High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family
Cucumber mosaic virus (CMV; Cucumovirus, Bromoviridae) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variant...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8303 https://www.mdpi.com/2223-7747/11/13/1665 |
| _version_ | 1855032766403444736 |
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| author | Mrkvová, Michaela Hancinsky, Richard Predajna, Lukas Alaxin, Peter Achs, Adam Tomasechová, Jana Soltys, Katarína Mihálik, Daniel Olmos, Antonio Ruiz-García, Ana B. Glasa, Miroslav |
| author_browse | Achs, Adam Alaxin, Peter Glasa, Miroslav Hancinsky, Richard Mihálik, Daniel Mrkvová, Michaela Olmos, Antonio Predajna, Lukas Ruiz-García, Ana B. Soltys, Katarína Tomasechová, Jana |
| author_facet | Mrkvová, Michaela Hancinsky, Richard Predajna, Lukas Alaxin, Peter Achs, Adam Tomasechová, Jana Soltys, Katarína Mihálik, Daniel Olmos, Antonio Ruiz-García, Ana B. Glasa, Miroslav |
| author_sort | Mrkvová, Michaela |
| collection | ReDivia |
| description | Cucumber mosaic virus (CMV; Cucumovirus, Bromoviridae) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variants originated from the Papaveraceae species (oilseed poppy, common poppy and great celandine), previously poorly described as CMV natural hosts. Based on a BLAST search and phylogenetic analysis, the Slovak CMV isolates can be divided into two genetically different Groups, Ia and II, respectively. The SL50V variant, characterized by a divergent RNA2 sequence, potentially represents a reassortant variant. In four samples (T101, SL50V, CP2, MVU2-21), the presence of satellite CMV RNA was identified along with CMV. Although mechanically transmitted to experimental cucumber plants, the role of satellite RNA in the symptomatology observed could not be established due to a complex infection of original hosts with different viruses. |
| format | article |
| id | ReDivia8303 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia83032025-04-25T14:48:53Z High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family Mrkvová, Michaela Hancinsky, Richard Predajna, Lukas Alaxin, Peter Achs, Adam Tomasechová, Jana Soltys, Katarína Mihálik, Daniel Olmos, Antonio Ruiz-García, Ana B. Glasa, Miroslav High-throughput sequencing (HTS) Satellite RNA H20 Plant diseases F30 Plant genetics and breeding U30 Research methods Cmv (cucumber mosaic virus) Cucumoviruses Genetic diversity as resource Genomes Cucumber mosaic virus (CMV; Cucumovirus, Bromoviridae) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variants originated from the Papaveraceae species (oilseed poppy, common poppy and great celandine), previously poorly described as CMV natural hosts. Based on a BLAST search and phylogenetic analysis, the Slovak CMV isolates can be divided into two genetically different Groups, Ia and II, respectively. The SL50V variant, characterized by a divergent RNA2 sequence, potentially represents a reassortant variant. In four samples (T101, SL50V, CP2, MVU2-21), the presence of satellite CMV RNA was identified along with CMV. Although mechanically transmitted to experimental cucumber plants, the role of satellite RNA in the symptomatology observed could not be established due to a complex infection of original hosts with different viruses. 2022-08-22T06:33:09Z 2022-08-22T06:33:09Z 2022 article publishedVersion Mrkvová, M., Hančinský, R., Predajňa, L., Alaxin, P., Achs, A., Tomašechová, J. et al. (2022). High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family. Plants, 11(13), 1665. 2223-7747 http://hdl.handle.net/20.500.11939/8303 10.3390/plants11131665 https://www.mdpi.com/2223-7747/11/13/1665 en This research was supported by grants APVV-18-0005 and APVV-20-0015 from the Slovak Research and Development Agency and VEGA 2/0030/20 from the Scientific Grant Agency of the Ministry of Education and Slovak Academy of Sciences. M.M., P.A., D.M. and M.G. acknowledge support from the Operational Programme Integrated Infrastructure “Addressing the societal threats posed by the COVID-19 pandemic” (313011ASN4) co-financed by the European Regional Development Fund. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | High-throughput sequencing (HTS) Satellite RNA H20 Plant diseases F30 Plant genetics and breeding U30 Research methods Cmv (cucumber mosaic virus) Cucumoviruses Genetic diversity as resource Genomes Mrkvová, Michaela Hancinsky, Richard Predajna, Lukas Alaxin, Peter Achs, Adam Tomasechová, Jana Soltys, Katarína Mihálik, Daniel Olmos, Antonio Ruiz-García, Ana B. Glasa, Miroslav High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title | High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title_full | High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title_fullStr | High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title_full_unstemmed | High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title_short | High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family |
| title_sort | high throughput sequencing discloses the cucumber mosaic virus cmv diversity in slovakia and reveals new hosts of cmv from the papaveraceae family |
| topic | High-throughput sequencing (HTS) Satellite RNA H20 Plant diseases F30 Plant genetics and breeding U30 Research methods Cmv (cucumber mosaic virus) Cucumoviruses Genetic diversity as resource Genomes |
| url | http://hdl.handle.net/20.500.11939/8303 https://www.mdpi.com/2223-7747/11/13/1665 |
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