Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants
Broad bean wilt virus 1 (BBWV-1) from the genus Fabavirus (family Secoviridae) infects many important crops worldwide. We have studied the subcellular alterations in leaf tissues of Nicotiana benthamiana and broad bean (Vicia faba) plants infected by BBWV-1 using transmission electron microscopy (TE...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8352 https://link.springer.com/article/10.1007/s10658-022-02564-0 |
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| author | Medina, Vicente Carpino, Caterina Elvira-González, Laura Rubio, Luis Galipienso, Luis |
| author_browse | Carpino, Caterina Elvira-González, Laura Galipienso, Luis Medina, Vicente Rubio, Luis |
| author_facet | Medina, Vicente Carpino, Caterina Elvira-González, Laura Rubio, Luis Galipienso, Luis |
| author_sort | Medina, Vicente |
| collection | ReDivia |
| description | Broad bean wilt virus 1 (BBWV-1) from the genus Fabavirus (family Secoviridae) infects many important crops worldwide. We have studied the subcellular alterations in leaf tissues of Nicotiana benthamiana and broad bean (Vicia faba) plants infected by BBWV-1 using transmission electron microscopy (TEM). In addition, we have studied the effect at the subcellular level of the BBWV-1 VP37 protein, which is a pathogenicity and host specificity determinant. For this, we agroinfiltrated N. benthamiana, and broad bean plants with two BBWV-1 infective clones, the wild type pBBWV1-wt and pBBWV1-G492C defective for VP37. Mesophyll cells infected with BBWV1-wt showed many subcellular alterations such as starch accumulation and plastoglobuli in chloroplasts, cytoplasmic vesiculation, plasmalemmasomes, vacuolar inclusion bodies, and presence of virus-like particles (VLPs) scattered in the cytoplasm in both N. benthamiana and broad bean. As differential effects, aggregated VLPs in vacuolar inclusion bodies and mitochondrial proliferation were observed in N. benthamiana but not in broad bean. TEM observation of pBBWV1-G492C infected tissues suggested that VP37 could be involved in the starch accumulation in both N. benthamiana and broad bean, and with the presence of aggregated VLPs in vacuolar inclusion bodies, plasmalemmasomes, and mitochondrial proliferation in N. benthamiana. Thus, subcellular alterations induced by BBWV-1 depended on the host species and the effect of VP37 expression was higher in N. benthamiana than in broad bean. |
| format | Artículo |
| id | ReDivia8352 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia83522025-04-25T14:48:57Z Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants Medina, Vicente Carpino, Caterina Elvira-González, Laura Rubio, Luis Galipienso, Luis Transmission electron microscopy (TEM) Broad bean wilt virus 1 (BBWV-1) Plastoglobuli Plasmalemmasomes Virus-like particles Nicotiana benthamiana H20 Plant diseases U30 Research methods Vicia faba Broad bean wilt virus 1 (BBWV-1) from the genus Fabavirus (family Secoviridae) infects many important crops worldwide. We have studied the subcellular alterations in leaf tissues of Nicotiana benthamiana and broad bean (Vicia faba) plants infected by BBWV-1 using transmission electron microscopy (TEM). In addition, we have studied the effect at the subcellular level of the BBWV-1 VP37 protein, which is a pathogenicity and host specificity determinant. For this, we agroinfiltrated N. benthamiana, and broad bean plants with two BBWV-1 infective clones, the wild type pBBWV1-wt and pBBWV1-G492C defective for VP37. Mesophyll cells infected with BBWV1-wt showed many subcellular alterations such as starch accumulation and plastoglobuli in chloroplasts, cytoplasmic vesiculation, plasmalemmasomes, vacuolar inclusion bodies, and presence of virus-like particles (VLPs) scattered in the cytoplasm in both N. benthamiana and broad bean. As differential effects, aggregated VLPs in vacuolar inclusion bodies and mitochondrial proliferation were observed in N. benthamiana but not in broad bean. TEM observation of pBBWV1-G492C infected tissues suggested that VP37 could be involved in the starch accumulation in both N. benthamiana and broad bean, and with the presence of aggregated VLPs in vacuolar inclusion bodies, plasmalemmasomes, and mitochondrial proliferation in N. benthamiana. Thus, subcellular alterations induced by BBWV-1 depended on the host species and the effect of VP37 expression was higher in N. benthamiana than in broad bean. 2022-09-21T12:28:18Z 2022-09-21T12:28:18Z 2022 article acceptedVersion Medina, V., Carpino, C., Elvira-González, L., Rubio, L. & Galipienso, L. (2022). Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants. European Journal of Plant Pathology, 164, 451-459. 1573-8469 (e-ISSN) 0929-1873 (print-ISSN) http://hdl.handle.net/20.500.11939/8352 10.1007/s10658-022-02564-0 https://link.springer.com/article/10.1007/s10658-022-02564-0 en This research work was supported by funds of the Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain (PROY-IVIA-2013/14 n°5426). C. Carpino was a recipient of predoctoral fellowships from the Italian government. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer electronico |
| spellingShingle | Transmission electron microscopy (TEM) Broad bean wilt virus 1 (BBWV-1) Plastoglobuli Plasmalemmasomes Virus-like particles Nicotiana benthamiana H20 Plant diseases U30 Research methods Vicia faba Medina, Vicente Carpino, Caterina Elvira-González, Laura Rubio, Luis Galipienso, Luis Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title | Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title_full | Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title_fullStr | Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title_full_unstemmed | Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title_short | Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants |
| title_sort | subcellular effects of broad bean wilt virus 1 infection in nicotiana benthamiana and broad bean plants |
| topic | Transmission electron microscopy (TEM) Broad bean wilt virus 1 (BBWV-1) Plastoglobuli Plasmalemmasomes Virus-like particles Nicotiana benthamiana H20 Plant diseases U30 Research methods Vicia faba |
| url | http://hdl.handle.net/20.500.11939/8352 https://link.springer.com/article/10.1007/s10658-022-02564-0 |
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