Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons
Changes in semen microbiota are associated with alterations to sperm quality and fertility. However, the microbiota from most livestock species has not yet been studied. Goats are seasonal breeders, but semen microbiota has never been described in this species, and it is unknown how seasonality affe...
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| Format: | article |
| Language: | Inglés |
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Frontiers Media
2022
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| Online Access: | http://hdl.handle.net/20.500.11939/8110 https://www.frontiersin.org/articles/10.3389/fvets.2022.867671/full |
| _version_ | 1855032731021344768 |
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| author | Mocé, Maria Lorena Esteve, Inés Carolina Pérez-Fuentes, Sara Gómez, Ernesto A. Mocé, Eva |
| author_browse | Esteve, Inés Carolina Gómez, Ernesto A. Mocé, Eva Mocé, Maria Lorena Pérez-Fuentes, Sara |
| author_facet | Mocé, Maria Lorena Esteve, Inés Carolina Pérez-Fuentes, Sara Gómez, Ernesto A. Mocé, Eva |
| author_sort | Mocé, Maria Lorena |
| collection | ReDivia |
| description | Changes in semen microbiota are associated with alterations to sperm quality and fertility. However, the microbiota from most livestock species has not yet been studied. Goats are seasonal breeders, but semen microbiota has never been described in this species, and it is unknown how seasonality affects it. Our study objective is 2-fold: to describe the microbiota in goat buck ejaculates and to determine if it differs between breeding and non-breeding seasons. Semen from six males of the Murciano-Granadina breed was collected during both seasons. Two replicates were performed per male and season on different days. The microbiota was characterized by genomic sequencing technology. Sperm quality was also evaluated. Repetition was not significant for the studied variables. Sperm velocities were higher for the breeding than for the non-breeding season. The ejaculates from both seasons also differed in the proportion of apoptotic spermatozoa. The five dominant phyla were Firmicutes, Proteobacteria, Fusobacteria, Actinobacteria, and Bacteroidetes during the breeding season and Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Cyanobacteria during the non-breeding season. The dominant genus during both seasons was Ureaplasma. Differences in microbial community structure (the beta diversity) were found. A decrease in the relative abundance of the genus Faecalibacterium and an increase in the genera Sphingomonas and Halomonas were observed in the ejaculates collected during the breeding season. Sphingomonas and Faecalibacterium abundance favorably and unfavorably correlated with sperm quality, respectively. In conclusion, the semen microbiota from goat bucks varies between breeding and nonbreeding seasons, and the microbiota remains stable for 7 days within a season. In addition, the genera Sphingomonas and Faecalibacterium could be possible biomarkers of semen quality in goat bucks. These results contribute to an in-depth understanding of the effects of reproductive seasonality on goat buck ejaculates. |
| format | article |
| id | ReDivia8110 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia81102025-04-25T14:48:45Z Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons Mocé, Maria Lorena Esteve, Inés Carolina Pérez-Fuentes, Sara Gómez, Ernesto A. Mocé, Eva Microbiota (16S) Microbiome Anestrous Reproductive season Caprine Sphingomonas Faecalibacterium Semen quality L10 Animal genetics and breeding U30 Research methods Sperm Semen Ejaculate Goats Changes in semen microbiota are associated with alterations to sperm quality and fertility. However, the microbiota from most livestock species has not yet been studied. Goats are seasonal breeders, but semen microbiota has never been described in this species, and it is unknown how seasonality affects it. Our study objective is 2-fold: to describe the microbiota in goat buck ejaculates and to determine if it differs between breeding and non-breeding seasons. Semen from six males of the Murciano-Granadina breed was collected during both seasons. Two replicates were performed per male and season on different days. The microbiota was characterized by genomic sequencing technology. Sperm quality was also evaluated. Repetition was not significant for the studied variables. Sperm velocities were higher for the breeding than for the non-breeding season. The ejaculates from both seasons also differed in the proportion of apoptotic spermatozoa. The five dominant phyla were Firmicutes, Proteobacteria, Fusobacteria, Actinobacteria, and Bacteroidetes during the breeding season and Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Cyanobacteria during the non-breeding season. The dominant genus during both seasons was Ureaplasma. Differences in microbial community structure (the beta diversity) were found. A decrease in the relative abundance of the genus Faecalibacterium and an increase in the genera Sphingomonas and Halomonas were observed in the ejaculates collected during the breeding season. Sphingomonas and Faecalibacterium abundance favorably and unfavorably correlated with sperm quality, respectively. In conclusion, the semen microbiota from goat bucks varies between breeding and nonbreeding seasons, and the microbiota remains stable for 7 days within a season. In addition, the genera Sphingomonas and Faecalibacterium could be possible biomarkers of semen quality in goat bucks. These results contribute to an in-depth understanding of the effects of reproductive seasonality on goat buck ejaculates. 2022-05-13T08:01:23Z 2022-05-13T08:01:23Z 2022 article publishedVersion Mocé, M. L., Esteve, I. C., Pérez-Fuentes, S., Gómez, E. A. & Mocé, E. (2022). Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons. Frontiers in Veterinary Science, 9, 867671. 2297-1769 http://hdl.handle.net/20.500.11939/8110 10.3389/fvets.2022.867671 https://www.frontiersin.org/articles/10.3389/fvets.2022.867671/full en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTA2017-00049-C02-01 info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51906 info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201K This work was supported by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and was cofinanced by ERDF (Grant No. RTA2017-00049-C02-01), GVAIVIA and was co-funded by the EU through the Operational Programmes ERDF of the Comunitat Valenciana 2014-2020 and 2021-2027 (Grant Nos. 51906 and 52201K), AMURVAL (Contract No. 71714), and by the Universidad Cardenal Herrera- CEU, CEU Universities (Grant Nos. INDI20/34 and INDI21/40). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Frontiers Media electronico |
| spellingShingle | Microbiota (16S) Microbiome Anestrous Reproductive season Caprine Sphingomonas Faecalibacterium Semen quality L10 Animal genetics and breeding U30 Research methods Sperm Semen Ejaculate Goats Mocé, Maria Lorena Esteve, Inés Carolina Pérez-Fuentes, Sara Gómez, Ernesto A. Mocé, Eva Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title | Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title_full | Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title_fullStr | Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title_full_unstemmed | Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title_short | Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons |
| title_sort | microbiota in goat buck ejaculates differs between breeding and non breeding seasons |
| topic | Microbiota (16S) Microbiome Anestrous Reproductive season Caprine Sphingomonas Faecalibacterium Semen quality L10 Animal genetics and breeding U30 Research methods Sperm Semen Ejaculate Goats |
| url | http://hdl.handle.net/20.500.11939/8110 https://www.frontiersin.org/articles/10.3389/fvets.2022.867671/full |
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