Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora
Control of bacterial plant diseases is a major concern, as they affect economically important species and spread easily, such as the case of fire blight of rosaceous caused by Erwinia amylovora. In the search for alternatives to the use of agrochemicals and antibiotics, this work presents a screenin...
| Autores principales: | , , , , , , |
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| Formato: | acceptedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Society for Applied Microbiology and John Wiley & Sons Ltd.
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8053 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13064 |
| _version_ | 1855032720670851072 |
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| author | Barbé, Silvia Figàs-Segura, Àngela Benada, M'hamed Navarro-Herrero, Inmaculada Sampaio, Telma Maria G. Biosca, Elena Marco-Noales, Ester |
| author_browse | Barbé, Silvia Benada, M'hamed Figàs-Segura, Àngela G. Biosca, Elena Marco-Noales, Ester Navarro-Herrero, Inmaculada Sampaio, Telma Maria |
| author_facet | Barbé, Silvia Figàs-Segura, Àngela Benada, M'hamed Navarro-Herrero, Inmaculada Sampaio, Telma Maria G. Biosca, Elena Marco-Noales, Ester |
| author_sort | Barbé, Silvia |
| collection | ReDivia |
| description | Control of bacterial plant diseases is a major concern, as they affect economically important species and spread easily, such as the case of fire blight of rosaceous caused by Erwinia amylovora. In the search for alternatives to the use of agrochemicals and antibiotics, this work presents a screening of natural bacterial antagonists of this relevant and devastating phytopathogen. We recovered bacterial isolates from different plant tissues and geographical origins and then selected those with the strongest ability to reduce fire blight symptoms ex vivo and remarkable in vitro antagonistic activity against E. amylovora. None of them elicited a hypersensitivity reaction in tobacco leaves, most produced several hydrolytic enzymes and presented other biocontrol and/or plant growth-promoting activities, such as siderophore production and phosphate solubilization. These isolates, considered as biocontrol candidates, were identified by 16S rRNA sequencing as Pseudomonas rhizosphaerae, Curtobacterium flaccumfaciens, Enterobacter cancerogenus, Pseudomonas azotoformans, Rosenbergiella epipactidis and Serratia plymuthica. This is the first time that the last five bacterial species are reported to have biocontrol potential against E. amylovora. |
| format | acceptedVersion |
| id | ReDivia8053 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Society for Applied Microbiology and John Wiley & Sons Ltd. |
| publisherStr | Society for Applied Microbiology and John Wiley & Sons Ltd. |
| record_format | dspace |
| spelling | ReDivia80532025-04-25T14:48:39Z Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora Barbé, Silvia Figàs-Segura, Àngela Benada, M'hamed Navarro-Herrero, Inmaculada Sampaio, Telma Maria G. Biosca, Elena Marco-Noales, Ester Hydrolytic enzymes 16S rRNA H20 Plant diseases Biocontrol Loquats Pears Hypersensitivity Fire blight Control of bacterial plant diseases is a major concern, as they affect economically important species and spread easily, such as the case of fire blight of rosaceous caused by Erwinia amylovora. In the search for alternatives to the use of agrochemicals and antibiotics, this work presents a screening of natural bacterial antagonists of this relevant and devastating phytopathogen. We recovered bacterial isolates from different plant tissues and geographical origins and then selected those with the strongest ability to reduce fire blight symptoms ex vivo and remarkable in vitro antagonistic activity against E. amylovora. None of them elicited a hypersensitivity reaction in tobacco leaves, most produced several hydrolytic enzymes and presented other biocontrol and/or plant growth-promoting activities, such as siderophore production and phosphate solubilization. These isolates, considered as biocontrol candidates, were identified by 16S rRNA sequencing as Pseudomonas rhizosphaerae, Curtobacterium flaccumfaciens, Enterobacter cancerogenus, Pseudomonas azotoformans, Rosenbergiella epipactidis and Serratia plymuthica. This is the first time that the last five bacterial species are reported to have biocontrol potential against E. amylovora. 2022-04-28T07:59:15Z 2022-04-28T07:59:15Z 2022 acceptedVersion Barbé, S., Figàs-Segura, À., Benada, M., Navarro-Herrero, I., Sampaio, T. M., Biosca, E. G. et al. (2022). Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora. Environmental Microbiology Reports, 14(4), 559-569. PMID: 35403335 DOI: 10.1111/1758-2229.13064 http://hdl.handle.net/20.500.11939/8053 10.1111/1758-2229.13064 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13064 en info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2015–00087-C022 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2015–00087-C01 the Grants RTA2015-00087-C01 and RTA2015-00087-C02 funded by MCIN/AEI/10.13039/501100011033, the Spanish Instituto Nacional de Investigaci on y Tecnología Agraria y Alimentaria (INIA), and ‘ERDF A way of making Europe’, and for the Grant IVIA/52202D –Sostenible – funded by Instituto Valenciano de Investigaciones Agrarias, Generalitat Valenciana openAccess Society for Applied Microbiology and John Wiley & Sons Ltd. electronico |
| spellingShingle | Hydrolytic enzymes 16S rRNA H20 Plant diseases Biocontrol Loquats Pears Hypersensitivity Fire blight Barbé, Silvia Figàs-Segura, Àngela Benada, M'hamed Navarro-Herrero, Inmaculada Sampaio, Telma Maria G. Biosca, Elena Marco-Noales, Ester Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title | Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title_full | Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title_fullStr | Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title_full_unstemmed | Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title_short | Plant-associated microbiota as a source of antagonistic bacteria against the phytopathogen Erwinia amylovora |
| title_sort | plant associated microbiota as a source of antagonistic bacteria against the phytopathogen erwinia amylovora |
| topic | Hydrolytic enzymes 16S rRNA H20 Plant diseases Biocontrol Loquats Pears Hypersensitivity Fire blight |
| url | http://hdl.handle.net/20.500.11939/8053 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13064 |
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