Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles
Two types of commercial Na-zeolites, linde type A (LTA) and faujasite (FAU) were prepared with different percentage of silver that was incorporated by ion exchange using solutions of AgNO3. The physico-chemical properties of the samples were established using different techniques such as atomic emis...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Objeto de conferencia |
| Language: | Inglés |
| Published: |
ISHS
2022
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/8108 https://www.actahort.org/books/1323/1323_10.htm |
| _version_ | 1855492427144495104 |
|---|---|
| author | Palou, Lluís Cerrillo, José L. Klangmuang, P. Pérez-Gago, María B. Taberner, Verònica De-La-Fuente, Beatriz Sothornvit, Rungsinee Rey, Fernando Valencia, Susana Palomares, Antonio E. |
| author2 | Korsten, L. |
| author_browse | Cerrillo, José L. De-La-Fuente, Beatriz Klangmuang, P. Korsten, L. Palomares, Antonio E. Palou, Lluís Pérez-Gago, María B. Rey, Fernando Sothornvit, Rungsinee Taberner, Verònica Valencia, Susana |
| author_facet | Korsten, L. Palou, Lluís Cerrillo, José L. Klangmuang, P. Pérez-Gago, María B. Taberner, Verònica De-La-Fuente, Beatriz Sothornvit, Rungsinee Rey, Fernando Valencia, Susana Palomares, Antonio E. |
| author_sort | Palou, Lluís |
| collection | ReDivia |
| description | Two types of commercial Na-zeolites, linde type A (LTA) and faujasite (FAU) were prepared with different percentage of silver that was incorporated by ion exchange using solutions of AgNO3. The physico-chemical properties of the samples were established using different techniques such as atomic emission spectroscopy with inductively coupled plasma (ICP-OES), X-ray diffraction (XRD), electronic microscopy (SEM and EDX), and temperature-programmed reduction (TPR). The curative activity of these materials was first evaluated as water dispersions in in vivo primary screenings by placing a drop (30 µL) in a rind wound of 'Valencia' oranges that had been inoculated 24 h before with a conidial suspension of Penicillium digitatum. Selected Ag-zeolites were incorporated as antifungal ingredients at different concentrations into composite hydroxypropyl methylcellulose (HPMC)-beeswax (BW) edible emulsions prepared with 8% total solid content with stearic acid as emulsifier and glycerol as plasticizer. Depending on the experiment, these coatings were applied 24 h after fungal inoculation to 'Valencia' oranges as drops in rind wounds or covering the entire fruit. Controls included inoculated but untreated fruit and inoculated fruit treated with HPMC-BW without Ag-zeolites. After incubation of treated fruit at 20°C for 7 days, coatings amended with Ag-zeolites reduced the incidence and severity of green mold by more than 80%, showing high potential for effective disease control. Curative activity depended on Ag concentration and FAU zeolites were more effective than LTA zeolites, probably because of their larger pore apertures and higher Si/Al ratio that favored silver diffusion. However, coatings with the highest silver content were phytotoxic causing dark blemishes on the fruit rind. Further work is needed to optimize non-phytotoxic coatings with the highest curative activity. |
| format | Objeto de conferencia |
| id | ReDivia8108 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | ISHS |
| publisherStr | ISHS |
| record_format | dspace |
| spelling | ReDivia81082025-04-25T14:53:00Z Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles Palou, Lluís Cerrillo, José L. Klangmuang, P. Pérez-Gago, María B. Taberner, Verònica De-La-Fuente, Beatriz Sothornvit, Rungsinee Rey, Fernando Valencia, Susana Palomares, Antonio E. Korsten, L. Alternative postharvest disease control Nanotechnology GRAS salts H20 Plant diseases J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q03 Food contamination and toxicology U30 Research methods Penicillium digitatum Edible coatings Health foods Antifungal properties Zeolites Postharvest treatment Two types of commercial Na-zeolites, linde type A (LTA) and faujasite (FAU) were prepared with different percentage of silver that was incorporated by ion exchange using solutions of AgNO3. The physico-chemical properties of the samples were established using different techniques such as atomic emission spectroscopy with inductively coupled plasma (ICP-OES), X-ray diffraction (XRD), electronic microscopy (SEM and EDX), and temperature-programmed reduction (TPR). The curative activity of these materials was first evaluated as water dispersions