The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus

The zoophytophagous mirid Pilophorus clavatus has been recently identified inhabiting citrus orchards in the Mediterranean region, where it feeds on several important citrus pests. In this work, we investigated whether the plant feeding by P. clavatus could induce defensive responses in citrus plant...

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Autores principales: Dahmane, Meriem, Urbaneja, Alberto, Ruiz-Rivero, Omar, Alonso-Valiente, Miquel, Pérez-Hedo, Mertixell
Formato: article
Lenguaje:Inglés
Publicado: Springer 2022
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Acceso en línea:http://hdl.handle.net/20.500.11939/8343
https://link.springer.com/article/10.1007/s10340-022-01558-5
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author Dahmane, Meriem
Urbaneja, Alberto
Ruiz-Rivero, Omar
Alonso-Valiente, Miquel
Pérez-Hedo, Mertixell
author_browse Alonso-Valiente, Miquel
Dahmane, Meriem
Pérez-Hedo, Mertixell
Ruiz-Rivero, Omar
Urbaneja, Alberto
author_facet Dahmane, Meriem
Urbaneja, Alberto
Ruiz-Rivero, Omar
Alonso-Valiente, Miquel
Pérez-Hedo, Mertixell
author_sort Dahmane, Meriem
collection ReDivia
description The zoophytophagous mirid Pilophorus clavatus has been recently identified inhabiting citrus orchards in the Mediterranean region, where it feeds on several important citrus pests. In this work, we investigated whether the plant feeding by P. clavatus could induce defensive responses in citrus plants. Here, we show for the first time that the P. clavatus herbivory triggers the accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA) in citrus plants. Moreover, the SA and JA pathways enhanced plant defence mechanisms as the expression of genes encoding enzymes from both biosynthetic and responsive pathways were upregulated in P. clavatus-punctured plants. We also investigated whether the induced defences could affect the plant host selection of Tetranychus urticae and the predatory mites Phytoseilus persimilis and Neouseiulus californicus. Neither T. urticae nor N. californicus preferred the odour source emitted by intact or P. clavatus-punctured plants in a Y-tube olfactometer assay. However, P. persimilis were significantly attracted to P. clavatus-induced plants. The performance of T. urticae was also compared when mites were released on control or previously P. clavatus-induced plants. Compared to the control, the infestation of T. urticae was significantly reduced up to 70% on those citrus plants previously activated by P. clavatus. Our results show for the first time that feeding of P. clavatus on citrus plants can have a dual beneficial effect due to its known predatory action and, at the same time, by inducing the plant’s immune system.
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spelling ReDivia83432025-04-25T14:48:55Z The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus Dahmane, Meriem Urbaneja, Alberto Ruiz-Rivero, Omar Alonso-Valiente, Miquel Pérez-Hedo, Mertixell Plant host selection Herbivore-induced plant volatiles (HIPVs) Pilophorus clavatus H10 Pests of plants H20 Plant diseases U40 Surveying methods F60 Plant physiology and biochemistry H50 Miscellaneous plant disorders Biological control Tetranychus urticae Phytoseiulus persimilis Neoseiulus californicus citrus Plant hormones Salicylic acids Jasmonic acid Plant defense reactions The zoophytophagous mirid Pilophorus clavatus has been recently identified inhabiting citrus orchards in the Mediterranean region, where it feeds on several important citrus pests. In this work, we investigated whether the plant feeding by P. clavatus could induce defensive responses in citrus plants. Here, we show for the first time that the P. clavatus herbivory triggers the accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA) in citrus plants. Moreover, the SA and JA pathways enhanced plant defence mechanisms as the expression of genes encoding enzymes from both biosynthetic and responsive pathways were upregulated in P. clavatus-punctured plants. We also investigated whether the induced defences could affect the plant host selection of Tetranychus urticae and the predatory mites Phytoseilus persimilis and Neouseiulus californicus. Neither T. urticae nor N. californicus preferred the odour source emitted by intact or P. clavatus-punctured plants in a Y-tube olfactometer assay. However, P. persimilis were significantly attracted to P. clavatus-induced plants. The performance of T. urticae was also compared when mites were released on control or previously P. clavatus-induced plants. Compared to the control, the infestation of T. urticae was significantly reduced up to 70% on those citrus plants previously activated by P. clavatus. Our results show for the first time that feeding of P. clavatus on citrus plants can have a dual beneficial effect due to its known predatory action and, at the same time, by inducing the plant’s immune system. 2022-09-21T11:18:33Z 2022-09-21T11:18:33Z 2022 article acceptedVersion Dahmane, M., Urbaneja, A., Ruíz-Rivero, O., Alonso-Valiente, M. & Pérez-Hedo, M. (2022). The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus. Journal of Pest Science [published on-line, 14 September 2022]. 1612-4758 (print-ISSN) 1612-4766 (e-ISSN) http://hdl.handle.net/20.500.11939/8343 10.1007/s10340-022-01558-5 https://link.springer.com/article/10.1007/s10340-022-01558-5 en info:eu-repo/grantAgreement/MINECO//PID2020-113234RR-I00 The research leading to these results was partially funded by the Spanish Ministry of Economy and Competitiveness MINECO (PID2020-113234RR-I00) and the Conselleria d'Agricultura, Desenvolupament Rural, Emergència Climàtica i Transició Ecològica de la Generalitat Valenciana. M. D. was the recipient of a research fellowship funded by the Ministry of Higher Education and Scientific Research of Algeria (PNE). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer electronico
spellingShingle Plant host selection
Herbivore-induced plant volatiles (HIPVs)
Pilophorus clavatus
H10 Pests of plants
H20 Plant diseases
U40 Surveying methods
F60 Plant physiology and biochemistry
H50 Miscellaneous plant disorders
Biological control
Tetranychus urticae
Phytoseiulus persimilis
Neoseiulus californicus
citrus
Plant hormones
Salicylic acids
Jasmonic acid
Plant defense reactions
Dahmane, Meriem
Urbaneja, Alberto
Ruiz-Rivero, Omar
Alonso-Valiente, Miquel
Pérez-Hedo, Mertixell
The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title_full The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title_fullStr The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title_full_unstemmed The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title_short The zoophytophagous predator Pilophorus clavatus (Hemiptera: Miridae) induces plant defences in citrus
title_sort zoophytophagous predator pilophorus clavatus hemiptera miridae induces plant defences in citrus
topic Plant host selection
Herbivore-induced plant volatiles (HIPVs)
Pilophorus clavatus
H10 Pests of plants
H20 Plant diseases
U40 Surveying methods
F60 Plant physiology and biochemistry
H50 Miscellaneous plant disorders
Biological control
Tetranychus urticae
Phytoseiulus persimilis
Neoseiulus californicus
citrus
Plant hormones
Salicylic acids
Jasmonic acid
Plant defense reactions
url http://hdl.handle.net/20.500.11939/8343
https://link.springer.com/article/10.1007/s10340-022-01558-5
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