Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs)...
| Main Authors: | , , , , |
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| Format: | article |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8494 https://www.mdpi.com/2073-4409/12/1/49 |
| _version_ | 1855032800171786240 |
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| author | Ruiz-García, Ana B. Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry |
| author_browse | Candresse, Thierry Faure, Chantal Marais, Armelle Olmos, Antonio Ruiz-García, Ana B. |
| author_facet | Ruiz-García, Ana B. Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry |
| author_sort | Ruiz-García, Ana B. |
| collection | ReDivia |
| description | The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported. |
| format | article |
| id | ReDivia8494 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia84942025-04-25T14:49:02Z Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid Ruiz-García, Ana B. Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry High-throughput sequencing (HTS) RT-PCR Natural hosts Apple dimple fruit viroid Plum viroid I H20 Plant diseases U40 Surveying methods Pomegranates Figs The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported. 2023-01-23T12:21:23Z 2023-01-23T12:21:23Z 2023 article publishedVersion Ruiz-García, A. B., Olmos, A., Marais, A., Faure, C. & Candresse, T. (2023). Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid. Cells, 12(1), 49. 2073-4409 https://hdl.handle.net/20.500.11939/8494 10.3390/cells12010049 https://www.mdpi.com/2073-4409/12/1/49 en Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | High-throughput sequencing (HTS) RT-PCR Natural hosts Apple dimple fruit viroid Plum viroid I H20 Plant diseases U40 Surveying methods Pomegranates Figs Ruiz-García, Ana B. Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title_full | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title_fullStr | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title_full_unstemmed | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title_short | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
| title_sort | natural infection of pomegranate punica granatum by apple dimple fruit viroid |
| topic | High-throughput sequencing (HTS) RT-PCR Natural hosts Apple dimple fruit viroid Plum viroid I H20 Plant diseases U40 Surveying methods Pomegranates Figs |
| url | https://hdl.handle.net/20.500.11939/8494 https://www.mdpi.com/2073-4409/12/1/49 |
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