Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to s...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7842 https://www.mdpi.com/2311-7524/7/11/447 |
| _version_ | 1855032682188111872 |
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| author | Primo-Capella, Amparo Martínez-Cuenca, Mary-Rus Forner-Giner, María A. |
| author_browse | Forner-Giner, María A. Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo |
| author_facet | Primo-Capella, Amparo Martínez-Cuenca, Mary-Rus Forner-Giner, María A. |
| author_sort | Primo-Capella, Amparo |
| collection | ReDivia |
| description | Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the Poncirus trifoliata and Citrus macrophylla rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1 and COR413 IM1 was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes CAMTA3, CAMTA5, CBF1 and COR413 IM1 was seen in the tolerant genotype P. trifoliata. We quantified the gene expression of proline biosynthesis P5CS1, dOAT and the proline transporters PROT1 and PROT2; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time. |
| format | article |
| id | ReDivia7842 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia78422025-04-25T14:48:36Z Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks Primo-Capella, Amparo Martínez-Cuenca, Mary-Rus Forner-Giner, María A. Breeding programs F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry H50 Miscellaneous plant disorders U30 Research methods Citrus Rootstocks Cold stress Abiotic stress Gene expression Climate change Cold tolerance Poncirus trifoliata Citrus macrophylla Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the Poncirus trifoliata and Citrus macrophylla rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1 and COR413 IM1 was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes CAMTA3, CAMTA5, CBF1 and COR413 IM1 was seen in the tolerant genotype P. trifoliata. We quantified the gene expression of proline biosynthesis P5CS1, dOAT and the proline transporters PROT1 and PROT2; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time. 2022-01-03T19:07:34Z 2022-01-03T19:07:34Z 2021 article publishedVersion Primo-Capella, A., Martinez-Cuenca, M. R. & Forner-Giner, M. A. (2021). Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks. Horticulturae, 7(11), 447. 2311-7524 http://hdl.handle.net/20.500.11939/7842 10.3390/horticulturae7110447 https://www.mdpi.com/2311-7524/7/11/447 en info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018–098379-R-I00//OBTENCION, SELECCION, CARACTERIZACION Y EVALUACION DE NUEVOS PATRONES DE CITRICOS info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51913 This study was cofunded by the Ministerio de Ciencia e Innovación (RTI2018-098379-RI00) and the European Union through the European Regional Development Fund (ERDF) of the Generalitat Valenciana 2014-2020 (IVIA-51913). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico |
| spellingShingle | Breeding programs F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry H50 Miscellaneous plant disorders U30 Research methods Citrus Rootstocks Cold stress Abiotic stress Gene expression Climate change Cold tolerance Poncirus trifoliata Citrus macrophylla Primo-Capella, Amparo Martínez-Cuenca, Mary-Rus Forner-Giner, María A. Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title | Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title_full | Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title_fullStr | Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title_full_unstemmed | Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title_short | Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks |
| title_sort | gene expression under short term low temperatures preliminary screening method to obtain tolerant citrus rootstocks |
| topic | Breeding programs F30 Plant genetics and breeding F40 Plant ecology F60 Plant physiology and biochemistry H50 Miscellaneous plant disorders U30 Research methods Citrus Rootstocks Cold stress Abiotic stress Gene expression Climate change Cold tolerance Poncirus trifoliata Citrus macrophylla |
| url | http://hdl.handle.net/20.500.11939/7842 https://www.mdpi.com/2311-7524/7/11/447 |
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