Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops
Background: Bud dormancy is a phenological adaptation of temperate perennials that ensures survival under winter temperature conditions by ceasing growth and increasing cold hardiness. SHORT VEGETATIVE PHASE (SVP)-like factors, and particularly a subset of them named DORMANCY-ASSOCIATED MADS-BOX (DA...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Springer Nature
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8360 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-022-03856-7 |
| _version_ | 1855032776404762624 |
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| author | Quesada-Traver, Carles Lloret, Alba Carretero-Paulet, Lorenzo Badenes, María L. Ríos, Gabino |
| author_browse | Badenes, María L. Carretero-Paulet, Lorenzo Lloret, Alba Quesada-Traver, Carles Ríos, Gabino |
| author_facet | Quesada-Traver, Carles Lloret, Alba Carretero-Paulet, Lorenzo Badenes, María L. Ríos, Gabino |
| author_sort | Quesada-Traver, Carles |
| collection | ReDivia |
| description | Background: Bud dormancy is a phenological adaptation of temperate perennials that ensures survival under winter temperature conditions by ceasing growth and increasing cold hardiness. SHORT VEGETATIVE PHASE (SVP)-like factors, and particularly a subset of them named DORMANCY-ASSOCIATED MADS-BOX (DAM), are master regulators of bud dormancy in perennials, prominently Rosaceae crops widely adapted to varying environmental conditions. Results: SVP-like proteins from recently sequenced Rosaceae genomes were identifed and characterized using sequence, phylogenetic and synteny analysis tools. SVP-like proteins clustered in three clades (SVP1–3), with known DAM proteins located within SVP2 clade, which also included Arabidopsis AGAMOUS-LIKE 24 (AthAGL24). A more detailed study on these protein sequences led to the identifcation of a 15-amino acid long motif specifc to DAM proteins, which afected protein heteromerization properties by yeast two-hybrid system in peach PpeDAM6, and the unexpected fnding of predicted DAM-like genes in loquat, an evergreen species lacking winter dormancy. DAM gene expression in loquat trees was studied by quantitative PCR, associating with inforescence development and growth in varieties with contrasting fowering behaviour. Conclusions: Phylogenetic, synteny analyses and heterologous overexpression in the model plant Arabidopsis thaliana supported three major conclusions: 1) DAM proteins might have emerged from the SVP2 clade in the Amyg‑daloideae subfamily of Rosaceae; 2) a short DAM-specifc motif afects protein heteromerization, with a likely efect on DAM transcriptional targets and other functional features, providing a sequence signature for the DAM group of dormancy factors; 3) in agreement with other recent studies, DAM associates with inforescence development and growth, independently of the dormancy habit. |
| format | article |
| id | ReDivia8360 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia83602025-04-25T14:48:57Z Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops Quesada-Traver, Carles Lloret, Alba Carretero-Paulet, Lorenzo Badenes, María L. Ríos, Gabino SVP (gene family) evolution Plant phenology Bud development Winter dormancy F30 Plant genetics and breeding U30 Research methods F60 Plant physiology and biochemistry F40 Plant ecology Loquats Background: Bud dormancy is a phenological adaptation of temperate perennials that ensures survival under winter temperature conditions by ceasing growth and increasing cold hardiness. SHORT VEGETATIVE PHASE (SVP)-like factors, and particularly a subset of them named DORMANCY-ASSOCIATED MADS-BOX (DAM), are master regulators of bud dormancy in perennials, prominently Rosaceae crops widely adapted to varying environmental conditions. Results: SVP-like proteins from recently sequenced Rosaceae genomes were identifed and characterized using sequence, phylogenetic and synteny analysis tools. SVP-like proteins clustered in three clades (SVP1–3), with known DAM proteins located within SVP2 clade, which also included Arabidopsis AGAMOUS-LIKE 24 (AthAGL24). A more detailed study on these protein sequences led to the identifcation of a 15-amino acid long motif specifc to DAM proteins, which afected protein heteromerization properties by yeast two-hybrid system in peach PpeDAM6, and the unexpected fnding of predicted DAM-like genes in loquat, an evergreen species lacking winter dormancy. DAM gene expression in loquat trees was studied by quantitative PCR, associating with inforescence development and growth in varieties with contrasting fowering behaviour. Conclusions: Phylogenetic, synteny analyses and heterologous overexpression in the model plant Arabidopsis thaliana supported three major conclusions: 1) DAM proteins might have emerged from the SVP2 clade in the Amyg‑daloideae subfamily of Rosaceae; 2) a short DAM-specifc motif afects protein heteromerization, with a likely efect on DAM transcriptional targets and other functional features, providing a sequence signature for the DAM group of dormancy factors; 3) in agreement with other recent studies, DAM associates with inforescence development and growth, independently of the dormancy habit. 2022-10-06T08:10:43Z 2022-10-06T08:10:43Z 2022 article publishedVersion Quesada-Traver, C., Lloret, A., Carretero-Paulet, L., Badenes, M. L. & Ríos, G. (2022). Evolutionary origin and functional specialization of Dormancy-Associated MADS box (DAM) proteins in perennial crops. BMC Plant Biology, 22, 473. 1471-2229 http://hdl.handle.net/20.500.11939/8360 10.1186/s12870-022-03856-7 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-022-03856-7 en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PCI2020–120686-2//PREDICTING AND TUNING SEASONAL RESPONSES OF APPLE AND PEACH TO IMPROVE ORCHARD YIELD AND CLIMATE CHANGE RESILIENCE info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-114380RB-I00//ANALISIS GENETICO Y MOLECULAR DE LA LATENCIA DE LA YEMA EN MELOCOTONERO Y CIRUELO EUROPEO info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2020-113277GB-I00//NUEVAS APROXIMACIONES PARA EL ESTUDIO DE LA EVOLUCION DE GENES NUEVOS Y FUNCIONES GENICAS NOVEDOSAS EN PLANTAS This research was funded by MCIN/AEI, the European Union “NextGenerationEU”/PRTR and IVIA-FEDER with project references PCI2020–120686-2, PID2020-114380RB-I00, 52201 and 51308 to GR, a “Proyectos I+D Generación de Conocimiento” grant from the Spanish Ministry of Science and Innovation (grant code: PID2020-113277GB-I00) to LCP and by funds received by the “Sistema de Información Científca de Andalucía” Research Group id BIO359. AL was funded by a fellowship from Ministerio de Ciencia (Spanish Government). CQ-T was funded by a fellowship co-financed by the European Social Fund and the IVIA. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer Nature electronico |
| spellingShingle | SVP (gene family) evolution Plant phenology Bud development Winter dormancy F30 Plant genetics and breeding U30 Research methods F60 Plant physiology and biochemistry F40 Plant ecology Loquats Quesada-Traver, Carles Lloret, Alba Carretero-Paulet, Lorenzo Badenes, María L. Ríos, Gabino Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title | Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title_full | Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title_fullStr | Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title_full_unstemmed | Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title_short | Evolutionary origin and functional specialization of Dormancy‑Associated MADS box (DAM) proteins in perennial crops |
| title_sort | evolutionary origin and functional specialization of dormancy associated mads box dam proteins in perennial crops |
| topic | SVP (gene family) evolution Plant phenology Bud development Winter dormancy F30 Plant genetics and breeding U30 Research methods F60 Plant physiology and biochemistry F40 Plant ecology Loquats |
| url | http://hdl.handle.net/20.500.11939/8360 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-022-03856-7 |
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