Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers
A PCR-based kit, ProbeliaTM, for the detection of Erwinia carotovora subsp.atroseptica (Eca) on potatoes was evaluated at five laboratories in four countries. The kit is based on DNA-specific PCR amplification followed by detection of amplicons by hybridization to a peroxidase-labelled DNA probe in...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8055 https://link.springer.com/article/10.1007/BF02358439 |
| _version_ | 1855032721034706944 |
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| author | Frechon, Dominique Exbrayat, Pascale Helias, Valerie Hyman, Lizbeth J. Jouan, B. Llop, Pablo López, María M. Payet, Nicole Perombelon, M. C. M. Toth, I. K. Van Beckhoven, Jose R. C. M. Van der Wolf, J. M. Bertheau, Y. |
| author_browse | Bertheau, Y. Exbrayat, Pascale Frechon, Dominique Helias, Valerie Hyman, Lizbeth J. Jouan, B. Llop, Pablo López, María M. Payet, Nicole Perombelon, M. C. M. Toth, I. K. Van Beckhoven, Jose R. C. M. Van der Wolf, J. M. |
| author_facet | Frechon, Dominique Exbrayat, Pascale Helias, Valerie Hyman, Lizbeth J. Jouan, B. Llop, Pablo López, María M. Payet, Nicole Perombelon, M. C. M. Toth, I. K. Van Beckhoven, Jose R. C. M. Van der Wolf, J. M. Bertheau, Y. |
| author_sort | Frechon, Dominique |
| collection | ReDivia |
| description | A PCR-based kit, ProbeliaTM, for the detection of Erwinia carotovora subsp.atroseptica (Eca) on potatoes was evaluated at five laboratories in four countries. The kit is based on DNA-specific PCR amplification followed by detection of amplicons by hybridization to a peroxidase-labelled DNA probe in a microplate. Specificity of the PCR primers for Eca, regardless of serogroups, was confirmed by testing against 246 bacterial, fungal and plant species. Detection limits of the assay varied little between six Eca strains in pure cultures (1.3×102 to 1.5×103 cells ml−1). When Eca-free tuber peel extract from four cultivars was inoculated with known numbers of 15 Eca strains, detection limits were more variable (1.0×101 to 6.2×103 cells ml−1 peel extract), attributed probably to inconsistency in the recovery of DNA during extraction. When the PCR assay was compared with three current commercial Eca detection methods, using naturally contaminated tubers, results matched most closely those from viable counts on a selective medium, the most sensitive method (88%), followed by enrichment ELISA (72%) and last ELISA (30%), the least sensitive method. |
| format | article |
| id | ReDivia8055 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia80552025-04-25T14:48:40Z Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers Frechon, Dominique Exbrayat, Pascale Helias, Valerie Hyman, Lizbeth J. Jouan, B. Llop, Pablo López, María M. Payet, Nicole Perombelon, M. C. M. Toth, I. K. Van Beckhoven, Jose R. C. M. Van der Wolf, J. M. Bertheau, Y. H20 Plant diseases ELISA Blackleg DNA Erwinia carotovora Potatoes A PCR-based kit, ProbeliaTM, for the detection of Erwinia carotovora subsp.atroseptica (Eca) on potatoes was evaluated at five laboratories in four countries. The kit is based on DNA-specific PCR amplification followed by detection of amplicons by hybridization to a peroxidase-labelled DNA probe in a microplate. Specificity of the PCR primers for Eca, regardless of serogroups, was confirmed by testing against 246 bacterial, fungal and plant species. Detection limits of the assay varied little between six Eca strains in pure cultures (1.3×102 to 1.5×103 cells ml−1). When Eca-free tuber peel extract from four cultivars was inoculated with known numbers of 15 Eca strains, detection limits were more variable (1.0×101 to 6.2×103 cells ml−1 peel extract), attributed probably to inconsistency in the recovery of DNA during extraction. When the PCR assay was compared with three current commercial Eca detection methods, using naturally contaminated tubers, results matched most closely those from viable counts on a selective medium, the most sensitive method (88%), followed by enrichment ELISA (72%) and last ELISA (30%), the least sensitive method. 2022-04-29T16:12:45Z 2022-04-29T16:12:45Z 1998 article publishedVersion Frechon, D., Exbrayat, P., Helias, V., Hyman, L. J., Jouan, B., Llop, P. et al.(1998). Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers. Potato research, 41(2), 163-173. 0014-3065 http://hdl.handle.net/20.500.11939/8055 10.1007/BF02358439 https://link.springer.com/article/10.1007/BF02358439 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Springer electronico |
| spellingShingle | H20 Plant diseases ELISA Blackleg DNA Erwinia carotovora Potatoes Frechon, Dominique Exbrayat, Pascale Helias, Valerie Hyman, Lizbeth J. Jouan, B. Llop, Pablo López, María M. Payet, Nicole Perombelon, M. C. M. Toth, I. K. Van Beckhoven, Jose R. C. M. Van der Wolf, J. M. Bertheau, Y. Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title | Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title_full | Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title_fullStr | Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title_full_unstemmed | Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title_short | Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers |
| title_sort | evaluation of a pcr kit for the detection of erwinia carotovora subsp atroseptica on potato tubers |
| topic | H20 Plant diseases ELISA Blackleg DNA Erwinia carotovora Potatoes |
| url | http://hdl.handle.net/20.500.11939/8055 https://link.springer.com/article/10.1007/BF02358439 |
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