Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does
Superovulation treatments aim to stimulate multifollicular recruitment, maximizing the number of oocytes or transferable embryos produced. Factors associated with the superovulation protocol, female characteristics and many other factors are determinants in the number and quality of oocytes obtained...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8301 https://www.sciencedirect.com/science/article/abs/pii/S0093691X2200214X?via%3Dihub#! |
| _version_ | 1855032766053220352 |
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| author | Vicente, José Salvador Marco-Jiménez, Francisco Pérez-García, M. Naturil-Alfonso, C. Penaranda, David S. Viudes-De-Castro, María P. |
| author_browse | Marco-Jiménez, Francisco Naturil-Alfonso, C. Penaranda, David S. Pérez-García, M. Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Vicente, José Salvador Marco-Jiménez, Francisco Pérez-García, M. Naturil-Alfonso, C. Penaranda, David S. Viudes-De-Castro, María P. |
| author_sort | Vicente, José Salvador |
| collection | ReDivia |
| description | Superovulation treatments aim to stimulate multifollicular recruitment, maximizing the number of oocytes or transferable embryos produced. Factors associated with the superovulation protocol, female characteristics and many other factors are determinants in the number and quality of oocytes obtained. An accurate way to assess oocyte quality more precise than morphological appearance is genetic expression. The present study aims to compare the response of nulliparous and multiparous females to superovulatory stimulation, studying its effect on the expression of some genes associated with the activation, growth, development and oocyte-embryo transition of oocytes, as well as its impact on in vivo embryonic development and viability rate at birth. In a first experiment, the effect of stimulation treatment on the ovulation response and the expression of the MSY2, MATER, ITPR1, ITPR2, ITPR3, eIF4E, PAR1, PAPOL-A, PAPOL-G, ZAR1 and YY1 genes in nulliparous and multiparous females were determined. In a second experiment, the implantation and viability at birth of embryos from superovulated nulliparous and multiparous females were analysed. The ovulation rate was significantly higher in the superovulation groups than in the control groups. The ovulation rate was significantly increased in nulliparous females compared with multiparous does. From the eleven genes analysed, only the expression of MATER, PAPOL-A, PAPOL-G and ZAR-1 genes was shown to be different among experimental groups. Finally, in terms of implantation rate and viability at birth, the nulliparous control group showed better results than the rest of the groups. Both hyperstimulation treatment and reproductive female's history seem to alter the transcriptome of important genes related to oocyte maturation and competence acquisition, affecting in vivo embryo viability |
| format | article |
| id | ReDivia8301 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia83012025-04-25T14:48:53Z Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does Vicente, José Salvador Marco-Jiménez, Francisco Pérez-García, M. Naturil-Alfonso, C. Penaranda, David S. Viudes-De-Castro, María P. Oocytes quality In vivo Nulliparous females Multiparous females L10 Animal genetics and breeding L53 Animal physiology - Reproduction U40 Surveying methods Superovulation Oocytes Embryo transfer Gene expression Rabbits Superovulation treatments aim to stimulate multifollicular recruitment, maximizing the number of oocytes or transferable embryos produced. Factors associated with the superovulation protocol, female characteristics and many other factors are determinants in the number and quality of oocytes obtained. An accurate way to assess oocyte quality more precise than morphological appearance is genetic expression. The present study aims to compare the response of nulliparous and multiparous females to superovulatory stimulation, studying its effect on the expression of some genes associated with the activation, growth, development and oocyte-embryo transition of oocytes, as well as its impact on in vivo embryonic development and viability rate at birth. In a first experiment, the effect of stimulation treatment on the ovulation response and the expression of the MSY2, MATER, ITPR1, ITPR2, ITPR3, eIF4E, PAR1, PAPOL-A, PAPOL-G, ZAR1 and YY1 genes in nulliparous and multiparous females were determined. In a second experiment, the implantation and viability at birth of embryos from superovulated nulliparous and multiparous females were analysed. The ovulation rate was significantly higher in the superovulation groups than in the control groups. The ovulation rate was significantly increased in nulliparous females compared with multiparous does. From the eleven genes analysed, only the expression of MATER, PAPOL-A, PAPOL-G and ZAR-1 genes was shown to be different among experimental groups. Finally, in terms of implantation rate and viability at birth, the nulliparous control group showed better results than the rest of the groups. Both hyperstimulation treatment and reproductive female's history seem to alter the transcriptome of important genes related to oocyte maturation and competence acquisition, affecting in vivo embryo viability 2022-08-17T07:47:38Z 2022-08-17T07:47:38Z 2022 article acceptedVersion Vicente, J. S., Marco-Jiménez, F., Pérez-García, M., Naturil-Alfonso, C., Peñaranda, D. S. & Viudes-de-Castro, M. P. (2022). Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does. Theriogenology, 189, 53-58. 0093-691X http://hdl.handle.net/20.500.11939/8301 10.1016/j.theriogenology.2022.06.003 https://www.sciencedirect.com/science/article/abs/pii/S0093691X2200214X?via%3Dihub#! en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/AGL2017-85162-C2-1-R//MEJORA GENETICA DEL CONEJO DE CARNE: ESTRATEGIAS PARA INCREMENTAR LA EFICACIA DE LA MEJORA, REPRODUCCION Y SALUD DE LINEAS PATERNALES This research was supported by the Spanish Research project funded by the Ministry of Economy, Industry and Competitiveness (MINECO, Spain) AGL2017-85162-C2-1-R. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Elsevier electronico |
| spellingShingle | Oocytes quality In vivo Nulliparous females Multiparous females L10 Animal genetics and breeding L53 Animal physiology - Reproduction U40 Surveying methods Superovulation Oocytes Embryo transfer Gene expression Rabbits Vicente, José Salvador Marco-Jiménez, Francisco Pérez-García, M. Naturil-Alfonso, C. Penaranda, David S. Viudes-De-Castro, María P. Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title | Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title_full | Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title_fullStr | Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title_full_unstemmed | Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title_short | Oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| title_sort | oocyte quality and in vivo embryo survival after ovarian stimulation in nulliparous and multiparous rabbit does |
| topic | Oocytes quality In vivo Nulliparous females Multiparous females L10 Animal genetics and breeding L53 Animal physiology - Reproduction U40 Surveying methods Superovulation Oocytes Embryo transfer Gene expression Rabbits |
| url | http://hdl.handle.net/20.500.11939/8301 https://www.sciencedirect.com/science/article/abs/pii/S0093691X2200214X?via%3Dihub#! |
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