in in vivo primary screenings by placing a drop (30 µL) in a rind wound of 'Valencia' oranges that had been inoculated 24 h before with a conidial suspension of Penicillium digitatum. Selected Ag-zeolites were incorporated as antifungal ingredients at different concentrations into composite hydroxypropyl methylcellulose (HPMC)-beeswax (BW) edible emulsions prepared with 8% total solid content with stearic acid as emulsifier and glycerol as plasticizer. Depending on the experiment, these coatings were applied 24 h after fungal inoculation to 'Valencia' oranges as drops in rind wounds or covering the entire fruit. Controls included inoculated but untreated fruit and inoculated fruit treated with HPMC-BW without Ag-zeolites. After incubation of treated fruit at 20°C for 7 days, coatings amended with Ag-zeolites reduced the incidence and severity of green mold by more than 80%, showing high potential for effective disease control. Curative activity depended on Ag concentration and FAU zeolites were more effective than LTA zeolites, probably because of their larger pore apertures and higher Si/Al ratio that favored silver diffusion. However, coatings with the highest silver content were phytotoxic causing dark blemishes on the fruit rind. Further work is needed to optimize non-phytotoxic coatings with the highest curative activity. 2022-05-13T07:56:15Z 2022-05-13T07:56:15Z 2021 conferenceObject submittedVersion Palou, L., Cerrillo, J. L., Klangmuang, P., Pérez-Gago, M. B., Taberner, V., de-la-Fuente, B. et al. (2021). Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles. Acta Hortic. 1323, 59-64 978-94-62613-21-8 0567-7572 (print) 2406-6168 (electronic) http://hdl.handle.net/20.500.11939/8108 10.17660/ActaHortic.2021.1323.10 https://www.actahort.org/books/1323/1323_10.htm en 2017-05-28 IV International Symposium on Postharvest Pathology: Next Generation Innovation and Commercial Solutions for Postharvest Pathology to Reduce Losses, Enhance Quality, and Ensure Product Safety Skukuza (South Africa) Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess ISHS electronico |
| spellingShingle | Alternative postharvest disease control Nanotechnology GRAS salts H20 Plant diseases J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q03 Food contamination and toxicology U30 Research methods Penicillium digitatum Edible coatings Health foods Antifungal properties Zeolites Postharvest treatment Palou, Lluís Cerrillo, José L. Klangmuang, P. Pérez-Gago, María B. Taberner, Verònica De-La-Fuente, Beatriz Sothornvit, Rungsinee Rey, Fernando Valencia, Susana Palomares, Antonio E. Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title | Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title_full | Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title_fullStr | Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title_full_unstemmed | Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title_short | Curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose-beeswax edible coatings with zeolites containing Ag-nanoparticles |
| title_sort | curative activity against citrus postharvest green mold of composite hydroxypropyl methylcellulose beeswax edible coatings with zeolites containing ag nanoparticles |
| topic | Alternative postharvest disease control Nanotechnology GRAS salts H20 Plant diseases J10 Handling, transport, storage and protection of agricultural products Q02 Food processing and preservation Q03 Food contamination and toxicology U30 Research methods Penicillium digitatum Edible coatings Health foods Antifungal properties Zeolites Postharvest treatment |
| url | http://hdl.handle.net/20.500.11939/8108 https://www.actahort.org/books/1323/1323_10.htm |
| work_keys_str_mv | AT paloulluis curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT cerrillojosel curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT klangmuangp curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT perezgagomariab curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT tabernerveronica curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT delafuentebeatriz curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT sothornvitrungsinee curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT reyfernando curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT valenciasusana curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles AT palomaresantonioe curativeactivityagainstcitruspostharvestgreenmoldofcompositehydroxypropylmethylcellulosebeeswaxediblecoatingswithzeolitescontainingagnanoparticles |