonavirus genome description was reported a decade ago, the genome organization of several genera in the family, including the genus Penicillimonavirus, has remained unclear to date. We have determined the complete genome of P. gammaplasmoparae, which represents the first complete genomic sequence for this genus. Moreover, we provide strong evidence that P. gammaplasmoparae genome is bipartite and comprises two RNA molecules of around 6150 and 4560 nt. Our results indicate that the grapevine powdery mildew pathogen, Erysiphe necator, was also present in the analyzed plants and suggest P. gammaplasmoparae could be infecting this fungus. However, whether the fungus and/or the mycovirus are associated with the symptomatology that initially prompted these efforts remains to be determined. 2023-09-21T07:02:03Z 2023-09-21T07:02:03Z 2023 article publishedVersion Morán, F., Olmos, A., Candresse, T., Ruiz-García, A. B. Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae. Plants 2023, 12, 3300. 2223-7747 https://hdl.handle.net/20.500.11939/8711 10.3390/plants12183300 https://www.mdpi.com/2223-7747/12/18/3300/html en_US info:eu-repo/grantAgreement/EC/H2020/734736/EU/Virus free Fruit Nurseries/VirFree info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTA-2014-00061-C03-01/ES/Prevención y control de virosis emergentes de vid en España openAccess MDPI electronico |
| spellingShingle | grapevine HTS mymonavirus Penicillimonavirus gammaplasmoparae Erysiphe necator H20 Plant diseases Plant protection Morán, Félix Olmos, Antonio Candresse, Thierry Ruiz-García, Ana Belén Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title | Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title_full | Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title_fullStr | Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title_full_unstemmed | Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title_short | Complete Genome Characterization of Penicillimonavirus gammaplasmoparae, a Bipartite Member of the Family Mymonaviridae |
| title_sort | complete genome characterization of penicillimonavirus gammaplasmoparae a bipartite member of the family mymonaviridae |
| topic | grapevine HTS mymonavirus Penicillimonavirus gammaplasmoparae Erysiphe necator H20 Plant diseases Plant protection |
| url | https://hdl.handle.net/20.500.11939/8711 https://www.mdpi.com/2223-7747/12/18/3300/html |
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