The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus
A consensus catalogue for the grouping of citrus viroids (CV) utilizing the physical parameters of size and nucleotide sequence homology as tested with group-specific cDNA probes has been furthered developed. Analysis of cachexia disease sources by sequential polyacrylamide gel electrophoresis (sPAG...
| Autores principales: | , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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IOCV
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8199 https://iocv.ucr.edu/sites/default/files/2020-06/11th178_188.pdf |
| _version_ | 1855492444067463168 |
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| author | Semancik, J. S. Durán-Vila, Núria |
| author2 | Brlansky, R. H. |
| author_browse | Brlansky, R. H. Durán-Vila, Núria Semancik, J. S. |
| author_facet | Brlansky, R. H. Semancik, J. S. Durán-Vila, Núria |
| author_sort | Semancik, J. S. |
| collection | ReDivia |
| description | A consensus catalogue for the grouping of citrus viroids (CV) utilizing the physical parameters of size and nucleotide sequence homology as tested with group-specific cDNA probes has been furthered developed. Analysis of cachexia disease sources by sequential polyacrylamide gel electrophoresis (sPAGE) has revealed a third member of the CV-I1 Group, designated as CV-IIc. A viroid characterized by a similar size and sequence homology is common to all "xyloporosis" disease sources analyzed. Chrysanthemum, which has been identified as a selective host for CEV and the Group I1 viroids, produces a leaf spotting reaction when inoculated with CV-IIa, the viroid inducing the most mild reaction in citron. Mixed infections of viroids from different groups has indicated a range of responses on citron including: 1) both enhanced and delayed severe dwarfing symptoms of CEV, 2) synergism resulting in severe dwarfing and epinasty mimicking CEV symptoms, and 3) a variation in leaf symptoms ranging from no effect to severe epinasty. Field testing of pure citrus viroids and mixed viroid inoculations of commercial scion on exocortis sensitive rootstock combinations has been initiated. Analysis of the viroid profiles of citrons inoculated with field sources of some diseases of suspected viroid etiology such as, "xyloporosis" of Palestine sweet lime, "gummy bark" of navel orange, and "Kassala" of grapefruit, indicated the presence of a complex pattern of several viroids. |
| format | Objeto de conferencia |
| id | ReDivia8199 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia81992025-04-25T14:54:04Z The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus Semancik, J. S. Durán-Vila, Núria Brlansky, R. H. Citrus viroids Etrog citron cDNA viroid probes Sequential PAGE Exocortis Cachexia H20 Plant diseases U30 Research methods Molecular hybridization Electrophoresis A consensus catalogue for the grouping of citrus viroids (CV) utilizing the physical parameters of size and nucleotide sequence homology as tested with group-specific cDNA probes has been furthered developed. Analysis of cachexia disease sources by sequential polyacrylamide gel electrophoresis (sPAGE) has revealed a third member of the CV-I1 Group, designated as CV-IIc. A viroid characterized by a similar size and sequence homology is common to all "xyloporosis" disease sources analyzed. Chrysanthemum, which has been identified as a selective host for CEV and the Group I1 viroids, produces a leaf spotting reaction when inoculated with CV-IIa, the viroid inducing the most mild reaction in citron. Mixed infections of viroids from different groups has indicated a range of responses on citron including: 1) both enhanced and delayed severe dwarfing symptoms of CEV, 2) synergism resulting in severe dwarfing and epinasty mimicking CEV symptoms, and 3) a variation in leaf symptoms ranging from no effect to severe epinasty. Field testing of pure citrus viroids and mixed viroid inoculations of commercial scion on exocortis sensitive rootstock combinations has been initiated. Analysis of the viroid profiles of citrons inoculated with field sources of some diseases of suspected viroid etiology such as, "xyloporosis" of Palestine sweet lime, "gummy bark" of navel orange, and "Kassala" of grapefruit, indicated the presence of a complex pattern of several viroids. 2022-06-07T10:54:43Z 2022-06-07T10:54:43Z 1991 conferenceObject Semancik, J. S. & Duran-Vila, N. (1991). The grouping of citrus viroids: additional physical and biological determinants and relationships with diseases of citrus. Proceedings of the Eleventh Conference of the IOCV, 178-188. 2313-5123 http://hdl.handle.net/20.500.11939/8199 https://iocv.ucr.edu/sites/default/files/2020-06/11th178_188.pdf en 1989-11-06 Eleventh Conference of the International Organization of Citrus Virologists Orlando, Florida These studies were supported in part by the U.S.-Spain Joint Committee for Scientific and Technological Cooperation and the U.S.-Israel Binational Agricultural Research and Development Fund. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Citrus viroids Etrog citron cDNA viroid probes Sequential PAGE Exocortis Cachexia H20 Plant diseases U30 Research methods Molecular hybridization Electrophoresis Semancik, J. S. Durán-Vila, Núria The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title | The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title_full | The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title_fullStr | The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title_full_unstemmed | The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title_short | The Grouping of Citrus Viroids: Additional Physical and Biological Determinants and Relationships with Diseases of Citrus |
| title_sort | grouping of citrus viroids additional physical and biological determinants and relationships with diseases of citrus |
| topic | Citrus viroids Etrog citron cDNA viroid probes Sequential PAGE Exocortis Cachexia H20 Plant diseases U30 Research methods Molecular hybridization Electrophoresis |
| url | http://hdl.handle.net/20.500.11939/8199 https://iocv.ucr.edu/sites/default/files/2020-06/11th178_188.pdf |
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