Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy
The nutritional diagnosis of crops is carried out through costly elemental analyses of different plant organs, particularly leaves, in the laboratory. However, visible and near-infrared (Vis-NIR) spectroscopy of unprocessed plant samples has a high potential as a faster, non-destructive, environment...
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8639 https://www.mdpi.com/2073-4395/13/4/1105 |
| _version_ | 1855032827821686784 |
|---|---|
| author | Acosta, Maylin Visconti, Fernando Quinones, Ana Blasco, José De-Paz, José M. |
| author_browse | Acosta, Maylin Blasco, José De-Paz, José M. Quinones, Ana Visconti, Fernando |
| author_facet | Acosta, Maylin Visconti, Fernando Quinones, Ana Blasco, José De-Paz, José M. |
| author_sort | Acosta, Maylin |
| collection | ReDivia |
| description | The nutritional diagnosis of crops is carried out through costly elemental analyses of different plant organs, particularly leaves, in the laboratory. However, visible and near-infrared (Vis-NIR) spectroscopy of unprocessed plant samples has a high potential as a faster, non-destructive, environmental-friendly alternative to elemental analyses. In this work, the potential of this technique to estimate the concentrations of macronutrients such as nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg), and micronutrients such as iron (Fe), manganese (Mn) and boron (B), in persimmon (Diospyros kaki L.) ‘Rojo Brillante’ leaves, has been investigated. Throughout the crop cycle variable rates of N and K were applied to obtain six nutritional status levels in persimmon trees in an experimental orchard. Then, leaves were systematically sampled throughout the cropping season from the different nutritional levels and spectral reflectance measurements were acquired in the 430–1040 nm wavelength range. The concentrations of nutrients were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) for P, K, Ca, Mg, Fe, Mn and B after microwave digestion, while the Kjeldahl method was used for N. Then, partial least squares regression (PLS-R) was used to model the concentrations of these nutrients from the reflectance measurements of the leaves. The model was calibrated using 75% of the samples while the remaining 25% were left as the independent test set for external validation. The results of the test set indicated an acceptable validation for most of the nutrients, with determination coefficients (R2) of 0.74 for N and P, 0.54 for K, 0.77 for Ca, 0.60 for Mg, 0.39 for Fe, 0.69 for Mn and 0.83 for B. These findings support the potential use of Vis-NIR spectrometric techniques as an alternative to conventional laboratory methods for the persimmon nutritional status diagnosis although more research is needed to know how the models developed one year perform in ensuing years. |
| format | article |
| id | ReDivia8639 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia86392025-04-25T14:49:12Z Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy Acosta, Maylin Visconti, Fernando Quinones, Ana Blasco, José De-Paz, José M. Nutritional diagnosis Non-destructive Foliar analysis Vis-NIR spectroscopy Rojo brillante Chemometric Analysis Non-destructive techniques F61 Plant physiology - Nutrition Q04 Food composition U30 Research methods Macronutrients Micronutrients NIR spectroscopy Diospyros kaki Chemical analysis (results) The nutritional diagnosis of crops is carried out through costly elemental analyses of different plant organs, particularly leaves, in the laboratory. However, visible and near-infrared (Vis-NIR) spectroscopy of unprocessed plant samples has a high potential as a faster, non-destructive, environmental-friendly alternative to elemental analyses. In this work, the potential of this technique to estimate the concentrations of macronutrients such as nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg), and micronutrients such as iron (Fe), manganese (Mn) and boron (B), in persimmon (Diospyros kaki L.) ‘Rojo Brillante’ leaves, has been investigated. Throughout the crop cycle variable rates of N and K were applied to obtain six nutritional status levels in persimmon trees in an experimental orchard. Then, leaves were systematically sampled throughout the cropping season from the different nutritional levels and spectral