Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)

In 2017, Xylella fastidiosa, a quarantine plant-pathogenic bacterium in Europe, was detected in almond trees associated with leaf scorch symptoms in Alicante, a Mediterranean area in southeastern mainland Spain. The bacterium was detected by serological and molecular techniques, isolated in axenic c...

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Autores principales: Marco-Noales, Ester, Barbé, Silvia, Monterde, Adela, Navarro-Herrero, Inmaculada, Ferrer, Amparo, Dalmau, Vicente, Aure, Cristina M., Domingo-Calap, María L., Landa, Blanca B., Roselló, Montserrat
Formato: article
Lenguaje:Inglés
Publicado: American Phytopathological Society (APS) 2022
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Acceso en línea:http://hdl.handle.net/20.500.11939/7955
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-03-21-0625-SC
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author Marco-Noales, Ester
Barbé, Silvia
Monterde, Adela
Navarro-Herrero, Inmaculada
Ferrer, Amparo
Dalmau, Vicente
Aure, Cristina M.
Domingo-Calap, María L.
Landa, Blanca B.
Roselló, Montserrat
author_browse Aure, Cristina M.
Barbé, Silvia
Dalmau, Vicente
Domingo-Calap, María L.
Ferrer, Amparo
Landa, Blanca B.
Marco-Noales, Ester
Monterde, Adela
Navarro-Herrero, Inmaculada
Roselló, Montserrat
author_facet Marco-Noales, Ester
Barbé, Silvia
Monterde, Adela
Navarro-Herrero, Inmaculada
Ferrer, Amparo
Dalmau, Vicente
Aure, Cristina M.
Domingo-Calap, María L.
Landa, Blanca B.
Roselló, Montserrat
author_sort Marco-Noales, Ester
collection ReDivia
description In 2017, Xylella fastidiosa, a quarantine plant-pathogenic bacterium in Europe, was detected in almond trees associated with leaf scorch symptoms in Alicante, a Mediterranean area in southeastern mainland Spain. The bacterium was detected by serological and molecular techniques, isolated in axenic culture from diseased almond trees, and identified as X. fastidiosa subsp. multiplex sequence type (ST) 6. Inoculation experiments on almond plants in greenhouse trials with a characterized strain of X. fastidiosa subsp. multiplex ST6 isolated in the outbreak area have proved that it was able to multiply and systemically colonize inoculated plants. Disease symptoms characteristic of leaf scorch like those observed in the field were observed in the inoculated almond trees after 1 year. Furthermore, the pathogen was reisolated and identified by molecular tests. With the fulfillment of Koch’s postulates, we have demonstrated that X. fastidiosa is the causal agent of the almond leaf scorch disease in the Alicante outbreak
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia79552025-04-25T14:48:41Z Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula) Marco-Noales, Ester Barbé, Silvia Monterde, Adela Navarro-Herrero, Inmaculada Ferrer, Amparo Dalmau, Vicente Aure, Cristina M. Domingo-Calap, María L. Landa, Blanca B. Roselló, Montserrat Almond leaf scorch disease Molecular techniques H20 Plant diseases U30 Research methods Xylella fastidiosa Serological techniques Inoculation In 2017, Xylella fastidiosa, a quarantine plant-pathogenic bacterium in Europe, was detected in almond trees associated with leaf scorch symptoms in Alicante, a Mediterranean area in southeastern mainland Spain. The bacterium was detected by serological and molecular techniques, isolated in axenic culture from diseased almond trees, and identified as X. fastidiosa subsp. multiplex sequence type (ST) 6. Inoculation experiments on almond plants in greenhouse trials with a characterized strain of X. fastidiosa subsp. multiplex ST6 isolated in the outbreak area have proved that it was able to multiply and systemically colonize inoculated plants. Disease symptoms characteristic of leaf scorch like those observed in the field were observed in the inoculated almond trees after 1 year. Furthermore, the pathogen was reisolated and identified by molecular tests. With the fulfillment of Koch’s postulates, we have demonstrated that X. fastidiosa is the causal agent of the almond leaf scorch disease in the Alicante outbreak 2022-03-04T16:43:14Z 2022-03-04T16:43:14Z 2021 article publishedVersion Marco-Noales, E., Barbé, S., Monterde, A., Navarro-Herrero, I., Ferrer, A., Dalmau, V. et al. (2021). Evidence that Xylella fastidiosa is the causal agent of almond leaf scorch disease in Alicante, mainland Spain (Iberian Peninsula). Plant Disease, 105(11), 3349-3352. 0191-2917 1943-7692 (e-ISSN) http://hdl.handle.net/20.500.11939/7955 10.1094/PDIS-03-21-0625-SC https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-03-21-0625-SC en Info:eu-repo/grantAgreement/EC/H2020/635646 Info:eu-repo/grantAgreement/EC/H2020/727987 Info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2017-00004-C06-01 Info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2017-00004-C06-02 European Union’s Horizon 2020 Framework Programme Research and Innovation Program Grant/Award Number: 635646 Grant/Award Number: 727987. Spanish State Research Agency Programa Estatal de I+D Orientada a los Retos de la Sociedad Grant/Award Number: E-RTA2017-00004-C06-01 Grant/Award Number: E-RTA2017-00004-C06-02. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess American Phytopathological Society (APS) electronico
spellingShingle Almond leaf scorch disease
Molecular techniques
H20 Plant diseases
U30 Research methods
Xylella fastidiosa
Serological techniques
Inoculation
Marco-Noales, Ester
Barbé, Silvia
Monterde, Adela
Navarro-Herrero, Inmaculada
Ferrer, Amparo
Dalmau, Vicente
Aure, Cristina M.
Domingo-Calap, María L.
Landa, Blanca B.
Roselló, Montserrat
Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title_full Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title_fullStr Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title_full_unstemmed Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title_short Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula)
title_sort evidence that xylella fastidiosa is the causal agent of almond leaf scorch disease in alicante mainland spain iberian peninsula
topic Almond leaf scorch disease
Molecular techniques
H20 Plant diseases
U30 Research methods
Xylella fastidiosa
Serological techniques
Inoculation
url http://hdl.handle.net/20.500.11939/7955
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-03-21-0625-SC
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