Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees
Xylella fastidiosa represents a major threat to important crops worldwide including almond, citrus, grapevine, and olives. Nowadays, there are no efficient control measures for X. fastidiosa, and the use of preventive measures and host resistance represent the most practical disease management strat...
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| Format: | article |
| Language: | Inglés |
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Frontiers Media
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8620 https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full#supplementary-material |
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| author | Anguita-Maeso, Manuel Ares-Yebra, Aitana Haro, Carmen Román-Écija, Miguel Olivares-García, Concepción Costa, Joana Marco-Noales, Ester Ferrer, Amparo Navas-Cortés, Juan A. Landa, Blanca B. |
| author_browse | Anguita-Maeso, Manuel Ares-Yebra, Aitana Costa, Joana Ferrer, Amparo Haro, Carmen Landa, Blanca B. Marco-Noales, Ester Navas-Cortés, Juan A. Olivares-García, Concepción Román-Écija, Miguel |
| author_facet | Anguita-Maeso, Manuel Ares-Yebra, Aitana Haro, Carmen Román-Écija, Miguel Olivares-García, Concepción Costa, Joana Marco-Noales, Ester Ferrer, Amparo Navas-Cortés, Juan A. Landa, Blanca B. |
| author_sort | Anguita-Maeso, Manuel |
| collection | ReDivia |
| description | Xylella fastidiosa represents a major threat to important crops worldwide including almond, citrus, grapevine, and olives. Nowadays, there are no efficient control measures for X. fastidiosa, and the use of preventive measures and host resistance represent the most practical disease management strategies. Research on vessel-associated microorganisms is gaining special interest as an innate natural defense of plants to cope against infection by xylem-inhabiting pathogens. The objective of this research has been to characterize, by next-generation sequencing (NGS) analysis, the microbial communities residing in the xylem sap of almond trees affected by almond leaf scorch disease (ALSD) in a recent X. fastidiosa outbreak occurring in Alicante province, Spain. We also determined community composition changes and network associations occurring between xylem-inhabiting microbial communities and X. fastidiosa. For that, a total of 91 trees with or without ALSD symptoms were selected from a total of eight representative orchards located in five municipalities within the X. fastidiosa-demarcated area. X. fastidiosa infection in each tree was verified by quantitative polymerase chain reaction (qPCR) analysis, with 54% of the trees being tested X. fastidiosa-positive. Globally, Xylella (27.4%), Sphingomonas (13.9%), and Hymenobacter (12.7%) were the most abundant bacterial genera, whereas Diplodia (30.18%), a member of the family Didymellaceae (10.7%), and Aureobasidium (9.9%) were the most predominant fungal taxa. Furthermore, principal coordinate analysis (PCoA) of Bray–Curtis and weighted UniFrac distances differentiated almond xylem bacterial communities mainly according to X. fastidiosa infection, in contrast to fungal community structure that was not closely related to the presence of the pathogen. Similar results were obtained when X. fastidiosa reads were removed from the bacterial data set although the effect was less pronounced. Co-occurrence network analysis revealed negative associations among four amplicon sequence variants (ASVs) assigned to X. fastidiosa with different bacterial ASVs belonging to 1174-901-12, Abditibacterium, Sphingomonas, Methylobacterium–Methylorubrum, Modestobacter, Xylophilus, and a non-identified member of the family Solirubrobacteraceae. Determination of the close-fitting associations between xylem-inhabiting microorganisms and X. fastidiosa may help to reveal specific microbial players associated with the suppression of ALSD under high X. fastidiosa inoculum pressure. These identified microorganisms would be good candidates to be tested in planta, to produce almond plants more resilient to X. fastidiosa infection when inoculated by endotherapy, contributing to suppress ALSD. |
| format | article |
| id | ReDivia8620 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia86202025-04-25T14:49:11Z Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees Anguita-Maeso, Manuel Ares-Yebra, Aitana Haro, Carmen Román-Écija, Miguel Olivares-García, Concepción Costa, Joana Marco-Noales, Ester Ferrer, Amparo Navas-Cortés, Juan A. Landa, Blanca B. Almond leaf scorch Microbiome Network associations Quarantine pathogens Microbial composition Almond trees RT-PCR Endophytic bacterial H20 Plant diseases U40 Surveying methods U10 Mathematical and statistical methods Xylem Xylella fastidiosa Statistical analysis Bioinformatics Infection Xylella fastidiosa represents a major threat to important crops worldwide including almond, citrus, grapevine, and olives. Nowadays, there are no efficient control measures for X. fastidiosa, and the use of preventive measures and host resistance represent the most practical disease management strategies. Research on vessel-associated microorganisms is gaining special interest as an innate natural defense of plants to cope against infection by xylem-inhabiting pathogens. The objective of this research has been to characterize, by next-generation sequencing (NGS) analysis, the microbial communities residing in the xylem sap of almond trees affected by almond leaf scorch disease (ALSD) in a recent X. fastidiosa outbreak occurring in Alicante province, Spain. We also determined