Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus
To develop RNA-mediated resistance (RMR) against Citrus tristeza virus (CTV), Mexican lime plants were transformed with two modified versions of CTV p25 gene: I) the complete p25 ORF derived from the severe strain T305, mutated to make its mRNA untranslatable, and II) an untranslatable fragment of t...
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7816 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/341-344%20IOCV15.pdf |
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| author | Domínguez, A. Fagoaga, Carmen Navarro, Luis Moreno, Pedro Pena, Leandro |
| author2 | Durán-Vila, Núria |
| author_browse | Domínguez, A. Durán-Vila, Núria Fagoaga, Carmen Moreno, Pedro Navarro, Luis Pena, Leandro |
| author_facet | Durán-Vila, Núria Domínguez, A. Fagoaga, Carmen Navarro, Luis Moreno, Pedro Pena, Leandro |
| author_sort | Domínguez, A. |
| collection | ReDivia |
| description | To develop RNA-mediated resistance (RMR) against Citrus tristeza virus (CTV), Mexican lime plants were transformed with two modified versions of CTV p25 gene: I) the complete p25 ORF derived from the severe strain T305, mutated to make its mRNA untranslatable, and II) an untranslatable fragment of the p25 gene fused to the green fluorescent protein (gfp) gene. Forty-eight independent transgenic lines were obtained in total, and Southern blot analyses revealed that all incorporated the corresponding p25 sequence. Some of them showed post-transcriptional gene silencing (PTGS) of the p25 transgene, which has been associated to RMR in other transgenic plant-virus interactions. After graft-inoculation with the homologous CTV strain, transgenic plants developed symptoms and virus accumulated at similar rates than in non-transgenic control plants. Factors potentially involved in this protection failure are discussed. |
| format | conferenceObject |
| id | ReDivia7816 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia78162025-04-25T14:52:54Z Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus Domínguez, A. Fagoaga, Carmen Navarro, Luis Moreno, Pedro Pena, Leandro Durán-Vila, Núria Graft-inoculation p25 gene Cross protection H20 Plant diseases U30 Research methods Citrus tristeza virus Inoculation Plant diseases Gene manipulation Resistance to disease Transgenic plants To develop RNA-mediated resistance (RMR) against Citrus tristeza virus (CTV), Mexican lime plants were transformed with two modified versions of CTV p25 gene: I) the complete p25 ORF derived from the severe strain T305, mutated to make its mRNA untranslatable, and II) an untranslatable fragment of the p25 gene fused to the green fluorescent protein (gfp) gene. Forty-eight independent transgenic lines were obtained in total, and Southern blot analyses revealed that all incorporated the corresponding p25 sequence. Some of them showed post-transcriptional gene silencing (PTGS) of the p25 transgene, which has been associated to RMR in other transgenic plant-virus interactions. After graft-inoculation with the homologous CTV strain, transgenic plants developed symptoms and virus accumulated at similar rates than in non-transgenic control plants. Factors potentially involved in this protection failure are discussed. 2021-12-11T11:58:44Z 2021-12-11T11:58:44Z 2002 conferenceObject Dominguez, A., Fagoaga, C., Navarro, L., Moreno, P. & Peña, L. (2002). Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus. Proceedings of the Fifteenth Conference of the International Organization of Citrus Virologists, 341-344 2313-5123 http://hdl.handle.net/20.500.11939/7816 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/341-344%20IOCV15.pdf en 2001-11-11 Fifteenth Conference of the International Organization of Citrus Virologists Paphos, Cyprus Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Graft-inoculation p25 gene Cross protection H20 Plant diseases U30 Research methods Citrus tristeza virus Inoculation Plant diseases Gene manipulation Resistance to disease Transgenic plants Domínguez, A. Fagoaga, Carmen Navarro, Luis Moreno, Pedro Pena, Leandro Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title | Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title_full | Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title_fullStr | Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title_full_unstemmed | Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title_short | Constitutive Expression of Untranslatable Versions of the p25 Coat Protein Gene of Citrus tristeza virus (CTV) in Transgenic Mexican Lime Plants Does Not Confer Resistance to the Virus |
| title_sort | constitutive expression of untranslatable versions of the p25 coat protein gene of citrus tristeza virus ctv in transgenic mexican lime plants does not confer resistance to the virus |
| topic | Graft-inoculation p25 gene Cross protection H20 Plant diseases U30 Research methods Citrus tristeza virus Inoculation Plant diseases Gene manipulation Resistance to disease Transgenic plants |
| url | http://hdl.handle.net/20.500.11939/7816 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/341-344%20IOCV15.pdf |
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