Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater
Ralstonia solanacearum is a bacterial phytopathogen affecting staple crops, originally from tropical and subtropical areas, whose ability to survive in temperate environments is of concern under global warming. In this study, two R. solanacearum strains from either cold or warm habitats were stresse...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8540 https://www.mdpi.com/2076-2607/10/12/2503 |
| _version_ | 1855032808311881728 |
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| author | Álvarez, Belén López, María M. Biosca, Elena G. |
| author_browse | Biosca, Elena G. López, María M. Álvarez, Belén |
| author_facet | Álvarez, Belén López, María M. Biosca, Elena G. |
| author_sort | Álvarez, Belén |
| collection | ReDivia |
| description | Ralstonia solanacearum is a bacterial phytopathogen affecting staple crops, originally from tropical and subtropical areas, whose ability to survive in temperate environments is of concern under global warming. In this study, two R. solanacearum strains from either cold or warm habitats were stressed by simultaneous exposure to natural oligotrophy at low (4°C), temperate (14°C), or warm (24°C) temperatures in environmental water. At 4°C, the effect of temperature was higher than that of oligotrophy, since R. solanacearum went into a viable but non-culturable (VBNC) state, which proved to be dependent on water nutrient contents. Resuscitation was demonstrated in vitro and in planta. At 14°C and 24°C, the effect of oligotrophy was higher than that of temperature on R. solanacearum populations, displaying starvation-survival responses and morphological changes which were stronger at 24°C. In tomato plants, starved, cold-induced VBNC, and/or resuscitated cells maintained virulence. The strains behaved similarly regardless of their cold or warm areas of origin. This work firstly describes the natural nutrient availability of environmental water favoring R. solanacearum survival, adaptations, and resuscitation in conditions that can be found in natural settings. These findings will contribute to anticipate the ability of R. solanacearum to spread, establish, and induce disease in new geographical and climatic areas. |
| format | article |
| id | ReDivia8540 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia85402025-04-25T14:49:03Z Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater Álvarez, Belén López, María M. Biosca, Elena G. Bacterial wilt Environmental stress VBNC (viable but non-culturable state) Pathogenicity H20 Plant diseases U30 Research methods Global warming Ralstonia solanacearum Ralstonia solanacearum is a bacterial phytopathogen affecting staple crops, originally from tropical and subtropical areas, whose ability to survive in temperate environments is of concern under global warming. In this study, two R. solanacearum strains from either cold or warm habitats were stressed by simultaneous exposure to natural oligotrophy at low (4°C), temperate (14°C), or warm (24°C) temperatures in environmental water. At 4°C, the effect of temperature was higher than that of oligotrophy, since R. solanacearum went into a viable but non-culturable (VBNC) state, which proved to be dependent on water nutrient contents. Resuscitation was demonstrated in vitro and in planta. At 14°C and 24°C, the effect of oligotrophy was higher than that of temperature on R. solanacearum populations, displaying starvation-survival responses and morphological changes which were stronger at 24°C. In tomato plants, starved, cold-induced VBNC, and/or resuscitated cells maintained virulence. The strains behaved similarly regardless of their cold or warm areas of origin. This work firstly describes the natural nutrient availability of environmental water favoring R. solanacearum survival, adaptations, and resuscitation in conditions that can be found in natural settings. These findings will contribute to anticipate the ability of R. solanacearum to spread, establish, and induce disease in new geographical and climatic areas. 2023-03-03T08:57:31Z 2023-03-03T08:57:31Z 2022 article publishedVersion Álvarez, B., López, M. M., & Biosca, E. G. (2022). Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in Environmental Water. Microorganisms, 10(12), 2503. 2076-2607 https://hdl.handle.net/20.500.11939/8540 10.3390/microorganisms10122503 https://www.mdpi.com/2076-2607/10/12/2503 en This research was funded by the EU project QLK 3-CT-2000-01598, the Spanish project RTA2015-00087-C02 financed by MCIN/AEI/10.13039/501100011033, the Spanish Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), and “ERDF A way of making Europe”, as well as the Laboratory of Reference for Plant Pathogenic Bacteria of the Spanish Ministry of Agriculture (MAPA) at Instituto Valenciano de Investigaciones Agrarias (IVIA), the University of Valencia (UV) project UV-INV-AE 112-66196, and the University of Valencia-Research Support to BACPLANT group GIUV2015-219. Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Bacterial wilt Environmental stress VBNC (viable but non-culturable state) Pathogenicity H20 Plant diseases U30 Research methods Global warming Ralstonia solanacearum Álvarez, Belén López, María M. Biosca, Elena G. Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title | Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title_full | Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title_fullStr | Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title_full_unstemmed | Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title_short | Ralstonia solanacearum Facing Spread-Determining Climatic Temperatures, Sustained Starvation, and Naturally Induced Resuscitation of Viable but Non-Culturable Cells in EnvironmentalWater |
| title_sort | ralstonia solanacearum facing spread determining climatic temperatures sustained starvation and naturally induced resuscitation of viable but non culturable cells in environmentalwater |
| topic | Bacterial wilt Environmental stress VBNC (viable but non-culturable state) Pathogenicity H20 Plant diseases U30 Research methods Global warming Ralstonia solanacearum |
| url | https://hdl.handle.net/20.500.11939/8540 https://www.mdpi.com/2076-2607/10/12/2503 |
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