Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota
Eukaryotes have established symbiotic relationship with microorganisms, which enables them to accomplish functions that they cannot perform alone. In the German cockroach, Blattella germanica, the obligate endosymbiont Blattabacterium coexists with a rich gut microbiota. The transmission of Blattaba...
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| Format: | article |
| Language: | Inglés |
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Oxford University Press
2022
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| Online Access: | http://hdl.handle.net/20.500.11939/8050 https://doi.org/10.1093/femsec/fiy002 https://academic.oup.com/femsec/article/94/2/fiy002/4794938?login=true |
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| author | Rosas, Tania García-Ferris, Carlos Domínguez-Santos, Rebeca Llop, Pablo Latorre, Amparo Moya, Andres |
| author_browse | Domínguez-Santos, Rebeca García-Ferris, Carlos Latorre, Amparo Llop, Pablo Moya, Andres Rosas, Tania |
| author_facet | Rosas, Tania García-Ferris, Carlos Domínguez-Santos, Rebeca Llop, Pablo Latorre, Amparo Moya, Andres |
| author_sort | Rosas, Tania |
| collection | ReDivia |
| description | Eukaryotes have established symbiotic relationship with microorganisms, which enables them to accomplish functions that they cannot perform alone. In the German cockroach, Blattella germanica, the obligate endosymbiont Blattabacterium coexists with a rich gut microbiota. The transmission of Blattabacterium is vertical, but little is known about how the gut microbiota colonizes newborn individuals. In this study, we treated B. germanica populations with rifampicin, a broad-spectrum antibiotic, during two generations and analyzed gut bacterial composition and the Blattabacterium load in control and rifampicin-treated populations. Rifampicin exerted a drastic effect on gut microbiota composition, which recovered in the second generation in the case where the antibiotic was not added to the diet. Furthermore, we observed that bacterial species present in the diet, and particularly in the feces, contribute significantly to establishing the gut microbiota. Finally, the Blattabacterium population remained unaffected by the antibiotic treatment of adults during the first generation but was strongly reduced in the second generation, suggesting that this intracellular symbiont is sensitive to rifampicin only during the infection of the mature oocytes, when it is in an extracellular stage. |
| format | article |
| id | ReDivia8050 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia80502025-04-25T14:48:43Z Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota Rosas, Tania García-Ferris, Carlos Domínguez-Santos, Rebeca Llop, Pablo Latorre, Amparo Moya, Andres Insect symbiosis Gut microbiota Endosymbiont Antibiotic treatment Microbiota transmission L70 Veterinary science and hygiene L73 Animal diseases L01 Animal husbandry Eukaryotes have established symbiotic relationship with microorganisms, which enables them to accomplish functions that they cannot perform alone. In the German cockroach, Blattella germanica, the obligate endosymbiont Blattabacterium coexists with a rich gut microbiota. The transmission of Blattabacterium is vertical, but little is known about how the gut microbiota colonizes newborn individuals. In this study, we treated B. germanica populations with rifampicin, a broad-spectrum antibiotic, during two generations and analyzed gut bacterial composition and the Blattabacterium load in control and rifampicin-treated populations. Rifampicin exerted a drastic effect on gut microbiota composition, which recovered in the second generation in the case where the antibiotic was not added to the diet. Furthermore, we observed that bacterial species present in the diet, and particularly in the feces, contribute significantly to establishing the gut microbiota. Finally, the Blattabacterium population remained unaffected by the antibiotic treatment of adults during the first generation but was strongly reduced in the second generation, suggesting that this intracellular symbiont is sensitive to rifampicin only during the infection of the mature oocytes, when it is in an extracellular stage. 2022-04-28T07:50:30Z 2022-04-28T07:50:30Z 2017 article publishedVersion Rosas, T., García-Ferris, C., Domínguez-Santos, R., Llop, P., Latorre, A. & Moya, A. (2018). Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota. FEMS Microbiology Ecology, 94(2), fiy002. 1574-6941 (Online ISSN) 0168-6496 (Print ISSN) http://hdl.handle.net/20.500.11939/8050 https://doi.org/10.1093/femsec/fiy002 https://academic.oup.com/femsec/article/94/2/fiy002/4794938?login=true en info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64322-C2-1-R info:eu-repo/grantAgreement/CEGVA/Prometeo 2014/065 This work was supported by grant BFU2015-64322-C2-1-R (cofinanced by FEDER funds and the Ministerio de Economía y Competitividad, Spain) to AL and PrometeoII/2014/065 (Conselleria d’Educacio, Generalitat Valenciana, Spain) to AM. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess Oxford University Press electronico |
| spellingShingle | Insect symbiosis Gut microbiota Endosymbiont Antibiotic treatment Microbiota transmission L70 Veterinary science and hygiene L73 Animal diseases L01 Animal husbandry Rosas, Tania García-Ferris, Carlos Domínguez-Santos, Rebeca Llop, Pablo Latorre, Amparo Moya, Andres Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title | Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title_full | Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title_fullStr | Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title_full_unstemmed | Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title_short | Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota |
| title_sort | rifampicin treatment of blattella germanica evidences a fecal transmission route of their gut microbiota |
| topic | Insect symbiosis Gut microbiota Endosymbiont Antibiotic treatment Microbiota transmission L70 Veterinary science and hygiene L73 Animal diseases L01 Animal husbandry |
| url | http://hdl.handle.net/20.500.11939/8050 https://doi.org/10.1093/femsec/fiy002 https://academic.oup.com/femsec/article/94/2/fiy002/4794938?login=true |
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