Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd)
Natural infections of Citrus exocortis viroid (CEVd) have been found in both citrus and non-citrus hosts. Field isolates of CEVd are complex populations of sequence variants (haplotypes) that fit a quasispecies model. We have previously shown that a CEVd isolate initially recovered from citrus and m...
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| Format: | Objeto de conferencia |
| Language: | Inglés |
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IOCV
2021
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| Online Access: | http://hdl.handle.net/20.500.11939/7814 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/291-300%20IOCV%2016.pdf |
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| author | Bernad, Lucia Gandía, Mónica Durán-Vila, Núria |
| author2 | Hilf, M. E. |
| author_browse | Bernad, Lucia Durán-Vila, Núria Gandía, Mónica Hilf, M. E. |
| author_facet | Hilf, M. E. Bernad, Lucia Gandía, Mónica Durán-Vila, Núria |
| author_sort | Bernad, Lucia |
| collection | ReDivia |
| description | Natural infections of Citrus exocortis viroid (CEVd) have been found in both citrus and non-citrus hosts. Field isolates of CEVd are complex populations of sequence variants (haplotypes) that fit a quasispecies model. We have previously shown that a CEVd isolate initially recovered from citrus and maintained in Etrog citron contained two predominant haplotypes with low nucleotide diversity. This CEVd infected citron was used as the inoculum source for viroid transmission to trifoliate orange and sour orange seedlings that have been growing in the field since their inoculation in 1993. CEVd recovered from trifoliate orange showing the characteristic symptoms of stunting, bark scaling and stem blotching was also characterized as a population of variants with low nucleotide diversity and containing a clearly predominant haplotype distinct from those identified in the inoculum source. CEVd recovered from sour orange, which remained symptomless, was characterized as a population with a higher nucleotide diversity in which no dominant haplotype could be identified. The tolerant viroid-host combination appeared to act as permissive host and maintained a greater CEVd variability than sensitive hosts. Similarly, a CEVd isolate recovered from a symptomless broad bean plant was characterized as a diverse population that lacked a predominant haplotype. When this CEVd source was mechanically transmitted to tomato and from tomato back to broad bean, the plants displayed stunting symptoms, and the CEVd population exhibited a predominant haplotype and low nucleotide diversity. |
| format | Objeto de conferencia |
| id | ReDivia7814 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia78142025-04-25T14:52:47Z Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) Bernad, Lucia Gandía, Mónica Durán-Vila, Núria Hilf, M. E. Quasispecies Citrus exocortis viroid Haplotypes H20 Plant diseases U30 Research methods Variants Natural infections of Citrus exocortis viroid (CEVd) have been found in both citrus and non-citrus hosts. Field isolates of CEVd are complex populations of sequence variants (haplotypes) that fit a quasispecies model. We have previously shown that a CEVd isolate initially recovered from citrus and maintained in Etrog citron contained two predominant haplotypes with low nucleotide diversity. This CEVd infected citron was used as the inoculum source for viroid transmission to trifoliate orange and sour orange seedlings that have been growing in the field since their inoculation in 1993. CEVd recovered from trifoliate orange showing the characteristic symptoms of stunting, bark scaling and stem blotching was also characterized as a population of variants with low nucleotide diversity and containing a clearly predominant haplotype distinct from those identified in the inoculum source. CEVd recovered from sour orange, which remained symptomless, was characterized as a population with a higher nucleotide diversity in which no dominant haplotype could be identified. The tolerant viroid-host combination appeared to act as permissive host and maintained a greater CEVd variability than sensitive hosts. Similarly, a CEVd isolate recovered from a symptomless broad bean plant was characterized as a diverse population that lacked a predominant haplotype. When this CEVd source was mechanically transmitted to tomato and from tomato back to broad bean, the plants displayed stunting symptoms, and the CEVd population exhibited a predominant haplotype and low nucleotide diversity. 2021-12-11T11:58:25Z 2021-12-11T11:58:25Z 2005 conferenceObject Bernad, L. Gandia, L. & Durán-Vila, N. (2005). Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd). Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 291-300. 2313-5123 http://hdl.handle.net/20.500.11939/7814 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/291-300%20IOCV%2016.pdf en 2004-11-07 Sixteenth Conference of the International Organization of Citrus Virologists Monterrey, Mexico This work was partially supported by RTA01-119 from the Ministerio de Ciencia y Tecnología. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Quasispecies Citrus exocortis viroid Haplotypes H20 Plant diseases U30 Research methods Variants Bernad, Lucia Gandía, Mónica Durán-Vila, Núria Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title | Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title_full | Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title_fullStr | Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title_full_unstemmed | Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title_short | Host Effect on the Genetic Variability of Citrus exocortis viroid (CEVd) |
| title_sort | host effect on the genetic variability of citrus exocortis viroid cevd |
| topic | Quasispecies Citrus exocortis viroid Haplotypes H20 Plant diseases U30 Research methods Variants |
| url | http://hdl.handle.net/20.500.11939/7814 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/291-300%20IOCV%2016.pdf |
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