From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes
Citrus is the most cultivated fruit crop worldwide. The modern citrus industry needs new bioproducts to overcome phytopathological threats, tolerate stresses and increase yield and quality. Mutualistic microbes from roots significantly impact host physiology and health and are a potentially benefici...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8245 https://www.sciencedirect.com/science/article/pii/S1871678422000395?via%3Dihub |
| _version_ | 1855032756042465280 |
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| author | Penyalver, Ramón Roesch, Luis F. W. Piquer-Salcedo, Jaime E. Forner-Giner, María A. Alguacil, María M. |
| author_browse | Alguacil, María M. Forner-Giner, María A. Penyalver, Ramón Piquer-Salcedo, Jaime E. Roesch, Luis F. W. |
| author_facet | Penyalver, Ramón Roesch, Luis F. W. Piquer-Salcedo, Jaime E. Forner-Giner, María A. Alguacil, María M. |
| author_sort | Penyalver, Ramón |
| collection | ReDivia |
| description | Citrus is the most cultivated fruit crop worldwide. The modern citrus industry needs new bioproducts to overcome phytopathological threats, tolerate stresses and increase yield and quality. Mutualistic microbes from roots significantly impact host physiology and health and are a potentially beneficial resource. The bacterial microbiome can be surveyed to select potentially host-beneficial microbes. To achieve this goal, a prevalent “core-citrus” bacterial microbiome was obtained by picking those operational taxonomic units (OTUs) shared among samples within and across two Citrus rootstock genotypes grown in the same soil for more than 20 years. A sub-selection of main OTUs from the defined "core-citrus" microbiome was made based on abundance, host-enriched versus bulk soil, and rhizosphere-indicator species. In parallel, an extensive census of the cultivable microbiota was performed to collect a large number of bacterial citrus isolates. Metataxonomic data were linked to cultured microbes, matching 16S rRNA gene sequences from bacterial isolates with those counterpart OTU reference sequences from the selected bacterial "core-citrus" microbiome. This approach allowed selection of potentially host-beneficial bacteria to mine for agricultural probiotics in future biotechnological applications required for the citrus industry. |
| format | article |
| id | ReDivia8245 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia82452025-04-25T14:48:53Z From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes Penyalver, Ramón Roesch, Luis F. W. Piquer-Salcedo, Jaime E. Forner-Giner, María A. Alguacil, María M. Citrus microbiomes Metataxonomics Culturomics Rhizospheric bacteria Beneficial bacteria Agricultural probiotics H20 Plant diseases U40 Surveying methods F30 Plant genetics and breeding A50 Agricultural research P34 Soil biology Citrus is the most cultivated fruit crop worldwide. The modern citrus industry needs new bioproducts to overcome phytopathological threats, tolerate stresses and increase yield and quality. Mutualistic microbes from roots significantly impact host physiology and health and are a potentially beneficial resource. The bacterial microbiome can be surveyed to select potentially host-beneficial microbes. To achieve this goal, a prevalent “core-citrus” bacterial microbiome was obtained by picking those operational taxonomic units (OTUs) shared among samples within and across two Citrus rootstock genotypes grown in the same soil for more than 20 years. A sub-selection of main OTUs from the defined "core-citrus" microbiome was made based on abundance, host-enriched versus bulk soil, and rhizosphere-indicator species. In parallel, an extensive census of the cultivable microbiota was performed to collect a large number of bacterial citrus isolates. Metataxonomic data were linked to cultured microbes, matching 16S rRNA gene sequences from bacterial isolates with those counterpart OTU reference sequences from the selected bacterial "core-citrus" microbiome. This approach allowed selection of potentially host-beneficial bacteria to mine for agricultural probiotics in future biotechnological applications required for the citrus industry. 2022-06-23T07:17:20Z 2022-06-23T07:17:20Z 2022 article publishedVersion Penyalver, R., Roesch, L. F. W., Piquer-Salcedo, J. E., Forner-Giner, M. A. & Alguacil, M. M. (2022). From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes. New Biotechnology, 70, 116-128. 1871-6784 http://hdl.handle.net/20.500.11939/8245 10.1016/j.nbt.2022.06.002 https://www.sciencedirect.com/science/article/pii/S1871678422000395?via%3Dihub en info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2015–00087-C2-01 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-91803-EXP info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018–098379-R-I00//OBTENCION, SELECCION, CARACTERIZACION Y EVALUACION DE NUEVOS PATRONES DE CITRICOS This work was supported by grants RTA2015–00087-C2–01, AGL2017–91803-EXP and RTI2018–098379-R-I00 from Spanish Agency of Science (AEI, Spain) and co-funded by the European Social Fund to E. Marco-Noales, R. Penyalver and M.A. Forner-Giner, respectively. Part of this work was also conducted during a visiting stay of R. Penyalver at the Universidade Federal do Pampa (Unipampa), Sao Gabriel, RS. Brazil funded by the “Programa Movilidad” from AEI (Spain) PRX15/00253. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Elsevier electronico |
| spellingShingle | Citrus microbiomes Metataxonomics Culturomics Rhizospheric bacteria Beneficial bacteria Agricultural probiotics H20 Plant diseases U40 Surveying methods F30 Plant genetics and breeding A50 Agricultural research P34 Soil biology Penyalver, Ramón Roesch, Luis F. W. Piquer-Salcedo, Jaime E. Forner-Giner, María A. Alguacil, María M. From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title | From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title_full | From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title_fullStr | From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title_full_unstemmed | From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title_short | From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes |
| title_sort | from the bacterial citrus microbiome to the selection of potentially host beneficial microbes |
| topic | Citrus microbiomes Metataxonomics Culturomics Rhizospheric bacteria Beneficial bacteria Agricultural probiotics H20 Plant diseases U40 Surveying methods F30 Plant genetics and breeding A50 Agricultural research P34 Soil biology |
| url | http://hdl.handle.net/20.500.11939/8245 https://www.sciencedirect.com/science/article/pii/S1871678422000395?via%3Dihub |
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