community composition changes and network associations occurring between xylem-inhabiting microbial communities and X. fastidiosa. For that, a total of 91 trees with or without ALSD symptoms were selected from a total of eight representative orchards located in five municipalities within the X. fastidiosa-demarcated area. X. fastidiosa infection in each tree was verified by quantitative polymerase chain reaction (qPCR) analysis, with 54% of the trees being tested X. fastidiosa-positive. Globally, Xylella (27.4%), Sphingomonas (13.9%), and Hymenobacter (12.7%) were the most abundant bacterial genera, whereas Diplodia (30.18%), a member of the family Didymellaceae (10.7%), and Aureobasidium (9.9%) were the most predominant fungal taxa. Furthermore, principal coordinate analysis (PCoA) of Bray–Curtis and weighted UniFrac distances differentiated almond xylem bacterial communities mainly according to X. fastidiosa infection, in contrast to fungal community structure that was not closely related to the presence of the pathogen. Similar results were obtained when X. fastidiosa reads were removed from the bacterial data set although the effect was less pronounced. Co-occurrence network analysis revealed negative associations among four amplicon sequence variants (ASVs) assigned to X. fastidiosa with different bacterial ASVs belonging to 1174-901-12, Abditibacterium, Sphingomonas, Methylobacterium–Methylorubrum, Modestobacter, Xylophilus, and a non-identified member of the family Solirubrobacteraceae. Determination of the close-fitting associations between xylem-inhabiting microorganisms and X. fastidiosa may help to reveal specific microbial players associated with the suppression of ALSD under high X. fastidiosa inoculum pressure. These identified microorganisms would be good candidates to be tested in planta, to produce almond plants more resilient to X. fastidiosa infection when inoculated by endotherapy, contributing to suppress ALSD. 2023-05-23T09:57:09Z 2023-05-23T09:57:09Z 2022 article publishedVersion Anguita-Maeso, M., Ares-Yebra, A., Haro, C., Román-Écija, M., Olivares-García, C., Costa, J. et al. (2022). Xylella fastidiosa infection reshapes microbial composition and network associations in the xylem of almond trees. Frontiers in Microbiology, 13, 866085. 1664-302X https://hdl.handle.net/20.500.11939/8620 10.3389/fmicb.2022.866085 https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full#supplementary-material en This research was funded by projects AGL2016-75606-R and PID2020-114917RB-I00 (Programa Estatal de I+D Orientado a los Retos de la Sociedad from Spanish Government, the Spanish State Research Agency and FEDER-EU), XF-ACTORS (X. fastidiosa Active Containment Through a Multidisciplinary Oriented Research Strategy; grant 727987) and EcoStack (Grant Agreement No. 773554) from the European Union’s Horizon 2020 Framework Research Program. AA-Y acknowledges the STM program granted by the COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology). Financial support of CYTED network Iber-Xyfas (Ref. 119RT0569) is acknowledged. MA-M is a recipient of a research fellowship BES-2017-082361 from the Spanish Ministry of Economy and Competitiveness and CH is a recipient of a Juan de la Cierva fellowship “Grant IJC2019-040423-I” from the Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033. info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/AGL2016-75606-R/EL MICROBIOMA DEL OLIVO Y SU PAPEL EN LA RESPUESTA DE LA PLANTA A LA VERTICILOSIS CAUSADA POR VERTICILLIUM DAHLIAE: FACTORES DETERMINANTES Y MODIFICADORES info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-114917RB-I00/DESCIFRANDO, MODELIZANDO Y CULTIVANDO EL MICROBIOMA XILEMATICO DEL OLIVO PARA INCREMENTAR SU RESILIENCIA A ENFERMEDADES VASCULARES info:eu-repo/grantAgreement/EC/H2020/727987/EU//XF-ACTORS info:eu-repo/grantAgreement/EC/H2020/773554/EU//EcoStack Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Frontiers Media electronico |
| spellingShingle | Almond leaf scorch Microbiome Network associations Quarantine pathogens Microbial composition Almond trees RT-PCR Endophytic bacterial H20 Plant diseases U40 Surveying methods U10 Mathematical and statistical methods Xylem Xylella fastidiosa Statistical analysis Bioinformatics Infection Anguita-Maeso, Manuel Ares-Yebra, Aitana Haro, Carmen Román-Écija, Miguel Olivares-García, Concepción Costa, Joana Marco-Noales, Ester Ferrer, Amparo Navas-Cortés, Juan A. Landa, Blanca B. Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title | Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title_full | Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title_fullStr | Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title_full_unstemmed | Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title_short | Xylella fastidiosa Infection Reshapes Microbial Composition and Network Associations in the Xylem of Almond Trees |
| title_sort | xylella fastidiosa infection reshapes microbial composition and network associations in the xylem of almond trees |
| topic | Almond leaf scorch Microbiome Network associations Quarantine pathogens Microbial composition Almond trees RT-PCR Endophytic bacterial H20 Plant diseases U40 Surveying methods U10 Mathematical and statistical methods Xylem Xylella fastidiosa Statistical analysis Bioinformatics Infection |
| url | https://hdl.handle.net/20.500.11939/8620 https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full https://www.frontiersin.org/articles/10.3389/fmicb.2022.866085/full#supplementary-material |
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