Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance
Phytohormones play an important role in regulating the plant behavior to drought. In previous studies, NIBER® pepper rootstock showed tolerance to drought in terms of production and fruit quality compared to ungrafted plants. In this study, our hypothesis was that short-term exposure to water stress...
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.11939/8614 https://www.frontiersin.org/articles/10.3389/fpls.2023.1170021/full |
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| author | Padilla, Yaiza Gara Gisbert-Mullor, Ramón López-Galarza, Salvador Albacete, Alfonso Martínez-Melgarejo, Purificación A. Calatayud, Ángeles |
| author_browse | Albacete, Alfonso Calatayud, Ángeles Gisbert-Mullor, Ramón López-Galarza, Salvador Martínez-Melgarejo, Purificación A. Padilla, Yaiza Gara |
| author_facet | Padilla, Yaiza Gara Gisbert-Mullor, Ramón López-Galarza, Salvador Albacete, Alfonso Martínez-Melgarejo, Purificación A. Calatayud, Ángeles |
| author_sort | Padilla, Yaiza Gara |
| collection | ReDivia |
| description | Phytohormones play an important role in regulating the plant behavior to drought. In previous studies, NIBER® pepper rootstock showed tolerance to drought in terms of production and fruit quality compared to ungrafted plants. In this study, our hypothesis was that short-term exposure to water stress in young, grafted pepper plants would shed light on tolerance to drought in terms of modulation of the hormonal balance. To validate this hypothesis, fresh weight, water use efficiency (WUE) and the main hormone classes were analyzed in self-grafted pepper plants (variety onto variety, V/V) and variety grafted onto NIBER® (V/N) at 4, 24, and 48h after severe water stress was induced by PEG addition. After 48h, WUE in V/N was higher than in V/V, due to major stomata closure to maintain water retention in the leaves. This can be explained by the higher abscisic acid (ABA) levels observed in the leaves of V/N plants. Despite the interaction between ABA and the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), in relation to stomata closure is controversial, we observed an important increase of ACC at the end of the experiment in V/N plants coinciding with an important rise of the WUE and ABA. The maximum concentration of jasmonic acid and salicylic acid after 48h was found in the leaves of V/N, associated with their role in abiotic stress signaling and tolerance. Respect to auxins and cytokinins, the highest concentrations were linked to water stress and NIBER®, but this effect did not occur for gibberellins. These results show that hormone balance was affected by water stress and rootstock genotype, where NIBER® rootstock displayed a better ability to overcome short-term water stress. |
| format | Artículo |
| id | ReDivia8614 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia86142025-04-25T14:49:09Z Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance Padilla, Yaiza Gara Gisbert-Mullor, Ramón López-Galarza, Salvador Albacete, Alfonso Martínez-Melgarejo, Purificación A. Calatayud, Ángeles F30 Plant genetics and breeding F62 Plant physiology - Growth and development P10 Water resources and management U10 Mathematical and statistical methods Water stress Capsicum annuum Drought stress Leaves Phytohormones Roots Water use efficiency Cytokinins Gibberellins Abscisic acid Salicylic acids Jasmonic acid Phytohormones play an important role in regulating the plant behavior to drought. In previous studies, NIBER® pepper rootstock showed tolerance to drought in terms of production and fruit quality compared to ungrafted plants. In this study, our hypothesis was that short-term exposure to water stress in young, grafted pepper plants would shed light on tolerance to drought in terms of modulation of the hormonal balance. To validate this hypothesis, fresh weight, water use efficiency (WUE) and the main hormone classes were analyzed in self-grafted pepper plants (variety onto variety, V/V) and variety grafted onto NIBER® (V/N) at 4, 24, and 48h after severe water stress was induced by PEG addition. After 48h, WUE in V/N was higher than in V/V, due to major stomata closure to maintain water retention in the leaves. This can be explained by the higher abscisic acid (ABA) levels observed in the leaves of V/N plants. Despite the interaction between ABA and the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), in relation to stomata closure is controversial, we observed an important increase of ACC at the end of the experiment in V/N plants coinciding with an important rise of the WUE and ABA. The maximum concentration of jasmonic acid and salicylic acid after 48h was found in the leaves of V/N, associated with their role in abiotic stress signaling and tolerance. Respect to auxins and cytokinins, the highest concentrations were linked to water stress and NIBER®, but this effect did not occur for gibberellins. These results show that hormone balance was affected by water stress and rootstock genotype, where NIBER® rootstock displayed a better ability to overcome short-term water stress. 2023-05-11T07:29:54Z 2023-05-11T07:29:54Z 2023 article publishedVersion Padilla, Y. G., Gisbert-Mullor, R., Lopez-Galarza, S., Albacete, A., Martínez-Melgarejo, P. A. & Calatayud, A. (2023). Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance. Frontiers in Plant Science, 14, 1170021. 1664-462X https://hdl.handle.net/20.500.11939/8614 10.3389/fpls.2023.1170021 https://www.frontiersin.org/articles/10.3389/fpls.2023.1170021/full en This work has been financed by Grant PID2020-118824RR-C21 funded by MCIN/AEI/10.13039/501100011033. RG-M is a beneficiary of a doctoral fellowship (FPU-MEFP (Spain)). YP is a beneficiary of grant PRE2018-086374 funded by MCIN/AEI/ 10.13039/501100011033 and, as appropriate, by “ESF Investing in your future”. info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-118824RR-C21/ES/IDENTIFICACION Y CARACTERIZACION DE LAS BASES MOLECULARES Y FISIOLOGICAS EN LA UTILIZACION DE PATRONES DE PIMIENTO TOLERANTES A TEMPERATURAS NO OPTIMAS Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Frontiers Media electronico |
| spellingShingle | F30 Plant genetics and breeding F62 Plant physiology - Growth and development P10 Water resources and management U10 Mathematical and statistical methods Water stress Capsicum annuum Drought stress Leaves Phytohormones Roots Water use efficiency Cytokinins Gibberellins Abscisic acid Salicylic acids Jasmonic acid Padilla, Yaiza Gara Gisbert-Mullor, Ramón López-Galarza, Salvador Albacete, Alfonso Martínez-Melgarejo, Purificación A. Calatayud, Ángeles Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title | Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title_full | Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title_fullStr | Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title_full_unstemmed | Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title_short | Short-term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| title_sort | short term water stress responses of grafted pepper plants are associated with changes in the hormonal balance |
| topic | F30 Plant genetics and breeding F62 Plant physiology - Growth and development P10 Water resources and management U10 Mathematical and statistical methods Water stress Capsicum annuum Drought stress Leaves Phytohormones Roots Water use efficiency Cytokinins Gibberellins Abscisic acid Salicylic acids Jasmonic acid |
| url | https://hdl.handle.net/20.500.11939/8614 https://www.frontiersin.org/articles/10.3389/fpls.2023.1170021/full |
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