Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp.
Xanthomonas is an important genus of plant-pathogenic bacteria that affects agronomic and economically important crops, causing serious economic losses. In fact, several Xanthomonas species are considered regulated quarantine pests. Due to the lack of effective control measures to treat plant-pathog...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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American Society for Microbiology (ASM)
2023
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| Acceso en línea: | https://hdl.handle.net/20.500.11939/8619 https://journals.asm.org/doi/10.1128/spectrum.02960-22 |
| _version_ | 1855492525404454912 |
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| author | Domingo-Calap, María L. Bernabeu-Gimeno, Mireia Aure, Cristina M. Marco-Noales, Ester Domingo-Calap, Pilar |
| author_browse | Aure, Cristina M. Bernabeu-Gimeno, Mireia Domingo-Calap, María L. Domingo-Calap, Pilar Marco-Noales, Ester |
| author_facet | Domingo-Calap, María L. Bernabeu-Gimeno, Mireia Aure, Cristina M. Marco-Noales, Ester Domingo-Calap, Pilar |
| author_sort | Domingo-Calap, María L. |
| collection | ReDivia |
| description | Xanthomonas is an important genus of plant-pathogenic bacteria that affects agronomic and economically important crops, causing serious economic losses. In fact, several Xanthomonas species are considered regulated quarantine pests. Due to the lack of effective control measures to treat plant-pathogenic bacteria, innovative control tools are needed to carry out integrated disease management. In this regard, bacteriophages (phages), viruses of bacteria, constitute a promising biocontrol tool. In this work, we report the isolation and characterization of 11 novel Xanthomonas arboricola pv. juglandis phages belonging to different families and genera of the class Caudoviricetes. Infectivity matrix in more than 60 isolates of different xanthomonads and other phytopathogenic bacteria suggests that these phages are specific to the Xanthomonas genus, with different host ranges depending on the isolates tested. Interestingly, some of these phages showed relevant features to be used as biocontrol tools to combat pathogenic Xanthomonas spp. as important as X. oryzae or X. citri. *Importance: Phytopathogenic bacteria represent serious losses worldwide. The lack of current treatments has focused the spotlight on phages, viruses of bacteria, as very promising biocontrol tools. Phages are very specific and can help to control bacterial infections in crops, as is the case of xanthomonads-associated diseases. The discovery of new environmental phages with lytic capacity that can help to combat these pathogens is of special relevance, and it is necessary to implement phage isolation and characterization techniques to determine their host range and their genomic properties. The establishment of phage collections worldwide will allow their use as preventive, diagnostic, or therapeutic tools. Although there is still a long way to go, this work is a step forward in the implementation of new ecofriendly techniques to combat key pathogens in the field. |
| format | Artículo |
| id | ReDivia8619 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | American Society for Microbiology (ASM) |
| publisherStr | American Society for Microbiology (ASM) |
| record_format | dspace |
| spelling | ReDivia86192025-04-25T14:49:10Z Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. Domingo-Calap, María L. Bernabeu-Gimeno, Mireia Aure, Cristina M. Marco-Noales, Ester Domingo-Calap, Pilar Plant-pathogenic bacteria Genome sequencing H20 Plant diseases U30 Research methods Phages Biocontrol Xanthomonas Xanthomonas is an important genus of plant-pathogenic bacteria that affects agronomic and economically important crops, causing serious economic losses. In fact, several Xanthomonas species are considered regulated quarantine pests. Due to the lack of effective control measures to treat plant-pathogenic bacteria, innovative control tools are needed to carry out integrated disease management. In this regard, bacteriophages (phages), viruses of bacteria, constitute a promising biocontrol tool. In this work, we report the isolation and characterization of 11 novel Xanthomonas arboricola pv. juglandis phages belonging to different families and genera of the class Caudoviricetes. Infectivity matrix in more than 60 isolates of different xanthomonads and other phytopathogenic bacteria suggests that these phages are specific to the Xanthomonas genus, with different host ranges depending on the isolates tested. Interestingly, some of these phages showed relevant features to be used as biocontrol tools to combat pathogenic Xanthomonas spp. as important as X. oryzae or X. citri. *Importance: Phytopathogenic bacteria represent serious losses worldwide. The lack of current treatments has focused the spotlight on phages, viruses of bacteria, as very promising biocontrol tools. Phages are very specific and can help to control bacterial infections in crops, as is the case of xanthomonads-associated diseases. The discovery of new environmental phages with lytic capacity that can help to combat these pathogens is of special relevance, and it is necessary to implement phage isolation and characterization techniques to determine their host range and their genomic properties. The establishment of phage collections worldwide will allow their use as preventive, diagnostic, or therapeutic tools. Although there is still a long way to go, this work is a step forward in the implementation of new ecofriendly techniques to combat key pathogens in the field. 2023-05-23T09:56:55Z 2023-05-23T09:56:55Z 2022 article publishedVersion Domingo-Calap, M. L., Bernabéu-Gimeno, M., Aure, C. M., Marco-Noales, E. & Domingo-Calap, P. (2022). Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. Microbiology Spectrum, 10(6), e02960-22. 2165-0497 (Online ISSN) https://hdl.handle.net/20.500.11939/8619 10.1128/spectrum.02960-22 https://journals.asm.org/doi/10.1128/spectrum.02960-22 en This work was funded by project SEJIGENT/2021/014, funded by Conselleria d’Innovació, Universitats, Ciència I Societat Digital (Generalitat Valenciana), to P.D.-C. and grant number 727987 XF-ACTORS (Xylella fastidiosa Active Containment Through a Multidisciplinary-Oriented Research Strategy) from European Union’s Horizon 2020 Research and Innovation Program and IVIA/52202D, Sostenible, funded by Instituto Valenciano de Investigaciones Agrarias, Generalitat, Valenciana to E.M.-N. P.D.-C. was financially supported by a Ramón y Cajal contract RYC2019-028015-I funded by Ministerio de Ciencia e Innovación. info:eu-repo/grantAgreement/EC/H2020/727987/EU//XF-ACTORS info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/RYC2019-028015-I Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess American Society for Microbiology (ASM) electronico |
| spellingShingle | Plant-pathogenic bacteria Genome sequencing H20 Plant diseases U30 Research methods Phages Biocontrol Xanthomonas Domingo-Calap, María L. Bernabeu-Gimeno, Mireia Aure, Cristina M. Marco-Noales, Ester Domingo-Calap, Pilar Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title | Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title_full | Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title_fullStr | Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title_full_unstemmed | Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title_short | Comparative Analysis of Novel Lytic Phages for Biological Control of Phytopathogenic Xanthomonas spp. |
| title_sort | comparative analysis of novel lytic phages for biological control of phytopathogenic xanthomonas spp |
| topic | Plant-pathogenic bacteria Genome sequencing H20 Plant diseases U30 Research methods Phages Biocontrol Xanthomonas |
| url | https://hdl.handle.net/20.500.11939/8619 https://journals.asm.org/doi/10.1128/spectrum.02960-22 |
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