Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus
Polyembryony and male sterility (MS) are essential characters for citrus breeding. MS, coupled with parthenocarpy, allows for addressing the diversification of diploid seedless mandarin varieties, and nucleocytoplasmic MS is the most prevalent system. Polyembryony limits the use of seed parents in s...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8618 https://www.mdpi.com/2223-7747/12/7/1567 |
| _version_ | 1855492525224099840 |
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| author | Montalt, Rafael Cuenca, José Vives, María C. Mournet, Pierre Navarro, Luis Ollitrault, Patrick Aleza, Pablo |
| author_browse | Aleza, Pablo Cuenca, José Montalt, Rafael Mournet, Pierre Navarro, Luis Ollitrault, Patrick Vives, María C. |
| author_facet | Montalt, Rafael Cuenca, José Vives, María C. Mournet, Pierre Navarro, Luis Ollitrault, Patrick Aleza, Pablo |
| author_sort | Montalt, Rafael |
| collection | ReDivia |
| description | Polyembryony and male sterility (MS) are essential characters for citrus breeding. MS, coupled with parthenocarpy, allows for addressing the diversification of diploid seedless mandarin varieties, and nucleocytoplasmic MS is the most prevalent system. Polyembryony limits the use of seed parents in scion breeding programs, and the recovery of monoembryonic hybrids to be used as female parents is a crucial pre-breeding component. The objectives of this work were the identification of SNPs closely linked with the genes implied in these traits for marker-assisted selection. Genotyping by sequencing was used to genotype 61 diploid hybrids from an F1 progeny recovered from crossing ‘Kiyomi’ and ‘Murcott’ tangors. A total of 6444 segregating markers were identified and used to establish the two parental genetic maps. They consisted of 1374 and 697 markers encompassing 1416.287 and 1339.735 cM for ‘Kiyomi’ and ‘Murcott’, respectively. Phenotyping for MS and polyembryony was performed. The genotype–trait association study identified a genomic region on LG8 which was significantly associated with MS, and a genomic region on LG1 which was significantly associated with polyembryony. Annotation of the identified region for MS revealed 19 candidate genes. One SNP KASPar marker was developed and fully validated for each trait. |
| format | Artículo |
| id | ReDivia8618 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia86182025-04-25T14:49:10Z Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus Montalt, Rafael Cuenca, José Vives, María C. Mournet, Pierre Navarro, Luis Ollitrault, Patrick Aleza, Pablo Association study Marker-assisted selection Genotyping Phenotyping Murcott F30 Plant genetics and breeding U30 Research methods Q01 Food science and technology F63 Plant physiology - Reproduction Mandarins Pollen grains Apomixis Citrus Polyembryony Seedlessness Polyembryony and male sterility (MS) are essential characters for citrus breeding. MS, coupled with parthenocarpy, allows for addressing the diversification of diploid seedless mandarin varieties, and nucleocytoplasmic MS is the most prevalent system. Polyembryony limits the use of seed parents in scion breeding programs, and the recovery of monoembryonic hybrids to be used as female parents is a crucial pre-breeding component. The objectives of this work were the identification of SNPs closely linked with the genes implied in these traits for marker-assisted selection. Genotyping by sequencing was used to genotype 61 diploid hybrids from an F1 progeny recovered from crossing ‘Kiyomi’ and ‘Murcott’ tangors. A total of 6444 segregating markers were identified and used to establish the two parental genetic maps. They consisted of 1374 and 697 markers encompassing 1416.287 and 1339.735 cM for ‘Kiyomi’ and ‘Murcott’, respectively. Phenotyping for MS and polyembryony was performed. The genotype–trait association study identified a genomic region on LG8 which was significantly associated with MS, and a genomic region on LG1 which was significantly associated with polyembryony. Annotation of the identified region for MS revealed 19 candidate genes. One SNP KASPar marker was developed and fully validated for each trait. 2023-05-23T09:56:39Z 2023-05-23T09:56:39Z 2023 article publishedVersion Montalt, R., Cuenca, J., Vives, M. C., Mournet, P., Navarro, L., Ollitrault, P., & Aleza, P. (2023). Genotyping by sequencing for SNP-Based linkage analysis and the development of KASPar markers for male sterility and polyembryony in citrus. Plants, 12(7), 1567. 2223-7747 https://hdl.handle.net/20.500.11939/8618 10.3390/plants12071567 https://www.mdpi.com/2223-7747/12/7/1567 en 128115OR info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2021-128115OR-I00 This research was supported by project PID2021-128115OR-I00, funded by Ministry of Science and Innovation, MCIN/AEI/10.13039/501100011033/FEDER, UE Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Association study Marker-assisted selection Genotyping Phenotyping Murcott F30 Plant genetics and breeding U30 Research methods Q01 Food science and technology F63 Plant physiology - Reproduction Mandarins Pollen grains Apomixis Citrus Polyembryony Seedlessness Montalt, Rafael Cuenca, José Vives, María C. Mournet, Pierre Navarro, Luis Ollitrault, Patrick Aleza, Pablo Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title | Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title_full | Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title_fullStr | Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title_full_unstemmed | Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title_short | Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus |
| title_sort | genotyping by sequencing for snp based linkage analysis and the development of kaspar markers for male sterility and polyembryony in citrus |
| topic | Association study Marker-assisted selection Genotyping Phenotyping Murcott F30 Plant genetics and breeding U30 Research methods Q01 Food science and technology F63 Plant physiology - Reproduction Mandarins Pollen grains Apomixis Citrus Polyembryony Seedlessness |
| url | https://hdl.handle.net/20.500.11939/8618 https://www.mdpi.com/2223-7747/12/7/1567 |
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