Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles
Rabbit is a reflexively ovulating species. Accordingly, in the practice of artificial insemination (AI) ovulation must be induced via exogenous GnRH (Gonadotropin-Releasing Hormone) administration, which may be performed intramuscularly, subcutaneously, or intravaginally. Unfortunately, the bioavail...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8679 https://www.mdpi.com/2076-2615/13/10/1628 |
| _version_ | 1855032834475950080 |
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| author | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Salvador Vicente, José |
| author_browse | Marco-Jiménez, Francisco Salvador Vicente, José Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Salvador Vicente, José |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | Rabbit is a reflexively ovulating species. Accordingly, in the practice of artificial insemination (AI) ovulation must be induced via exogenous GnRH (Gonadotropin-Releasing Hormone) administration, which may be performed intramuscularly, subcutaneously, or intravaginally. Unfortunately, the bioavailability of the GnRH analogue when added to the extender is lower due to the proteolytic activity in the seminal plasma and the poor permeability of the vaginal mucosa. The aim of the study was to refine the practice of AI practice in rabbits by replacing parenteral GnRH analogue administration (subcutaneous, intravenous, or intramuscular injection) with intravaginal application, while reducing its concentration in the diluent. Extenders containing the buserelin acetate in chitosan–dextran sulphate and chitosan–alginate nanoparticles were designed and 356 females were inseminated. Reproductive performance of females inseminated with the two experimental extenders, receiving 4 μg of buserelin acetate intravaginally per doe, was compared with that in the control group, the does of which were inseminated with the extender without the GnRH analogue and induced to ovulate with 1 μg of buserelin acetate administered intramuscularly. The entrapment efficiency of the chitosan–dextran sulphate complex was higher than that of chitosan–alginate. However, females inseminated with both systems showed similar reproductive performance. We conclude that both nanoencapsulation systems are an efficient way of intravaginal ovulation induction, allowing a reduction in the level of the GnRH analogue normally used in seminal doses from 15–25 μg to 4 μg. |
| format | article |
| id | ReDivia8679 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia86792025-04-25T14:49:16Z Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles Viudes-De-Castro, María P. Marco-Jiménez, Francisco Salvador Vicente, José Nanoencapsulation Dextran sulphate Rabbits Artificial insemination Chitosan Alginates Induced ovulation Reproductive performance Rabbit is a reflexively ovulating species. Accordingly, in the practice of artificial insemination (AI) ovulation must be induced via exogenous GnRH (Gonadotropin-Releasing Hormone) administration, which may be performed intramuscularly, subcutaneously, or intravaginally. Unfortunately, the bioavailability of the GnRH analogue when added to the extender is lower due to the proteolytic activity in the seminal plasma and the poor permeability of the vaginal mucosa. The aim of the study was to refine the practice of AI practice in rabbits by replacing parenteral GnRH analogue administration (subcutaneous, intravenous, or intramuscular injection) with intravaginal application, while reducing its concentration in the diluent. Extenders containing the buserelin acetate in chitosan–dextran sulphate and chitosan–alginate nanoparticles were designed and 356 females were inseminated. Reproductive performance of females inseminated with the two experimental extenders, receiving 4 μg of buserelin acetate intravaginally per doe, was compared with that in the control group, the does of which were inseminated with the extender without the GnRH analogue and induced to ovulate with 1 μg of buserelin acetate administered intramuscularly. The entrapment efficiency of the chitosan–dextran sulphate complex was higher than that of chitosan–alginate. However, females inseminated with both systems showed similar reproductive performance. We conclude that both nanoencapsulation systems are an efficient way of intravaginal ovulation induction, allowing a reduction in the level of the GnRH analogue normally used in seminal doses from 15–25 μg to 4 μg. 2023-07-19T09:55:28Z 2023-07-19T09:55:28Z 2023 article publishedVersion Viudes-de-Castro, M. P., Marco-Jiménez, F. & Salvador-Vicente, J. S. (2023). Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles. Animals, 13(10), 1628. 2076-2615 https://hdl.handle.net/20.500.11939/8679 10.3390/ani13101628 https://www.mdpi.com/2076-2615/13/10/1628 en This research was funded through grant PID2021-127867OB-100 by MCIN/AEI/10.13039/ 501100011033 and by ERDF, “A way of making Europe”, and through grant 52201K by GVA-IVIA and the FEDER Operational Program of the Valencian Community, 2021–2027. info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVal info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2021-127867OB-100 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Nanoencapsulation Dextran sulphate Rabbits Artificial insemination Chitosan Alginates Induced ovulation Reproductive performance Viudes-De-Castro, María P. Marco-Jiménez, Francisco Salvador Vicente, José Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title | Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title_full | Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title_fullStr | Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title_full_unstemmed | Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title_short | Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles |
| title_sort | reproductive performance of female rabbits inseminated with extenders supplemented with gnrh analogue entrapped in chitosan based nanoparticles |
| topic | Nanoencapsulation Dextran sulphate Rabbits Artificial insemination Chitosan Alginates Induced ovulation Reproductive performance |
| url | https://hdl.handle.net/20.500.11939/8679 https://www.mdpi.com/2076-2615/13/10/1628 |
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