An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29
An atypical strain of Erwinia amylovora was isolated near an outbreak of fire blight at a nursery in Spain in 1996. It was obtained from a Crataegus plant showing typical symptoms and was identified as E. amy-lovora by biochemical tests and enrichment-enzyme-linked immuno-sorbent assay, but not by p...
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| Format: | article |
| Language: | Inglés |
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American Phytopathological Society (APS)
2022
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| Online Access: | http://hdl.handle.net/20.500.11939/8058 https://apsjournals.apsnet.org/doi/epdfplus/10.1094/PHYTO-96-0900 |
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| author | Llop, Pablo Donat, Victoria Rodriguez, Margarita Cabrefiga, Jordi Ruz, Lidia Palomares, Antonio E. Montesinos, Emilio López, María M. |
| author_browse | Cabrefiga, Jordi Donat, Victoria Llop, Pablo López, María M. Montesinos, Emilio Palomares, Antonio E. Rodriguez, Margarita Ruz, Lidia |
| author_facet | Llop, Pablo Donat, Victoria Rodriguez, Margarita Cabrefiga, Jordi Ruz, Lidia Palomares, Antonio E. Montesinos, Emilio López, María M. |
| author_sort | Llop, Pablo |
| collection | ReDivia |
| description | An atypical strain of Erwinia amylovora was isolated near an outbreak of fire blight at a nursery in Spain in 1996. It was obtained from a Crataegus plant showing typical symptoms and was identified as E. amy-lovora by biochemical tests and enrichment-enzyme-linked immuno-sorbent assay, but not by polymerase chain reaction using primers based on the pEA29 sequence. Nevertheless, with primers from chromosomal regions, the isolate gave the expected amplification band. This strain carries one plasmid of ≈70 kb, with no homology with the 29-kb plasmid common to all pathogenic strains, or with a large plasmid present in some E. amylovora strains. Growth of the strain in minimal medium without thiamine was slower compared with cultures in the same medium with thiamine, a characteristic typical of strains cured of the 29-kb plasmid. Nevertheless, aggressiveness assays on pear, apple, and Pyracantha plants and in immature pear fruit showed that this strain exhibited a virulence level similar to other strains containing pEA29. To the best of our knowledge, this is the first report of the isolation from naturally infected plant material of a pathogenic strain of E. amylovora without pEA29, but with a plasmid of ≈70 kb not previously described. |
| format | article |
| id | ReDivia8058 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Phytopathological Society (APS) |
| publisherStr | American Phytopathological Society (APS) |
| record_format | dspace |
| spelling | ReDivia80582025-04-25T14:48:42Z An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 Llop, Pablo Donat, Victoria Rodriguez, Margarita Cabrefiga, Jordi Ruz, Lidia Palomares, Antonio E. Montesinos, Emilio López, María M. Phenotypic characterization Strain comparison H20 Plant diseases U30 Research methods Erwinia amylovora Fire blight Virulence Plasmids An atypical strain of Erwinia amylovora was isolated near an outbreak of fire blight at a nursery in Spain in 1996. It was obtained from a Crataegus plant showing typical symptoms and was identified as E. amy-lovora by biochemical tests and enrichment-enzyme-linked immuno-sorbent assay, but not by polymerase chain reaction using primers based on the pEA29 sequence. Nevertheless, with primers from chromosomal regions, the isolate gave the expected amplification band. This strain carries one plasmid of ≈70 kb, with no homology with the 29-kb plasmid common to all pathogenic strains, or with a large plasmid present in some E. amylovora strains. Growth of the strain in minimal medium without thiamine was slower compared with cultures in the same medium with thiamine, a characteristic typical of strains cured of the 29-kb plasmid. Nevertheless, aggressiveness assays on pear, apple, and Pyracantha plants and in immature pear fruit showed that this strain exhibited a virulence level similar to other strains containing pEA29. To the best of our knowledge, this is the first report of the isolation from naturally infected plant material of a pathogenic strain of E. amylovora without pEA29, but with a plasmid of ≈70 kb not previously described. 2022-04-29T16:15:54Z 2022-04-29T16:15:54Z 2006 article publishedVersion Llop, P., Donat, V., Rodríguez, M., Cabrefiga, J., Ruz, L., Palomo, J. L. et al. (2006). An indigenous virulent strain of Erwinia amylovora lacking the ubiquitous plasmid pEA29. Phytopathology, 96(8), 900-907. 0031-949X 1943-7684 (e-ISSN) http://hdl.handle.net/20.500.11939/8058 10.1094/PHYTO-96-0900 https://apsjournals.apsnet.org/doi/epdfplus/10.1094/PHYTO-96-0900 en info:eu-repo/grantAgreement/CICYT//AGF98-0402-C03 info:eu-repo/grantAgreement/CICYT//AGL2001-2349-C03 This work was supported by Projects AGF98-0402-C03 and AGL2001-2349-C03 from the “Comisión Interministerial de Ciencia y Tecnología-CICYT” of Spain. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess American Phytopathological Society (APS) electronico |
| spellingShingle | Phenotypic characterization Strain comparison H20 Plant diseases U30 Research methods Erwinia amylovora Fire blight Virulence Plasmids Llop, Pablo Donat, Victoria Rodriguez, Margarita Cabrefiga, Jordi Ruz, Lidia Palomares, Antonio E. Montesinos, Emilio López, María M. An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title | An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title_full | An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title_fullStr | An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title_full_unstemmed | An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title_short | An Indigenous Virulent Strain of Erwinia amylovora Lacking the Ubiquitous Plasmid pEA29 |
| title_sort | indigenous virulent strain of erwinia amylovora lacking the ubiquitous plasmid pea29 |
| topic | Phenotypic characterization Strain comparison H20 Plant diseases U30 Research methods Erwinia amylovora Fire blight Virulence Plasmids |
| url | http://hdl.handle.net/20.500.11939/8058 https://apsjournals.apsnet.org/doi/epdfplus/10.1094/PHYTO-96-0900 |
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