Towards a phylogeny of the clostridia based on rRNA sequences
The 16S rRNA sequences of 17 species of the genus Clostridium were determined by direct sequencing of their PCR amplified genes. The sequences were aligned with those from other known clostridial species and representative low G + C Gram-positive relatives, and a phylogenetic tree was constructed. I...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Oxford University Press
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/8054 https://academic.oup.com/femsle/article/113/1/87/678293?login=true |
| _version_ | 1855492415651053568 |
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| author | Lawson, P. A. Llop, Pablo Hutson, R. A. Hippe, H. Collins, M. D. |
| author_browse | Collins, M. D. Hippe, H. Hutson, R. A. Lawson, P. A. Llop, Pablo |
| author_facet | Lawson, P. A. Llop, Pablo Hutson, R. A. Hippe, H. Collins, M. D. |
| author_sort | Lawson, P. A. |
| collection | ReDivia |
| description | The 16S rRNA sequences of 17 species of the genus Clostridium were determined by direct sequencing of their PCR amplified genes. The sequences were aligned with those from other known clostridial species and representative low G + C Gram-positive relatives, and a phylogenetic tree was constructed. It was evident from the comparative sequence analysis that the genus Clostridium as presently constituted is phylogenetically extremely heterogeneous. This study corroborates and extends earlier findings in showing that many non-sporeforming bacteria are phylogenetically closely intermixed with Clostridium species. The taxonomic implications of the phylogenetic findings are discussed. |
| format | Artículo |
| id | ReDivia8054 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia80542025-04-25T14:48:42Z Towards a phylogeny of the clostridia based on rRNA sequences Lawson, P. A. Llop, Pablo Hutson, R. A. Hippe, H. Collins, M. D. 165 r-DNA Molecular systematics H20 Plant diseases U30 Research methods Clostridium Phylogeny PCR The 16S rRNA sequences of 17 species of the genus Clostridium were determined by direct sequencing of their PCR amplified genes. The sequences were aligned with those from other known clostridial species and representative low G + C Gram-positive relatives, and a phylogenetic tree was constructed. It was evident from the comparative sequence analysis that the genus Clostridium as presently constituted is phylogenetically extremely heterogeneous. This study corroborates and extends earlier findings in showing that many non-sporeforming bacteria are phylogenetically closely intermixed with Clostridium species. The taxonomic implications of the phylogenetic findings are discussed. 2022-04-29T16:12:31Z 2022-04-29T16:12:31Z 1993 article publishedVersion Lawson, P. A., Llop, P., Hutson, R. A., Hippe, H. & Collins, M. D. (1993). Towards a phylogeny of the clostridia based on 16S rRNA sequences. FEMS microbiology letters, 113(1), 87-92. 0378-1097 1574-6968 (eISSN) http://hdl.handle.net/20.500.11939/8054 10.1111/j.1574-6968.1993.tb06493.x https://academic.oup.com/femsle/article/113/1/87/678293?login=true en This work was supported by grants from the Ministry of Agriculture, Fisheries and Food, and the EEC (HRAMI project, BIOT-CT91-0294 SSMA). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Oxford University Press electronico |
| spellingShingle | 165 r-DNA Molecular systematics H20 Plant diseases U30 Research methods Clostridium Phylogeny PCR Lawson, P. A. Llop, Pablo Hutson, R. A. Hippe, H. Collins, M. D. Towards a phylogeny of the clostridia based on rRNA sequences |
| title | Towards a phylogeny of the clostridia based on rRNA sequences |
| title_full | Towards a phylogeny of the clostridia based on rRNA sequences |
| title_fullStr | Towards a phylogeny of the clostridia based on rRNA sequences |
| title_full_unstemmed | Towards a phylogeny of the clostridia based on rRNA sequences |
| title_short | Towards a phylogeny of the clostridia based on rRNA sequences |
| title_sort | towards a phylogeny of the clostridia based on rrna sequences |
| topic | 165 r-DNA Molecular systematics H20 Plant diseases U30 Research methods Clostridium Phylogeny PCR |
| url | http://hdl.handle.net/20.500.11939/8054 https://academic.oup.com/femsle/article/113/1/87/678293?login=true |
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