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Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

One of the problems with the refrigerated storage of spermatozoa in goats is the short shelf life. The study of the effect of the different storage conditions on the di erent sperm parameters could help to increase sperm survival. In this work, we studied the effect of temperature and sperm concentr...

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Main Authors: Sadeghi, Sara, Del-Gallego, Raquel, García-Colomer, Balma, Gómez, Ernesto A., Yániz, Jesús L., Gosálvez, Jaime, López-Fernández, Carmen, Silvestre, Miguel A.
Format: article
Language:Inglés
Published: MDPI 2023
Subjects:
Goat sperm
Liquid storage
DNA fragmentation
Mitochondrial activity
Murciano-Granadina
Semen quality
L10 Animal genetics and breeding
U10 Mathematical and statistical methods
L53 Animal physiology - Reproduction
Motility
Ai (artificial insemination)
Genetic improvement
Frozen storage
Shelf life
Online Access:https://hdl.handle.net/20.500.11939/8583
https://www.mdpi.com/2079-7737/9/9/300
Summary:One of the problems with the refrigerated storage of spermatozoa in goats is the short shelf life. The study of the effect of the different storage conditions on the di erent sperm parameters could help to increase sperm survival. In this work, we studied the effect of temperature and sperm concentration during refrigerated storage on sperm motility, mitochondrial membrane potential and DNA fragmentation. We observed that refrigerated storage of semen doses reduced the majority of sperm quality parameters, however, sperm DNA fragmentation was not a ected. Storage at 5ºC preserved higher sperm motility than at 17ºC. Moreover, the reduction of sperm concentration below 500x10(6) sperm/mL did not seem to improve the quality of spermatozoa.

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