Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species
Seedlessness is one of the most important agronomic traits in mandarins on the fresh fruit market. Creation of triploid plants is an important breeding strategy for development of new commercial varieties of seedless citrus. To this end, one strategy is to perform sexual hybridizations, with tetrapl...
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| Formato: | article |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8188 https://www.frontiersin.org/articles/10.3389/fpls.2022.862813/full |
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| author | Lora, Jorge García-Lor, Andrés Aleza, Pablo |
| author_browse | Aleza, Pablo García-Lor, Andrés Lora, Jorge |
| author_facet | Lora, Jorge García-Lor, Andrés Aleza, Pablo |
| author_sort | Lora, Jorge |
| collection | ReDivia |
| description | Seedlessness is one of the most important agronomic traits in mandarins on the fresh fruit market. Creation of triploid plants is an important breeding strategy for development of new commercial varieties of seedless citrus. To this end, one strategy is to perform sexual hybridizations, with tetraploid genotypes as male parents. However, while seed development has been widely studied in citrus, knowledge of key steps such as microsporogenesis and microgametogenesis, is scarce, especially in polyploids. Therefore, we performed a study on the effect of ploidy level on pollen development by including diploid and tetraploid (double diploid) genotypes with different degrees of pollen performance. A comprehensive study on the pollen ontogeny of diploid and doubled diploid “Sanguinelli” blood orange and “Clemenules” clementine was performed, with focus on pollen grain germination in vitro and in planta, morphology of mature pollen grains by scanning electron microscopy (SEM), cytochemical characterization of carbohydrates by periodic acid–Shiff staining, and specific cell wall components revealed by immunolocalization. During microsporogenesis, the main difference between diploid and doubled diploid genotypes was cell area, which was larger in doubled diploid genotypes. However, after increase in size and vacuolization of microspores, but before mitosis I, doubled diploid “Clemenules” clementine showed drastic differences in shape, cell area, and starch hydrolysis, which resulted in shrinkage of pollen grains. The loss of fertility in doubled diploid “Clemenules” clementine is mainly due to lack of carbohydrate accumulation in pollen during microgametogenesis, especially starch content, which led to pollen grain abortion. All these changes make the pollen of this genotype unviable and very difficult to use as a male parent in sexual hybridization with the objective of recovering large progenies of triploid hybrids. |
| format | article |
| id | ReDivia8188 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia81882025-04-25T14:48:50Z Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species Lora, Jorge García-Lor, Andrés Aleza, Pablo Pollen performance Microsporogenesis Microgametogenesis F30 Plant genetics and breeding F63 Plant physiology - Reproduction F60 Plant physiology and biochemistry Q01 Food science and technology Citrus sinensis Clementines Tetraploids Carbohydrates Breeding Seedlessness is one of the most important agronomic traits in mandarins on the fresh fruit market. Creation of triploid plants is an important breeding strategy for development of new commercial varieties of seedless citrus. To this end, one strategy is to perform sexual hybridizations, with tetraploid genotypes as male parents. However, while seed development has been widely studied in citrus, knowledge of key steps such as microsporogenesis and microgametogenesis, is scarce, especially in polyploids. Therefore, we performed a study on the effect of ploidy level on pollen development by including diploid and tetraploid (double diploid) genotypes with different degrees of pollen performance. A comprehensive study on the pollen ontogeny of diploid and doubled diploid “Sanguinelli” blood orange and “Clemenules” clementine was performed, with focus on pollen grain germination in vitro and in planta, morphology of mature pollen grains by scanning electron microscopy (SEM), cytochemical characterization of carbohydrates by periodic acid–Shiff staining, and specific cell wall components revealed by immunolocalization. During microsporogenesis, the main difference between diploid and doubled diploid genotypes was cell area, which was larger in doubled diploid genotypes. However, after increase in size and vacuolization of microspores, but before mitosis I, doubled diploid “Clemenules” clementine showed drastic differences in shape, cell area, and starch hydrolysis, which resulted in shrinkage of pollen grains. The loss of fertility in doubled diploid “Clemenules” clementine is mainly due to lack of carbohydrate accumulation in pollen during microgametogenesis, especially starch content, which led to pollen grain abortion. All these changes make the pollen of this genotype unviable and very difficult to use as a male parent in sexual hybridization with the objective of recovering large progenies of triploid hybrids. 2022-06-03T08:50:09Z 2022-06-03T08:50:09Z 2022 article publishedVersion Lora, J., Garcia-Lor, A. & Aleza, P. (2022) Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species. Frontiers in Plant Science, 13, 862813. 1664-462X http://hdl.handle.net/20.500.11939/8188 10.3389/fpls.2022.862813 https://www.frontiersin.org/articles/10.3389/fpls.2022.862813/full en info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2015-00069-00-00 info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-109566RB-I00//UTILIZACION DE RECURSOS GENETICOS EN FRUTALES SUBTROPICALES MEDIANTE HERRAMIENTAS GENOMICAS Y DE BIOLOGIA REPRODUCTIVA This study was supported by grant RTA2015-00069-00-00 from the Ministry of “Economía y Competividad,” “Instituto Nacional de Investigación y Tecnología Agraria y Agroalimentaria,” and “Fondo Europeo de Desarrollo Regional” (FEDER). JL was supported by "Ministerio de Ciencia e Innovación”-European Regional Development Fund, European Union (PID-2019- 109566RB-100). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Frontiers Media electronico |
| spellingShingle | Pollen performance Microsporogenesis Microgametogenesis F30 Plant genetics and breeding F63 Plant physiology - Reproduction F60 Plant physiology and biochemistry Q01 Food science and technology Citrus sinensis Clementines Tetraploids Carbohydrates Breeding Lora, Jorge García-Lor, Andrés Aleza, Pablo Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title | Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title_full | Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title_fullStr | Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title_full_unstemmed | Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title_short | Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species |
| title_sort | pollen development and viability in diploid and doubled diploid citrus species |
| topic | Pollen performance Microsporogenesis Microgametogenesis F30 Plant genetics and breeding F63 Plant physiology - Reproduction F60 Plant physiology and biochemistry Q01 Food science and technology Citrus sinensis Clementines Tetraploids Carbohydrates Breeding |
| url | http://hdl.handle.net/20.500.11939/8188 https://www.frontiersin.org/articles/10.3389/fpls.2022.862813/full |
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