The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions
Xcc (Xanthomonas citri subsp. citri) causes citrus bacterial canker, a leaf, stem and fruit spotting disease that affects most commercial citrus species and cultivars. Copper compounds, widely used for management of this pathogen, have been reported as inducers of a VBNC (viable but non-culturable s...
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| Formato: | article |
| Lenguaje: | Inglés |
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David Publishing
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/8005 https://www.davidpublisher.com/index.php/Home/Article/index?id=9795.html |
| _version_ | 1855032711791509504 |
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| author | Golmohammadi, Morteza Cubero, Jaime López, María M. Llop, Pablo |
| author_browse | Cubero, Jaime Golmohammadi, Morteza Llop, Pablo López, María M. |
| author_facet | Golmohammadi, Morteza Cubero, Jaime López, María M. Llop, Pablo |
| author_sort | Golmohammadi, Morteza |
| collection | ReDivia |
| description | Xcc (Xanthomonas citri subsp. citri) causes citrus bacterial canker, a leaf, stem and fruit spotting disease that affects most commercial citrus species and cultivars. Copper compounds, widely used for management of this pathogen, have been reported as inducers of a VBNC (viable but non-culturable state) in plant pathogenic bacteria. VBNC may be considered as a state preceding bacterial death or as a survival mechanism under adverse conditions. Several experiments were performed to characterize the reversibility and persistence of the VBNC state in Xcc. VBNC was induced in low nutrient medium or with amendment of copper at concentrations used for field disease control. The VBNC condition was demonstrated to persist up to 150 days after copper treatment and was reversed after the addition of culture media without copper or amendment with citrus leaf extract. Xcc viability was evaluated by recovery of colonies on culture media, confirmed by membrane integrity, respiratory activity and by real-time RT-PCR targeting a sequence from the gumD gene. Besides, the colonies recovered were pathogenic on citrus leaves. These results confirm that the VBNC state in Xcc is inducible and reversible and therefore may occur in the phyllosphere when Xcc is under copper stress or starvation. |
| format | article |
| id | ReDivia8005 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | David Publishing |
| publisherStr | David Publishing |
| record_format | dspace |
| spelling | ReDivia80052025-04-25T14:48:41Z The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions Golmohammadi, Morteza Cubero, Jaime López, María M. Llop, Pablo VBNC (viable but non-culturable state) Copper stress Low nutrient availability Citrus bacterial canker H20 Plant diseases U30 Research methods Resuscitation Xanthomonas citri Citrus Xcc (Xanthomonas citri subsp. citri) causes citrus bacterial canker, a leaf, stem and fruit spotting disease that affects most commercial citrus species and cultivars. Copper compounds, widely used for management of this pathogen, have been reported as inducers of a VBNC (viable but non-culturable state) in plant pathogenic bacteria. VBNC may be considered as a state preceding bacterial death or as a survival mechanism under adverse conditions. Several experiments were performed to characterize the reversibility and persistence of the VBNC state in Xcc. VBNC was induced in low nutrient medium or with amendment of copper at concentrations used for field disease control. The VBNC condition was demonstrated to persist up to 150 days after copper treatment and was reversed after the addition of culture media without copper or amendment with citrus leaf extract. Xcc viability was evaluated by recovery of colonies on culture media, confirmed by membrane integrity, respiratory activity and by real-time RT-PCR targeting a sequence from the gumD gene. Besides, the colonies recovered were pathogenic on citrus leaves. These results confirm that the VBNC state in Xcc is inducible and reversible and therefore may occur in the phyllosphere when Xcc is under copper stress or starvation. 2022-03-30T10:31:01Z 2022-03-30T10:31:01Z 2013 article publishedVersion Golmohammadi, M., Cubero, J., Lopez, M. M. & Llop, P. (2013). The viable but non-culturable state in Xanthomonas citri subsp. citri is a reversible state induced by low nutrient availability and copper stress conditions. Journal of Life Sciences, 7(10), 1019-1029. 1934-7391 1934-7405 (on-line) http://hdl.handle.net/20.500.11939/8005 https://www.davidpublisher.com/index.php/Home/Article/index?id=9795.html en info:eu-repo/grantAgreement/MEC/Programa Nacional de Recursos y Tecnologías Agroalimentarias/RTA2006-00149-00-00//Desarrollo de métodos de detección y estudios de supervivencia de Xanthomonas causantes de la cancrosis de los cítricos info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/RTA2008-00048-C02/Estudios sobre la supervivencia de Xanthomonas axonopodis pv. citri y de las primeras etapas de la infección en la cancrosis de los cítricos This research was supported by INIA projects RTA2006-00149 and RTA2008-00048. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess David Publishing electronico |
| spellingShingle | VBNC (viable but non-culturable state) Copper stress Low nutrient availability Citrus bacterial canker H20 Plant diseases U30 Research methods Resuscitation Xanthomonas citri Citrus Golmohammadi, Morteza Cubero, Jaime López, María M. Llop, Pablo The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title | The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title_full | The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title_fullStr | The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title_full_unstemmed | The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title_short | The Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditions |
| title_sort | viable but non culturable state in xanthomonas citri subsp citri is a reversible state induced by low nutrient availability and copper stress conditions |
| topic | VBNC (viable but non-culturable state) Copper stress Low nutrient availability Citrus bacterial canker H20 Plant diseases U30 Research methods Resuscitation Xanthomonas citri Citrus |
| url | http://hdl.handle.net/20.500.11939/8005 https://www.davidpublisher.com/index.php/Home/Article/index?id=9795.html |
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