Development of a Highly Sensitive Nested-PCR Procedure Using a Single Closed Tube for Detection of Erwinia amylovora in Asymptomatic Plant Material
A novel method, which involves a nested PCR in a single closed tube, was developed for the sensitive detection of Erwinia amylovora in plant material. The external and internal primer pairs used had different annealing temperatures and directed the amplification of a specific DNA fragment from plasm...
| Main Authors: | , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
American Society for Microbiology ("ASM")
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/8057 https://journals.asm.org/doi/epub/10.1128/AEM.66.5.2071-2078.2000 |
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