Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..)
Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site - Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain. RGA-specific primers were developed from th...
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
ISHS
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8468 https://www.actahort.org/books/717/717_22.htm |
| _version_ | 1855492496364142592 |
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| author | Soriano, J. M. Romero, Carlos Vilanova, S. Llácer, Gerardo Badenes, María L. |
| author2 | Romojaro, Félix |
| author_browse | Badenes, María L. Llácer, Gerardo Romero, Carlos Romojaro, Félix Soriano, J. M. Vilanova, S. |
| author_facet | Romojaro, Félix Soriano, J. M. Romero, Carlos Vilanova, S. Llácer, Gerardo Badenes, María L. |
| author_sort | Soriano, J. M. |
| collection | ReDivia |
| description | Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site - Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain. RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer. Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations. Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar ‘Lito’. |
| format | Objeto de conferencia |
| id | ReDivia8468 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | ISHS |
| publisherStr | ISHS |
| record_format | dspace |
| spelling | ReDivia84682025-04-25T14:53:57Z Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) Soriano, J. M. Romero, Carlos Vilanova, S. Llácer, Gerardo Badenes, María L. Romojaro, Félix NBS-LRR sequences RGAs F30 Plant genetics and breeding Q01 Food science and technology U30 Research methods Apricots DNA sequence Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site - Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain. RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer. Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations. Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar ‘Lito’. 2022-12-14T17:06:09Z 2022-12-14T17:06:09Z 2006 conferenceObject Soriano, J. M., Romero, C., Vilanova, S., Llacer, G. & Badenes, M. L. (2006). Cloning and mapping of resistance gene homologues in apricot (Prunus armeniaca l.). Acta Horticulturae. 717, 115-118. 978-90-66055-99-5 0567-7572 (print) 2406-6168 (electronic) http://hdl.handle.net/20.500.11939/8468 10.17660/ActaHortic.2006.717.22 https://www.actahort.org/books/717/717_22.htm en 2005-06 XIII International Symposium on Apricot Breeding and Culture Murcia, España Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess ISHS electronico |
| spellingShingle | NBS-LRR sequences RGAs F30 Plant genetics and breeding Q01 Food science and technology U30 Research methods Apricots DNA sequence Soriano, J. M. Romero, Carlos Vilanova, S. Llácer, Gerardo Badenes, María L. Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title | Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title_full | Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title_fullStr | Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title_full_unstemmed | Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title_short | Cloning and Mapping of Resistance Gene Homologues in Apricot (Prunus armeniaca L..) |
| title_sort | cloning and mapping of resistance gene homologues in apricot prunus armeniaca l |
| topic | NBS-LRR sequences RGAs F30 Plant genetics and breeding Q01 Food science and technology U30 Research methods Apricots DNA sequence |
| url | http://hdl.handle.net/20.500.11939/8468 https://www.actahort.org/books/717/717_22.htm |
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