Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus

The effect of citrus tristeza virus (CTV) infection on bark proteins of several citrus rootstocks was studied by polyacrylamide gel electrophoresis (SDSPAGE). A protein band (P6) from bark of sour orange grafted with sweet orange, grapefruit or clementine scions had lower intensity in CTV-infected t...

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Autores principales: Moreno, Pedro, Ortiz Marcide, Jesús M., Guerri, José
Otros Autores: Garnsey, Stephen M.
Formato: Objeto de conferencia
Lenguaje:Inglés
Publicado: IOCV 2021
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/7789
https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th081_085.pdf
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author Moreno, Pedro
Ortiz Marcide, Jesús M.
Guerri, José
author2 Garnsey, Stephen M.
author_browse Garnsey, Stephen M.
Guerri, José
Moreno, Pedro
Ortiz Marcide, Jesús M.
author_facet Garnsey, Stephen M.
Moreno, Pedro
Ortiz Marcide, Jesús M.
Guerri, José
author_sort Moreno, Pedro
collection ReDivia
description The effect of citrus tristeza virus (CTV) infection on bark proteins of several citrus rootstocks was studied by polyacrylamide gel electrophoresis (SDSPAGE). A protein band (P6) from bark of sour orange grafted with sweet orange, grapefruit or clementine scions had lower intensity in CTV-infected than in CTV-free trees. Decrease of P6 intensity was more conspicuous in trees infected with a severe CTV isolate than in those infected with a mild isolate. No difference was observed between bark proteins of CTV infected and CTV-free sour orange sprouts. Protein profiles of healthy and tristeza-infected Troyer citrange or Cleopatra mandarin, grafted with navel orange, were also indistinguishable. Finally, protein patterns of sour orange grafted with navel orange, severely declined from Phytophthora sp. or psorosis, were similar to those of non-declined controls. Thus, decreased intensity of P6 seems to be specifically associated with tristeza in citrus trees grafted on sour orange.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia77892025-04-25T14:54:02Z Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus Moreno, Pedro Ortiz Marcide, Jesús M. Guerri, José Garnsey, Stephen M. Slab gel electrohoresis Tristeza decline H20 Plant diseases U30 Research methods Plant protein Sour orange Citrus tristeza virus The effect of citrus tristeza virus (CTV) infection on bark proteins of several citrus rootstocks was studied by polyacrylamide gel electrophoresis (SDSPAGE). A protein band (P6) from bark of sour orange grafted with sweet orange, grapefruit or clementine scions had lower intensity in CTV-infected than in CTV-free trees. Decrease of P6 intensity was more conspicuous in trees infected with a severe CTV isolate than in those infected with a mild isolate. No difference was observed between bark proteins of CTV infected and CTV-free sour orange sprouts. Protein profiles of healthy and tristeza-infected Troyer citrange or Cleopatra mandarin, grafted with navel orange, were also indistinguishable. Finally, protein patterns of sour orange grafted with navel orange, severely declined from Phytophthora sp. or psorosis, were similar to those of non-declined controls. Thus, decreased intensity of P6 seems to be specifically associated with tristeza in citrus trees grafted on sour orange. 2021-11-30T09:15:50Z 2021-11-30T09:15:50Z 1984 conferenceObject Moreno, P., Ortiz-Marcide, J. & Guerri, J. (1984). Changes in the bark proteins of sour orange rootstock induced by citrus tristeza virus. Proceedings of the Fifteenth Conference of the International Organization of Citrus Virologists, 81-85. 2313-5123 http://hdl.handle.net/20.500.11939/7789 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th081_085.pdf en 1983-05-09 Ninth Conference of the International Organization of Citrus Virologists Argentina Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico
spellingShingle Slab gel electrohoresis
Tristeza decline
H20 Plant diseases
U30 Research methods
Plant protein
Sour orange
Citrus tristeza virus
Moreno, Pedro
Ortiz Marcide, Jesús M.
Guerri, José
Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title_full Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title_fullStr Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title_full_unstemmed Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title_short Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus
title_sort changes in the bark proteins of sour orange rootstock induced by citrus tristeza virus
topic Slab gel electrohoresis
Tristeza decline
H20 Plant diseases
U30 Research methods
Plant protein
Sour orange
Citrus tristeza virus
url http://hdl.handle.net/20.500.11939/7789
https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th081_085.pdf
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