Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus
Domesticated citrus varieties are woody perennials and interspecific hybrid crops of global economic and nutritional importance. The citrus fruit “hesperidium” is a unique morphological innovation not found in any other plant lineage. Efforts to improve the nutritional quality of the fruit are predi...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8196 https://academic.oup.com/plphys/article-abstract/187/2/829/6308950 |
| _version_ | 1855492443503329280 |
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| author | Zhu, Kaijie Zheng, Xiongjie Ye, Junli Huang, Yue Chen, Hongyan Mei, Xuehan Xie, Zhongzhou Cao, Lixin Zeng, Yunliu Larkin, Robert M. Xu, Qiang Pérez-Román, Estela Talón, Manuel Zumajo-Cardona, Cecilia Wurtzel, Eleanore T. Deng, Xiuxin |
| author_browse | Cao, Lixin Chen, Hongyan Deng, Xiuxin Huang, Yue Larkin, Robert M. Mei, Xuehan Pérez-Román, Estela Talón, Manuel Wurtzel, Eleanore T. Xie, Zhongzhou Xu, Qiang Ye, Junli Zeng, Yunliu Zheng, Xiongjie Zhu, Kaijie Zumajo-Cardona, Cecilia |
| author_facet | Zhu, Kaijie Zheng, Xiongjie Ye, Junli Huang, Yue Chen, Hongyan Mei, Xuehan Xie, Zhongzhou Cao, Lixin Zeng, Yunliu Larkin, Robert M. Xu, Qiang Pérez-Román, Estela Talón, Manuel Zumajo-Cardona, Cecilia Wurtzel, Eleanore T. Deng, Xiuxin |
| author_sort | Zhu, Kaijie |
| collection | ReDivia |
| description | Domesticated citrus varieties are woody perennials and interspecific hybrid crops of global economic and nutritional importance. The citrus fruit “hesperidium” is a unique morphological innovation not found in any other plant lineage. Efforts to improve the nutritional quality of the fruit are predicated on understanding the underlying regulatory mechanisms responsible for fruit development, including temporal control of chlorophyll degradation and carotenoid biosynthesis. Here, we investigated the molecular basis of the navel orange (Citrus sinensis) brown flavedo mutation, which conditions flavedo that is brown instead of orange. To overcome the limitations of using traditional genetic approaches in citrus and other woody perennials, we developed a strategy to elucidate the underlying genetic lesion. We used a multi-omics approach to collect data from several genetic sources and plant chimeras to successfully decipher this mutation. The multi-omics strategy applied here will be valuable in driving future gene discovery efforts in citrus as well as in other woody perennial plants. The comparison of transcriptomic and genomic data from multiple genotypes and plant sectors revealed an underlying lesion in the gene encoding STAY-GREEN (SGR) protein, which simultaneously regulates carotenoid biosynthesis and chlorophyll degradation. However, unlike SGR of other plant species, we found that the carotenoid and chlorophyll regulatory activities could be uncoupled in the case of certain SGR alleles in citrus and thus we propose a model for the molecular mechanism underlying the brown flavedo phenotype. The economic and nutritional value of citrus makes these findings of wide interest. The strategy implemented, and the results obtained, constitute an advance for agro-industry by driving opportunities for citrus crop improvement. |
| format | Artículo |
| id | ReDivia8196 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia81962025-04-25T14:48:51Z Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus Zhu, Kaijie Zheng, Xiongjie Ye, Junli Huang, Yue Chen, Hongyan Mei, Xuehan Xie, Zhongzhou Cao, Lixin Zeng, Yunliu Larkin, Robert M. Xu, Qiang Pérez-Román, Estela Talón, Manuel Zumajo-Cardona, Cecilia Wurtzel, Eleanore T. Deng, Xiuxin Citrus varieties Fruit quality Multi-omics strategy STAY-GREEN protein Citrus crops improvement F30 Plant genetics and breeding E21 Agro-industry F60 Plant physiology and biochemistry Citrus Plant domestication Chlorophylls Carotenoids Citrus sinensis Domesticated citrus varieties are woody perennials and interspecific hybrid crops of global economic and nutritional importance. The citrus fruit “hesperidium” is a unique morphological innovation not found in any other plant lineage. Efforts to improve the nutritional quality of the fruit are predicated on understanding the underlying regulatory mechanisms responsible for fruit development, including temporal control of chlorophyll degradation and carotenoid biosynthesis. Here, we investigated the molecular basis of the navel orange (Citrus sinensis) brown flavedo mutation, which conditions flavedo that is brown instead of orange. To overcome the limitations of using traditional genetic approaches in citrus and other woody perennials, we developed a strategy to elucidate the underlying genetic lesion. We used a multi-omics approach to collect data from several genetic sources and plant chimeras to successfully decipher this mutation. The multi-omics strategy applied here will be valuable in driving future gene discovery efforts in citrus as well as in other woody perennial plants. The comparison of transcriptomic and genomic data from multiple genotypes and plant sectors revealed an underlying lesion in the gene encoding STAY-GREEN (SGR) protein, which simultaneously regulates carotenoid biosynthesis and chlorophyll degradation. However, unlike SGR of other plant species, we found that the carotenoid and chlorophyll regulatory activities could be uncoupled in the case of certain SGR alleles in citrus and thus we propose a model for the molecular mechanism underlying the brown flavedo phenotype. The economic and nutritional value of citrus makes these findings of wide interest. The strategy implemented, and the results obtained, constitute an advance for agro-industry by driving opportunities for citrus crop improvement. 2022-06-07T10:51:54Z 2022-06-07T10:51:54Z 2021 article publishedVersion Zhu, K., Zheng, X., Ye, J., Huang, Y., Chen, H., Mei, X. et al. (2021). Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus. Plant Physiology, 187(2), 829-845. 0032-0889 (print ISSN) 1532-2548 (eISSN) http://hdl.handle.net/20.500.11939/8196 10.1093/plphys/kiab291 https://academic.oup.com/plphys/article-abstract/187/2/829/6308950 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Oxford University Press electronico |
| spellingShingle | Citrus varieties Fruit quality Multi-omics strategy STAY-GREEN protein Citrus crops improvement F30 Plant genetics and breeding E21 Agro-industry F60 Plant physiology and biochemistry Citrus Plant domestication Chlorophylls Carotenoids Citrus sinensis Zhu, Kaijie Zheng, Xiongjie Ye, Junli Huang, Yue Chen, Hongyan Mei, Xuehan Xie, Zhongzhou Cao, Lixin Zeng, Yunliu Larkin, Robert M. Xu, Qiang Pérez-Román, Estela Talón, Manuel Zumajo-Cardona, Cecilia Wurtzel, Eleanore T. Deng, Xiuxin Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title | Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title_full | Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title_fullStr | Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title_full_unstemmed | Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title_short | Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus |
| title_sort | regulation of carotenoid and chlorophyll pools in hesperidia anatomically unique fruits found only in citrus |
| topic | Citrus varieties Fruit quality Multi-omics strategy STAY-GREEN protein Citrus crops improvement F30 Plant genetics and breeding E21 Agro-industry F60 Plant physiology and biochemistry Citrus Plant domestication Chlorophylls Carotenoids Citrus sinensis |
| url | http://hdl.handle.net/20.500.11939/8196 https://academic.oup.com/plphys/article-abstract/187/2/829/6308950 |
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