New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots
In the current climate change scenario, water stress is a serious threat to limit crop growth and yields. It is necessary to develop tolerant plants that cope with water stress and, for this purpose, tolerance mechanisms should be studied. NIBER® is a proven water stress- and salt-tolerant pepper hy...
| Main Authors: | , , , , , , , |
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| Format: | article |
| Language: | Inglés |
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Elsevier
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8664 https://www.sciencedirect.com/science/article/pii/S0168945223001486 |
| _version_ | 1855032831911133184 |
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| author | Padilla, Yaiza Gara Gisbert-Mullor, Ramón Bueso, Eduardo Zhang, Leilei Forment, Javier Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles |
| author_browse | Bueso, Eduardo Calatayud, Ángeles Forment, Javier Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Padilla, Yaiza Gara Zhang, Leilei |
| author_facet | Padilla, Yaiza Gara Gisbert-Mullor, Ramón Bueso, Eduardo Zhang, Leilei Forment, Javier Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles |
| author_sort | Padilla, Yaiza Gara |
| collection | ReDivia |
| description | In the current climate change scenario, water stress is a serious threat to limit crop growth and yields. It is necessary to develop tolerant plants that cope with water stress and, for this purpose, tolerance mechanisms should be studied. NIBER® is a proven water stress- and salt-tolerant pepper hybrid rootstock (Gisbert-Mullor et al., 2020; López-Serrano et al., 2020), but tolerance mechanisms remain unclear. In this experiment, NIBER® and A10 (a sensitive pepper accession (Penella et al., 2014)) response to short-term water stress at 5 h and 24 h was studied in terms of gene expression and metabolites content in roots. GO terms and gene expression analyses evidenced constitutive differences in the transcriptomic profile of NIBER® and A10, associated with detoxification systems of reactive oxygen species (ROS). Upon water stress, transcription factors like DREBs and MYC are upregulated and the levels of auxins, abscisic acid and jasmonic acid are increased in NIBER®. NIBER® tolerance mechanisms involve an increase in osmoprotectant sugars (i.e., trehalose, raffinose) and in antioxidants (spermidine), but lower contents of oxidized glutathione compared to A10, which indicates less oxidative damage. Moreover, the gene expression for aquaporins and chaperones is enhanced. These results show the main NIBER® strategies to overcome water stress. |
| format | article |
| id | ReDivia8664 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia86642025-04-25T14:49:14Z New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots Padilla, Yaiza Gara Gisbert-Mullor, Ramón Bueso, Eduardo Zhang, Leilei Forment, Javier Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles Osmolytes F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry P10 Water resources and management Q04 Food composition RNA sequence Capsicum annuum Drought Phytohormones Antioxidants Water stress Climate change Rootstocks Salt tolerance Gene expression Auxins Abscisic acid Jasmonic acid In the current climate change scenario, water stress is a serious threat to limit crop growth and yields. It is necessary to develop tolerant plants that cope with water stress and, for this purpose, tolerance mechanisms should be studied. NIBER® is a proven water stress- and salt-tolerant pepper hybrid rootstock (Gisbert-Mullor et al., 2020; López-Serrano et al., 2020), but tolerance mechanisms remain unclear. In this experiment, NIBER® and A10 (a sensitive pepper accession (Penella et al., 2014)) response to short-term water stress at 5 h and 24 h was studied in terms of gene expression and metabolites content in roots. GO terms and gene expression analyses evidenced constitutive differences in the transcriptomic profile of NIBER® and A10, associated with detoxification systems of reactive oxygen species (ROS). Upon water stress, transcription factors like DREBs and MYC are upregulated and the levels of auxins, abscisic acid and jasmonic acid are increased in NIBER®. NIBER® tolerance mechanisms involve an increase in osmoprotectant sugars (i.e., trehalose, raffinose) and in antioxidants (spermidine), but lower contents of oxidized glutathione compared to A10, which indicates less oxidative damage. Moreover, the gene expression for aquaporins and chaperones is enhanced. These results show the main NIBER® strategies to overcome water stress. 2023-06-22T08:42:38Z 2023-06-22T08:42:38Z 2023 article publishedVersion Padilla, Y. G., Gisbert-Mullor, R., Bueso, E., Zhang, L., Forment, J., Lucini, L., ... & Calatayud, Á. (2023). New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots. Plant Science, 333, 111731. 1873-2259 (online ISSN) 0168-9452 (Print ISSN) https://hdl.handle.net/20.500.11939/8664 10.1016/j.plantsci.2023.111731 https://www.sciencedirect.com/science/article/pii/S0168945223001486 en This work has been financed by Grant PID2020-118824RR-C21 funded by MCIN/AEI/10.13039/501100011033 and Grant PAID-11-21 funded by the Vicerrectorado de Investigación de la Universidad Politécnica de Valencia. Ramón Gisbert-Mullor is a beneficiary of a doctoral fellowship (FPU-MEFP (Spain)). Yaiza Gara Padilla is a beneficiary of grant PRE2018-086374, funded by MCIN/AEI/10.13039/ 501100011033 and, as appropriate, by “ESF Investing in your future”. info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-118824RR-C21/ES/IDENTIFICACION Y CARACTERIZACION DE LAS BASES MOLECULARES Y FISIOLOGICAS EN LA UTILIZACION DE PATRONES DE PIMIENTO TOLERANTES A TEMPERATURAS NO OPTIMAS Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Elsevier electronico |
| spellingShingle | Osmolytes F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry P10 Water resources and management Q04 Food composition RNA sequence Capsicum annuum Drought Phytohormones Antioxidants Water stress Climate change Rootstocks Salt tolerance Gene expression Auxins Abscisic acid Jasmonic acid Padilla, Yaiza Gara Gisbert-Mullor, Ramón Bueso, Eduardo Zhang, Leilei Forment, Javier Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title | New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title_full | New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title_fullStr | New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title_full_unstemmed | New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title_short | New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| title_sort | new insights into short term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots |
| topic | Osmolytes F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry P10 Water resources and management Q04 Food composition RNA sequence Capsicum annuum Drought Phytohormones Antioxidants Water stress Climate change Rootstocks Salt tolerance Gene expression Auxins Abscisic acid Jasmonic acid |
| url | https://hdl.handle.net/20.500.11939/8664 https://www.sciencedirect.com/science/article/pii/S0168945223001486 |
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