Improving Therapy Methods for Citrus Germplasm Exchange
Movement of citrus germplasm between citrus-growing countries carries the risk of inadvertent spread of serious virus and viruslike pathogens. Current procedures have not been well tested for all pathogens, or are not completely efficient. The exclusion of the two pathovars of Xanthomonas campestris...
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| Format: | conferenceObject |
| Language: | Inglés |
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IOCV
2022
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| Online Access: | http://hdl.handle.net/20.500.11939/8093 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th400_408.pdf |
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| author | Navarro, Luis Civerolo, E. L. Juárez, José Garnsey, Stephen M. |
| author2 | Brlansky, R. H. |
| author_browse | Brlansky, R. H. Civerolo, E. L. Garnsey, Stephen M. Juárez, José Navarro, Luis |
| author_facet | Brlansky, R. H. Navarro, Luis Civerolo, E. L. Juárez, José Garnsey, Stephen M. |
| author_sort | Navarro, Luis |
| collection | ReDivia |
| description | Movement of citrus germplasm between citrus-growing countries carries the risk of inadvertent spread of serious virus and viruslike pathogens. Current procedures have not been well tested for all pathogens, or are not completely efficient. The exclusion of the two pathovars of Xanthomonas campestris causing citrus canker and citrus bacterial spot, the greening pathogen, severe isolates of citrus tristeza virus (CTV) and citrus tatterleaf virus (TLV) was studied using a tissue culture quarantine methodology. Infected budsticks were cultured in vitro at 32 C. Shoot tips excised from sprouting buds were grafted in vitro to axenically grown seedlings of Troyer citrange. The citrus canker and the greening pathogens were consistently eliminated, even by grafting large shoot tips of
0.5-0.7 mm. Severe strains of CTV were consistently eliminated only by the use of small shoot tips of 0.15-0.2 mm. TLV was difficult to eliminate, but 42% of the plants propagated from shoot tips with three-leaf primordia were TLV-free. Shoot-tip grafting eliminated greening and CTV from chronically infected plants from Africa and Asia. Severe CTV isolates from Africa, Asia, South America, and North America were also eliminated easily. One isolate of naturally-spreading psorosis from Argentina was
also eliminated. |
| format | conferenceObject |
| id | ReDivia8093 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia80932025-04-25T14:51:47Z Improving Therapy Methods for Citrus Germplasm Exchange Navarro, Luis Civerolo, E. L. Juárez, José Garnsey, Stephen M. Brlansky, R. H. J10 Handling, transport, storage and protection of agricultural products H20 Plant diseases U30 Research methods E71 International trade Germplasm Citrus Plant viruses Tissue culture Quarantine Xanthomonas campestris Citrus tristeza virus Biological safety Thermotherapy Movement of citrus germplasm between citrus-growing countries carries the risk of inadvertent spread of serious virus and viruslike pathogens. Current procedures have not been well tested for all pathogens, or are not completely efficient. The exclusion of the two pathovars of Xanthomonas campestris causing citrus canker and citrus bacterial spot, the greening pathogen, severe isolates of citrus tristeza virus (CTV) and citrus tatterleaf virus (TLV) was studied using a tissue culture quarantine methodology. Infected budsticks were cultured in vitro at 32 C. Shoot tips excised from sprouting buds were grafted in vitro to axenically grown seedlings of Troyer citrange. The citrus canker and the greening pathogens were consistently eliminated, even by grafting large shoot tips of 0.5-0.7 mm. Severe strains of CTV were consistently eliminated only by the use of small shoot tips of 0.15-0.2 mm. TLV was difficult to eliminate, but 42% of the plants propagated from shoot tips with three-leaf primordia were TLV-free. Shoot-tip grafting eliminated greening and CTV from chronically infected plants from Africa and Asia. Severe CTV isolates from Africa, Asia, South America, and North America were also eliminated easily. One isolate of naturally-spreading psorosis from Argentina was also eliminated. 2022-05-10T09:02:37Z 2022-05-10T09:02:37Z 1991 conferenceObject Navarro, L., Civerolo, E. L., Juarez, J. & Garnsey, S. M. (1991). Improving therapy methods for citrus germplasm exchange. Proceedings of the 11th Conference of the IOCV, 400-408. 2313-5123 http://hdl.handle.net/20.500.11939/8093 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th400_408.pdf en 1991 Eleventh Conference of the International Organization of Citrus Virologists Orlando, Florida This work was carried out at the USDA quarantine facilities at Beltsville, Maryland, USA, and it was financed by a grant from the International Board of Plant Genetic Resources. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | J10 Handling, transport, storage and protection of agricultural products H20 Plant diseases U30 Research methods E71 International trade Germplasm Citrus Plant viruses Tissue culture Quarantine Xanthomonas campestris Citrus tristeza virus Biological safety Thermotherapy Navarro, Luis Civerolo, E. L. Juárez, José Garnsey, Stephen M. Improving Therapy Methods for Citrus Germplasm Exchange |
| title | Improving Therapy Methods for Citrus Germplasm Exchange |
| title_full | Improving Therapy Methods for Citrus Germplasm Exchange |
| title_fullStr | Improving Therapy Methods for Citrus Germplasm Exchange |
| title_full_unstemmed | Improving Therapy Methods for Citrus Germplasm Exchange |
| title_short | Improving Therapy Methods for Citrus Germplasm Exchange |
| title_sort | improving therapy methods for citrus germplasm exchange |
| topic | J10 Handling, transport, storage and protection of agricultural products H20 Plant diseases U30 Research methods E71 International trade Germplasm Citrus Plant viruses Tissue culture Quarantine Xanthomonas campestris Citrus tristeza virus Biological safety Thermotherapy |
| url | http://hdl.handle.net/20.500.11939/8093 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th400_408.pdf |
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