Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations
Two field sources (PM 26-X and Cl 13-24) were subjected to biological and biochemical analysis for citrus viroids. PM 26-X was recovered from Frost Marsh grapefruit grafted on alemow showing chlorosis, stunting and gum impregnation of the bark, and Cl 13-24 was recovered from a symptomless Clementin...
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7837 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/258-263%20IOCV15.pdf |
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| author | Velázquez, Karelia Soto, Marianella Perez, Rosa Rodríguez, D. Durán-Vila, Núria |
| author2 | Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Perez, Rosa Rodríguez, D. Soto, Marianella Velázquez, Karelia |
| author_facet | Durán-Vila, Núria Velázquez, Karelia Soto, Marianella Perez, Rosa Rodríguez, D. Durán-Vila, Núria |
| author_sort | Velázquez, Karelia |
| collection | ReDivia |
| description | Two field sources (PM 26-X and Cl 13-24) were subjected to biological and biochemical analysis for citrus viroids. PM 26-X was recovered from Frost Marsh grapefruit grafted on alemow showing chlorosis, stunting and gum impregnation of the bark, and Cl 13-24 was recovered from a symptomless Clementine tree grafted on Troyer citrange. Biological indexing on Etrog citron, Parson’s Special mandarin and clemeline 11-20 showed symptoms characteristic of citrus viroids. Viroid infection was demonstrated by sPAGE and molecular hybridization analysis of nucleic acid preparations from the inoculated citrons. The nucleic acid extracts were subjected to retrotranscription, PCR amplification, cloning, SSCP analysis and sequencing. In all instances a “master sequence” was identified as the most frequent variant and was compared to other reported sequences of the same viroids. |
| format | Objeto de conferencia |
| id | ReDivia7837 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia78372025-04-25T14:54:07Z Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations Velázquez, Karelia Soto, Marianella Perez, Rosa Rodríguez, D. Durán-Vila, Núria Durán-Vila, Núria Citrus viroids Cachexia H20 Plant diseases U30 Research methods Symptoms Chlorosis Citrus Virus indexing Viroids Two field sources (PM 26-X and Cl 13-24) were subjected to biological and biochemical analysis for citrus viroids. PM 26-X was recovered from Frost Marsh grapefruit grafted on alemow showing chlorosis, stunting and gum impregnation of the bark, and Cl 13-24 was recovered from a symptomless Clementine tree grafted on Troyer citrange. Biological indexing on Etrog citron, Parson’s Special mandarin and clemeline 11-20 showed symptoms characteristic of citrus viroids. Viroid infection was demonstrated by sPAGE and molecular hybridization analysis of nucleic acid preparations from the inoculated citrons. The nucleic acid extracts were subjected to retrotranscription, PCR amplification, cloning, SSCP analysis and sequencing. In all instances a “master sequence” was identified as the most frequent variant and was compared to other reported sequences of the same viroids. 2021-12-28T12:47:33Z 2021-12-28T12:47:33Z 2002 conferenceObject Velazquez, K., Soto, M., Perez, R., Perez, J. M., Rodriguez, D. & Duran-Vila, N. (2002). Biological and molecular characterization of two isolates of citrus viroids recovered from Cuban plantations. Proceedings of the Fifteenth Conference of the International Organization of Citrus Virologists, 258-263. 2313-5123 http://hdl.handle.net/20.500.11939/7837 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/258-263%20IOCV15.pdf en 2001-11-11 Fifteenth Conference of the International Organization of Citrus Virologists Paphos, Cyprus Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Citrus viroids Cachexia H20 Plant diseases U30 Research methods Symptoms Chlorosis Citrus Virus indexing Viroids Velázquez, Karelia Soto, Marianella Perez, Rosa Rodríguez, D. Durán-Vila, Núria Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title | Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title_full | Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title_fullStr | Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title_full_unstemmed | Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title_short | Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations |
| title_sort | biological and molecular characterization of two isolates of citrus viroids recovered from cuban plantations |
| topic | Citrus viroids Cachexia H20 Plant diseases U30 Research methods Symptoms Chlorosis Citrus Virus indexing Viroids |
| url | http://hdl.handle.net/20.500.11939/7837 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/258-263%20IOCV15.pdf |
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