Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing
C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C. esculentus plant identified only one virus,...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8339 https://www.mdpi.com/2223-7747/11/15/2002 |
| _version_ | 1855032772751523840 |
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| author | Rubio, Luis Guinot-Moreno, Francisco J. Sanz-López, Carmen Galipienso, Luis |
| author_browse | Galipienso, Luis Guinot-Moreno, Francisco J. Rubio, Luis Sanz-López, Carmen |
| author_facet | Rubio, Luis Guinot-Moreno, Francisco J. Sanz-López, Carmen Galipienso, Luis |
| author_sort | Rubio, Luis |
| collection | ReDivia |
| description | C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C. esculentus plant identified only one virus, which could be the causal agent of this disease. The amino-acid comparison with viral sequences from GenBank and phylogenetic analyses indicated that this was a new species of genus Sobemovirus, and the name Xufa yellow dwarf virus was proposed. Completion with Sanger sequencing yielded a contig of 3072 nt corresponding to about 75% of the typical genome of sobemoviruses, including ORFs 2a (polyprotein-containing protease, VPG, and other proteins), 2b (RNA-dependent RNA polymerase), and 3 (coat protein). The nucleotide sequence was used to develop fast and accurate methods for the detection and quantification of xufa yellow dwarf virus (XYDV) based on reverse transcription (RT) and DNA amplification. XYDV was detected in leaves and tubers and showed a high incidence in the field in both symptomatic (almost 100%) and asymptomatic (70%) plants, but its accumulation was much higher in symptomatic plants. The relevance of these results for disease control was discussed. |
| format | article |
| id | ReDivia8339 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia83392025-04-25T14:48:55Z Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing Rubio, Luis Guinot-Moreno, Francisco J. Sanz-López, Carmen Galipienso, Luis Chufa RT-qPCR RT-LAMP Leaf yellowing Xufa yellow dwarf virus H20 Plant diseases U30 Research methods Tiger nut Cyperus esculentus Sobemoviruses leaf Symptoms Dwarfism C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C. esculentus plant identified only one virus, which could be the causal agent of this disease. The amino-acid comparison with viral sequences from GenBank and phylogenetic analyses indicated that this was a new species of genus Sobemovirus, and the name Xufa yellow dwarf virus was proposed. Completion with Sanger sequencing yielded a contig of 3072 nt corresponding to about 75% of the typical genome of sobemoviruses, including ORFs 2a (polyprotein-containing protease, VPG, and other proteins), 2b (RNA-dependent RNA polymerase), and 3 (coat protein). The nucleotide sequence was used to develop fast and accurate methods for the detection and quantification of xufa yellow dwarf virus (XYDV) based on reverse transcription (RT) and DNA amplification. XYDV was detected in leaves and tubers and showed a high incidence in the field in both symptomatic (almost 100%) and asymptomatic (70%) plants, but its accumulation was much higher in symptomatic plants. The relevance of these results for disease control was discussed. 2022-09-14T07:30:05Z 2022-09-14T07:30:05Z 2022 article publishedVersion Rubio, L., Guinot-Moreno, F. J., Sanz-López, C. & Galipienso, L. (2022). Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing. Plants, 11(15), 2002. 2223-7747 http://hdl.handle.net/20.500.11939/8339 10.3390/plants11152002 https://www.mdpi.com/2223-7747/11/15/2002 en info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51912 info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51903 info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE This research study was funded by Instituto Valenciano de Investigaciones Agrarias (IVIA) grants 51903, 51912, and 52202C. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Chufa RT-qPCR RT-LAMP Leaf yellowing Xufa yellow dwarf virus H20 Plant diseases U30 Research methods Tiger nut Cyperus esculentus Sobemoviruses leaf Symptoms Dwarfism Rubio, Luis Guinot-Moreno, Francisco J. Sanz-López, Carmen Galipienso, Luis Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title | Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title_full | Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title_fullStr | Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title_full_unstemmed | Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title_short | Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing |
| title_sort | discovery and diagnosis of a new sobemovirus infecting cyperus esculentus showing leaf yellow mosaic and dwarfism using small rna high throughput sequencing |
| topic | Chufa RT-qPCR RT-LAMP Leaf yellowing Xufa yellow dwarf virus H20 Plant diseases U30 Research methods Tiger nut Cyperus esculentus Sobemoviruses leaf Symptoms Dwarfism |
| url | http://hdl.handle.net/20.500.11939/8339 https://www.mdpi.com/2223-7747/11/15/2002 |
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