Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm
Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was de...
| Main Authors: | , , , , |
|---|---|
| Format: | article |
| Language: | Inglés |
| Published: |
MDPI
2022
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7844 https://www.mdpi.com/2076-2615/10/12/2399 |
| _version_ | 1855032682527850496 |
|---|---|
| author | Mocé, Eva Lozano-Palazón, Salvador A. Martínez-Granell, María M. Mocé, Maria Lorena Gómez, Ernesto A. |
| author_browse | Gómez, Ernesto A. Lozano-Palazón, Salvador A. Martínez-Granell, María M. Mocé, Eva Mocé, Maria Lorena |
| author_facet | Mocé, Eva Lozano-Palazón, Salvador A. Martínez-Granell, María M. Mocé, Maria Lorena Gómez, Ernesto A. |
| author_sort | Mocé, Eva |
| collection | ReDivia |
| description | Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was developed, and the in vitro quality and in vivo fertility of these doses were compared with those chilled by means of a programmable water bath in the laboratory at a rate of −0.18 °C/min. Of the tested prototypes, the one that provided an optimal combination of the chilling rate (average of −0.09 °C/min) and time required to reach 4 °C (3 h 45 min) was selected for further testing. Immediately after chilling and 24 h later, the doses chilled in the prototype were determined to be of higher quality than the samples chilled in the programmable water bath. Finally, the kidding rate was similar between the doses chilled in the programmable water bath (61.7% ± 7.1%) and in the prototype (56.1% ± 5.9%). In conclusion, successful chilling of goat sperm doses during transport is possible, thereby accelerating the delivery of insemination doses. |
| format | article |
| id | ReDivia7844 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia78442025-04-25T14:48:36Z Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm Mocé, Eva Lozano-Palazón, Salvador A. Martínez-Granell, María M. Mocé, Maria Lorena Gómez, Ernesto A. Goat buck Liquid-stored semen Refrigerated semen In vitro quality In vitro fertility L53 Animal physiology - Reproduction L70 Veterinary science and hygiene Cooling Chilling Semen In vitro Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was developed, and the in vitro quality and in vivo fertility of these doses were compared with those chilled by means of a programmable water bath in the laboratory at a rate of −0.18 °C/min. Of the tested prototypes, the one that provided an optimal combination of the chilling rate (average of −0.09 °C/min) and time required to reach 4 °C (3 h 45 min) was selected for further testing. Immediately after chilling and 24 h later, the doses chilled in the prototype were determined to be of higher quality than the samples chilled in the programmable water bath. Finally, the kidding rate was similar between the doses chilled in the programmable water bath (61.7% ± 7.1%) and in the prototype (56.1% ± 5.9%). In conclusion, successful chilling of goat sperm doses during transport is possible, thereby accelerating the delivery of insemination doses. 2022-01-13T08:31:21Z 2022-01-13T08:31:21Z 2020 article publishedVersion Mocé, E., Lozano-Palazón, S. A., Martínez-Granell, M. M., Mocé, M. L. & Gómez, E. A. (2020). Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm. Animals, 10(12), 2399. 2076-2615 http://hdl.handle.net/20.500.11939/7844 10.3390/ani10122399 https://www.mdpi.com/2076-2615/10/12/2399 en info:eu-repo/grantAgreement/MINECO//RTA2017-00049-C02-01/Uso de espectroscopia FT-MIR en el programa de mejora genética de la raza MurcianoGranadina: evaluación de calidad seminal y posibilidad de nuevos caracteres en las hembras info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51906 This research was funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and cofinanced by FEDER funds (grant number RTA2017-00049-C02-01), GVA-IVIA (grant number 51906) and AMURVAL (grant number 71714). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Goat buck Liquid-stored semen Refrigerated semen In vitro quality In vitro fertility L53 Animal physiology - Reproduction L70 Veterinary science and hygiene Cooling Chilling Semen In vitro Mocé, Eva Lozano-Palazón, Salvador A. Martínez-Granell, María M. Mocé, Maria Lorena Gómez, Ernesto A. Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title | Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title_full | Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title_fullStr | Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title_full_unstemmed | Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title_short | Effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| title_sort | effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| topic | Goat buck Liquid-stored semen Refrigerated semen In vitro quality In vitro fertility L53 Animal physiology - Reproduction L70 Veterinary science and hygiene Cooling Chilling Semen In vitro |
| url | http://hdl.handle.net/20.500.11939/7844 https://www.mdpi.com/2076-2615/10/12/2399 |
| work_keys_str_mv | AT moceeva effectoftherefrigerationsystemoninvitroqualityandinvivofertilityofgoatbucksperm AT lozanopalazonsalvadora effectoftherefrigerationsystemoninvitroqualityandinvivofertilityofgoatbucksperm AT martinezgranellmariam effectoftherefrigerationsystemoninvitroqualityandinvivofertilityofgoatbucksperm AT mocemarialorena effectoftherefrigerationsystemoninvitroqualityandinvivofertilityofgoatbucksperm AT gomezernestoa effectoftherefrigerationsystemoninvitroqualityandinvivofertilityofgoatbucksperm |