HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity
Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both cla...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8111 https://www.mdpi.com/1422-0067/23/9/5130 |
| _version_ | 1855032731211137024 |
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| author | Asins, María J. Romero-Aranda, María R. Espinosa, Jesús González-Fernández, Paloma Jaime-Fernández, Emilio Traverso, Jose A. Carbonell, Emilio A. Belver, Andres |
| author_browse | Asins, María J. Belver, Andres Carbonell, Emilio A. Espinosa, Jesús González-Fernández, Paloma Jaime-Fernández, Emilio Romero-Aranda, María R. Traverso, Jose A. |
| author_facet | Asins, María J. Romero-Aranda, María R. Espinosa, Jesús González-Fernández, Paloma Jaime-Fernández, Emilio Traverso, Jose A. Carbonell, Emilio A. Belver, Andres |
| author_sort | Asins, María J. |
| collection | ReDivia |
| description | Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both class I Na+ transporters HKT1;1 and HKT1;2 were used to investigate the silencing effects on the Na and K contents of the xylem sap, and source and sink organs of the scion, and their contribution to salt tolerance in all 16 rootstock/scion combinations of non-silenced and silenced lines, under two salinity treatments. The results show that SgHKT1;1 is operating differently from SgHKT1;2 regarding Na circulation in the tomato vascular system under salinity. A model was built to show that using silenced SgHKT1;1 line as rootstock would improve salt tolerance and fruit quality of varieties carrying the wild type SgHKT1;2 allele. Moreover, this increasing effect on both yield and fruit soluble solids content of silencing SgHKT1;1 could explain that a low expressing HKT1;1 variant was fixed in S. lycopersicum during domestication, and the paradox of increasing agronomic salt tolerance through silencing the HKT1;1 allele from S. galapagense, a salt adapted species. |
| format | article |
| id | ReDivia8111 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia81112025-04-25T14:48:45Z HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity Asins, María J. Romero-Aranda, María R. Espinosa, Jesús González-Fernández, Paloma Jaime-Fernández, Emilio Traverso, Jose A. Carbonell, Emilio A. Belver, Andres S. cheesmaniae Rootstock breeding Fruit quality K+ and Na+ homeostasis Long-distance transport F40 Plant ecology F61 Plant physiology - Nutrition F30 Plant genetics and breeding F60 Plant physiology and biochemistry Tomatoes Salt tolerance Yields Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both class I Na+ transporters HKT1;1 and HKT1;2 were used to investigate the silencing effects on the Na and K contents of the xylem sap, and source and sink organs of the scion, and their contribution to salt tolerance in all 16 rootstock/scion combinations of non-silenced and silenced lines, under two salinity treatments. The results show that SgHKT1;1 is operating differently from SgHKT1;2 regarding Na circulation in the tomato vascular system under salinity. A model was built to show that using silenced SgHKT1;1 line as rootstock would improve salt tolerance and fruit quality of varieties carrying the wild type SgHKT1;2 allele. Moreover, this increasing effect on both yield and fruit soluble solids content of silencing SgHKT1;1 could explain that a low expressing HKT1;1 variant was fixed in S. lycopersicum during domestication, and the paradox of increasing agronomic salt tolerance through silencing the HKT1;1 allele from S. galapagense, a salt adapted species. 2022-05-17T08:04:39Z 2022-05-17T08:04:39Z 2022 article publishedVersion Asins, M. J., Romero-Aranda, M. R., Espinosa, J., González-Fernández, P., Jaime-Fernández, E., Traverso, J. A. et al. (2022). HKT1; 1 and HKT1; 2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity. International Journal of Molecular Sciences, 23(9), 5130. 1422-0067 http://hdl.handle.net/20.500.11939/8111 10.3390/ijms23095130 https://www.mdpi.com/1422-0067/23/9/5130 en info:eu-repo/grantAgreement/AEI//AGL2017- 82452-C2 The study was funded by the Spanish Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, grants AGL2017-82452-C2-1R (A.B.) and AGL2017-82452-C2-2R (M.J.A) both from the, MCIN/AEI/10.13039/501100011033, the European Union (H2020 SFS-28-2020), contract number 101000716 (HARNESSTOM), and FEDER “Una manera de hacer Europa”. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | S. cheesmaniae Rootstock breeding Fruit quality K+ and Na+ homeostasis Long-distance transport F40 Plant ecology F61 Plant physiology - Nutrition F30 Plant genetics and breeding F60 Plant physiology and biochemistry Tomatoes Salt tolerance Yields Asins, María J. Romero-Aranda, María R. Espinosa, Jesús González-Fernández, Paloma Jaime-Fernández, Emilio Traverso, Jose A. Carbonell, Emilio A. Belver, Andres HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title | HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title_full | HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title_fullStr | HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title_full_unstemmed | HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title_short | HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity |
| title_sort | hkt1 1 and hkt1 2 na transporters from solanum galapagense play different roles in the plant na distribution under salinity |
| topic | S. cheesmaniae Rootstock breeding Fruit quality K+ and Na+ homeostasis Long-distance transport F40 Plant ecology F61 Plant physiology - Nutrition F30 Plant genetics and breeding F60 Plant physiology and biochemistry Tomatoes Salt tolerance Yields |
| url | http://hdl.handle.net/20.500.11939/8111 https://www.mdpi.com/1422-0067/23/9/5130 |
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