Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants
Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite, effective at controlling whiteflies and thrips in protected crops. However, on tomato its efficacy as a biocontrol agent is hindered, most probably by the plant trichomes and their exudates. Our aim was to characterize the...
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| Format: | Artículo |
| Language: | Inglés |
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Springer Nature
2022
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| Online Access: | http://hdl.handle.net/20.500.11939/8153 https://link.springer.com/article/10.1007/s10493-022-00715-w |
| _version_ | 1855492435519471616 |
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| author | Paspati, Angeliki Urbaneja, Alberto González-Cabrera, Joel |
| author_browse | González-Cabrera, Joel Paspati, Angeliki Urbaneja, Alberto |
| author_facet | Paspati, Angeliki Urbaneja, Alberto González-Cabrera, Joel |
| author_sort | Paspati, Angeliki |
| collection | ReDivia |
| description | Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite, effective at controlling whiteflies and thrips in protected crops. However, on tomato its efficacy as a biocontrol agent is hindered, most probably by the plant trichomes and their exudates. Our aim was to characterize the response of A. swirskii to the tomato trichome exudates and identify three major detoxification gene sets in this species: cytochromes P450 (CYPs), glutathione S-transferases (GSTs) and carboxyl/cholinesterases (CCEs). Mites were exposed separately to tomato and pepper, a favourable host plant for A. swirskii, after which their transcriptional responses were analysed and compared. The de novo transcriptome assembly resulted in 71,336 unigenes with 66.1% of them annotated. Thirty-nine A. swirskii genes were differentially expressed after transfer on tomato leaves when compared to pepper leaves; some of the expressed genes were associated with the metabolism of tomato exudates. Our results illustrate that the detoxification gene sets CYPs, GSTs and CCEs are abundant in A. swirskii, but do not play a significant role when in contact with the tomato exudates. |
| format | Artículo |
| id | ReDivia8153 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia81532025-04-25T14:48:47Z Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants Paspati, Angeliki Urbaneja, Alberto González-Cabrera, Joel Acyl sugar Differential expression Phytoseiid H10 Pests of plants U40 Surveying methods Detoxification RNA sequence Trichomes Tomatoes Exudates Pepper Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite, effective at controlling whiteflies and thrips in protected crops. However, on tomato its efficacy as a biocontrol agent is hindered, most probably by the plant trichomes and their exudates. Our aim was to characterize the response of A. swirskii to the tomato trichome exudates and identify three major detoxification gene sets in this species: cytochromes P450 (CYPs), glutathione S-transferases (GSTs) and carboxyl/cholinesterases (CCEs). Mites were exposed separately to tomato and pepper, a favourable host plant for A. swirskii, after which their transcriptional responses were analysed and compared. The de novo transcriptome assembly resulted in 71,336 unigenes with 66.1% of them annotated. Thirty-nine A. swirskii genes were differentially expressed after transfer on tomato leaves when compared to pepper leaves; some of the expressed genes were associated with the metabolism of tomato exudates. Our results illustrate that the detoxification gene sets CYPs, GSTs and CCEs are abundant in A. swirskii, but do not play a significant role when in contact with the tomato exudates. 2022-05-24T14:34:37Z 2022-05-24T14:34:37Z 2022 article publishedVersion Paspati, A., Urbaneja, A. & González-Cabrera, J. (2022). Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants. Experimental and Applied Acarology, 86, 479-498. 0168-8162 (print ISSN) 1572-9702 (eISSN) http://hdl.handle.net/20.500.11939/8153 10.1007/s10493-022-00715-w https://link.springer.com/article/10.1007/s10493-022-00715-w en info:eu-repo/grantAgreement/EC/H2020/641456 info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/RYC-2013-13834 This work was funded by the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No 641456. JGC was supported by the Spanish Ministry of Economy and Competitiveness, Ramón y Cajal Program (RYC-2013–13834). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer Nature electronico |
| spellingShingle | Acyl sugar Differential expression Phytoseiid H10 Pests of plants U40 Surveying methods Detoxification RNA sequence Trichomes Tomatoes Exudates Pepper Paspati, Angeliki Urbaneja, Alberto González-Cabrera, Joel Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title | Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title_full | Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title_fullStr | Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title_full_unstemmed | Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title_short | Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants |
| title_sort | transcriptomic profile of the predatory mite amblyseius swirskii acari phytoseiidae on different host plants |
| topic | Acyl sugar Differential expression Phytoseiid H10 Pests of plants U40 Surveying methods Detoxification RNA sequence Trichomes Tomatoes Exudates Pepper |
| url | http://hdl.handle.net/20.500.11939/8153 https://link.springer.com/article/10.1007/s10493-022-00715-w |
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