reflectance measurements were acquired in the 430–1040 nm wavelength range. The concentrations of nutrients were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) for P, K, Ca, Mg, Fe, Mn and B after microwave digestion, while the Kjeldahl method was used for N. Then, partial least squares regression (PLS-R) was used to model the concentrations of these nutrients from the reflectance measurements of the leaves. The model was calibrated using 75% of the samples while the remaining 25% were left as the independent test set for external validation. The results of the test set indicated an acceptable validation for most of the nutrients, with determination coefficients (R2) of 0.74 for N and P, 0.54 for K, 0.77 for Ca, 0.60 for Mg, 0.39 for Fe, 0.69 for Mn and 0.83 for B. These findings support the potential use of Vis-NIR spectrometric techniques as an alternative to conventional laboratory methods for the persimmon nutritional status diagnosis although more research is needed to know how the models developed one year perform in ensuing years. 2023-06-02T12:25:40Z 2023-06-02T12:25:40Z 2023 article publishedVersion Acosta, M., Visconti, F., Quiñones, A., Blasco, J., & de-Paz, J. M. (2023). Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv.‘Rojo Brillante’Leaves through Vis-NIR Reflectance Spectroscopy. Agronomy, 13(4), 1105. 2073-4395 (eISSN) https://hdl.handle.net/20.500.11939/8639 10.3390/agronomy13041105 https://www.mdpi.com/2073-4395/13/4/1105 en This work was co-funded by the Programa de Becas IFARHU-SENACYT, subprograma Becas excelencia-PNDR (Panamá, reference: 270-2021-020), the Instituto Valenciano de Investigaciones Agrarias (IVIA-GVA, SostE-SABio project: references 52204, 51920) and by the EU through the European Regional Development Fund (ERDF) from GVA 2021–2027. info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52204/ES/Tecnología inteligente para una agricultura digital, sostenible y precisa en la comunitat valenciana/AgrIntel·ligència-CV info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52203/ES/Sostenibilidad y economía circular como ejes de desarrollo del sector agrario valenciano: suelo, agua y biodiversidad/SostE-SABio Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Nutritional diagnosis Non-destructive Foliar analysis Vis-NIR spectroscopy Rojo brillante Chemometric Analysis Non-destructive techniques F61 Plant physiology - Nutrition Q04 Food composition U30 Research methods Macronutrients Micronutrients NIR spectroscopy Diospyros kaki Chemical analysis (results) Acosta, Maylin Visconti, Fernando Quinones, Ana Blasco, José De-Paz, José M. Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title | Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title_full | Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title_fullStr | Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title_full_unstemmed | Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title_short | Estimation of Macro and Micronutrients in Persimmon (Diospyros kaki L.) cv. ‘Rojo Brillante’ Leaves through Vis-NIR Reflectance Spectroscopy |
| title_sort | estimation of macro and micronutrients in persimmon diospyros kaki l cv rojo brillante leaves through vis nir reflectance spectroscopy |
| topic | Nutritional diagnosis Non-destructive Foliar analysis Vis-NIR spectroscopy Rojo brillante Chemometric Analysis Non-destructive techniques F61 Plant physiology - Nutrition Q04 Food composition U30 Research methods Macronutrients Micronutrients NIR spectroscopy Diospyros kaki Chemical analysis (results) |
| url | https://hdl.handle.net/20.500.11939/8639 https://www.mdpi.com/2073-4395/13/4/1105 |
| work_keys_str_mv | AT acostamaylin estimationofmacroandmicronutrientsinpersimmondiospyroskakilcvrojobrillanteleavesthroughvisnirreflectancespectroscopy AT viscontifernando estimationofmacroandmicronutrientsinpersimmondiospyroskakilcvrojobrillanteleavesthroughvisnirreflectancespectroscopy AT quinonesana estimationofmacroandmicronutrientsinpersimmondiospyroskakilcvrojobrillanteleavesthroughvisnirreflectancespectroscopy AT blascojose estimationofmacroandmicronutrientsinpersimmondiospyroskakilcvrojobrillanteleavesthroughvisnirreflectancespectroscopy AT depazjosem estimationofmacroandmicronutrientsinpersimmondiospyroskakilcvrojobrillanteleavesthroughvisnirreflectancespectroscopy |