A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours
A simple PCR protocol was developed for identifying Agrobacterium as the causal agent of the tumours produced by this bacterium in plant material. The sensitivity of this method was compared with that of bacterial isolation using common and selective media with a previous enrichment step. More than...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Society for Applied Microbiology
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8056 https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1046/j.1365-2672.1999.00700.x |
| _version_ | 1855032721219256320 |
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| author | Cubero, Jaime Martínez, M. Carmen Llop, Pablo López, María M. |
| author_browse | Cubero, Jaime Llop, Pablo López, María M. Martínez, M. Carmen |
| author_facet | Cubero, Jaime Martínez, M. Carmen Llop, Pablo López, María M. |
| author_sort | Cubero, Jaime |
| collection | ReDivia |
| description | A simple PCR protocol was developed for identifying Agrobacterium as the causal agent of the tumours produced by this bacterium in plant material. The sensitivity of this method was compared with that of bacterial isolation using common and selective media with a previous enrichment step. More than 200 samples from tumours of naturally infected and inoculated plants from several hosts including almond, peach×almond hybrids, apricot, rose, tobacco, tomato, raspberry, grapevine and chrysanthemum, were analysed by both methods. PCR was the most efficient method for detecting the bacterial aetiology of the plant tumours. Agrobacterium tumefaciens was better detected in crown and root tumours than in aerial tumours with all the methods assayed in inoculated plants. A comparison between the efficiency of the diagnosis by analysing pieces from the external and internal part of the tumour showed no differences between them. |
| format | article |
| id | ReDivia8056 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Society for Applied Microbiology |
| publisherStr | Society for Applied Microbiology |
| record_format | dspace |
| spelling | ReDivia80562025-04-25T14:48:42Z A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours Cubero, Jaime Martínez, M. Carmen Llop, Pablo López, María M. H20 Plant diseases U30 Research methods PCR Agrobacterium Diagnostic techniques Plant tumours A simple PCR protocol was developed for identifying Agrobacterium as the causal agent of the tumours produced by this bacterium in plant material. The sensitivity of this method was compared with that of bacterial isolation using common and selective media with a previous enrichment step. More than 200 samples from tumours of naturally infected and inoculated plants from several hosts including almond, peach×almond hybrids, apricot, rose, tobacco, tomato, raspberry, grapevine and chrysanthemum, were analysed by both methods. PCR was the most efficient method for detecting the bacterial aetiology of the plant tumours. Agrobacterium tumefaciens was better detected in crown and root tumours than in aerial tumours with all the methods assayed in inoculated plants. A comparison between the efficiency of the diagnosis by analysing pieces from the external and internal part of the tumour showed no differences between them. 2022-04-29T16:12:58Z 2022-04-29T16:12:58Z 2001 article publishedVersion Cubero, J., Martinez, M. C., Llop, P. & López, M. M. (1999). A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours. Journal of Applied Microbiology, 86(4), 591-602. 1365-2389 (eISSN) http://hdl.handle.net/20.500.11939/8056 10.1046/j.1365-2672.1999.00700.x https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1046/j.1365-2672.1999.00700.x en This research was grant funded by projects 93–117 of the INIA and by contract IC18-CT97–0198 of the INCO-DC programme of the European Community. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Society for Applied Microbiology electronico |
| spellingShingle | H20 Plant diseases U30 Research methods PCR Agrobacterium Diagnostic techniques Plant tumours Cubero, Jaime Martínez, M. Carmen Llop, Pablo López, María M. A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title | A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title_full | A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title_fullStr | A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title_full_unstemmed | A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title_short | A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours |
| title_sort | simple and efficient pcr method for the detection of agrobacterium tumefaciens in plant tumours |
| topic | H20 Plant diseases U30 Research methods PCR Agrobacterium Diagnostic techniques Plant tumours |
| url | http://hdl.handle.net/20.500.11939/8056 https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1046/j.1365-2672.1999.00700.x |
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