A New Indexing Method for Cachexia
The utilization of nucleic acid extraction and polyacrylamide gel electrophoresis as an alternative to the Parson's Special bioassay for routine indexing of cachexia was evaluated. Three field sources of citrus viroids and three greenhouse controls were graft inoculated on Arizona Etrog citron. Inoc...
| Autores principales: | , , , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
IOCV
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8200 https://iocv.ucr.edu/sites/default/files/2020-06/11th224_229.pdf |
| _version_ | 1855492444244672512 |
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| author | Durán-Vila, Núria Pina, José A. Molins, M. Navarro, Luis |
| author2 | Brlansky, R. H. |
| author_browse | Brlansky, R. H. Durán-Vila, Núria Molins, M. Navarro, Luis Pina, José A. |
| author_facet | Brlansky, R. H. Durán-Vila, Núria Pina, José A. Molins, M. Navarro, Luis |
| author_sort | Durán-Vila, Núria |
| collection | ReDivia |
| description | The utilization of nucleic acid extraction and polyacrylamide gel electrophoresis as an alternative to the Parson's Special bioassay for routine indexing of cachexia was evaluated. Three field sources of citrus viroids and three greenhouse controls were graft inoculated on Arizona Etrog citron. Inoculated citrons were periodically analyzed by nucleic acid extraction and sequential gel electrophoresis. The citrus cachexia viroid (CCaV) was detected after 3 months in all the plants tested regardless of the source. Three electrophoresis systems were also compared. The proposed method combines the desirable characteristics of citron as an excellent host for viroid replication and the sensitivity of electrophoresis and silver staining for viroid detection. The method is now routinely used in the Citrus Sanitation Program in Spain. |
| format | Objeto de conferencia |
| id | ReDivia8200 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia82002025-04-25T14:54:03Z A New Indexing Method for Cachexia Durán-Vila, Núria Pina, José A. Molins, M. Navarro, Luis Brlansky, R. H. Citrus cachexia viroid Citrus viroids Xyloporosis H20 Plant diseases A50 Agricultural research U30 Research methods The utilization of nucleic acid extraction and polyacrylamide gel electrophoresis as an alternative to the Parson's Special bioassay for routine indexing of cachexia was evaluated. Three field sources of citrus viroids and three greenhouse controls were graft inoculated on Arizona Etrog citron. Inoculated citrons were periodically analyzed by nucleic acid extraction and sequential gel electrophoresis. The citrus cachexia viroid (CCaV) was detected after 3 months in all the plants tested regardless of the source. Three electrophoresis systems were also compared. The proposed method combines the desirable characteristics of citron as an excellent host for viroid replication and the sensitivity of electrophoresis and silver staining for viroid detection. The method is now routinely used in the Citrus Sanitation Program in Spain. 2022-06-07T10:56:52Z 2022-06-07T10:56:52Z 1991 conferenceObject Duran-Vila, N., Pina, J. A., Molins, M. I. & Navarro, L. (1991). A new indexing method for cachexia. Proceedings of the Eleventh Conference of the IOCV, 224-229. 2313-5123 http://hdl.handle.net/20.500.11939/8200 https://iocv.ucr.edu/sites/default/files/2020-06/11th224_229.pdf en 1989-11-06 Eleventh Conference of the International Organization of Citrus Virologists Orlando, Florida Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Citrus cachexia viroid Citrus viroids Xyloporosis H20 Plant diseases A50 Agricultural research U30 Research methods Durán-Vila, Núria Pina, José A. Molins, M. Navarro, Luis A New Indexing Method for Cachexia |
| title | A New Indexing Method for Cachexia |
| title_full | A New Indexing Method for Cachexia |
| title_fullStr | A New Indexing Method for Cachexia |
| title_full_unstemmed | A New Indexing Method for Cachexia |
| title_short | A New Indexing Method for Cachexia |
| title_sort | new indexing method for cachexia |
| topic | Citrus cachexia viroid Citrus viroids Xyloporosis H20 Plant diseases A50 Agricultural research U30 Research methods |
| url | http://hdl.handle.net/20.500.11939/8200 https://iocv.ucr.edu/sites/default/files/2020-06/11th224_229.pdf |
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