Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera
Plants of 32 species of Citrus and related genera grafted on rough lemon rootstock were inoculated with an artificial mixture of citrus viroids containing Citrus exocortis viroid , Citrus viroid I, Citrus viroid II, Citrus viroid III and Citrus viroid IV . Infection and viroid titers were determined...
| Main Authors: | , , , |
|---|---|
| Format: | conferenceObject |
| Language: | Inglés |
| Published: |
IOCV
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7788 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV15.pdf |
| _version_ | 1855032670499635200 |
|---|---|
| author | Barbosa, C. J. Pina, José A. Navarro, Luis Durán-Vila, Núria |
| author_browse | Barbosa, C. J. Durán-Vila, Núria Navarro, Luis Pina, José A. |
| author_facet | Barbosa, C. J. Pina, José A. Navarro, Luis Durán-Vila, Núria |
| author_sort | Barbosa, C. J. |
| collection | ReDivia |
| description | Plants of 32 species of Citrus and related genera grafted on rough lemon rootstock were inoculated with an artificial mixture of citrus viroids containing Citrus exocortis viroid , Citrus viroid I, Citrus viroid II, Citrus viroid III and Citrus viroid IV . Infection and viroid titers were determined by sPAGE and molecular hybridization analysis. Plants in which viroid infection could not be detected were further indexed by inoculation on Etrog citron 861 S1. Under the conditions of this assay most of the species were symptomless carriers. Only Citrus excelsa, C. ichangensis, C. karna, C. latifolia, C. meyeri and C. pyriformis developed symptoms as a result of viroid infection. Comparative analysis of nucleic acid extracts from bark versus leaf blade tissues indicated that in 10 species, viroid that were readily detectable from bark were undetectable from leaf blade tissues by sPAGE. |
| format | conferenceObject |
| id | ReDivia7788 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77882025-04-25T14:54:01Z Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera Barbosa, C. J. Pina, José A. Navarro, Luis Durán-Vila, Núria Rough lemon rootstock IVIA's germplasm bank F30 Plant genetics and breeding H20 Plant diseases U30 Research methods Citrus Viroids Symptoms Plants of 32 species of Citrus and related genera grafted on rough lemon rootstock were inoculated with an artificial mixture of citrus viroids containing Citrus exocortis viroid , Citrus viroid I, Citrus viroid II, Citrus viroid III and Citrus viroid IV . Infection and viroid titers were determined by sPAGE and molecular hybridization analysis. Plants in which viroid infection could not be detected were further indexed by inoculation on Etrog citron 861 S1. Under the conditions of this assay most of the species were symptomless carriers. Only Citrus excelsa, C. ichangensis, C. karna, C. latifolia, C. meyeri and C. pyriformis developed symptoms as a result of viroid infection. Comparative analysis of nucleic acid extracts from bark versus leaf blade tissues indicated that in 10 species, viroid that were readily detectable from bark were undetectable from leaf blade tissues by sPAGE. 2021-11-30T09:15:40Z 2021-11-30T09:15:40Z 2002 conferenceObject Barbosa, C., Pina, J. A., Navarro, L. & Duran-Vila, N. (2002). Replication/accumulation and symptom expression of citrus viroids on some species of citrus and related genera. Proceedings of the Fifteenth Conference of the International Organization of Citrus Virologists, 264-271. 2313-5123 http://hdl.handle.net/20.500.11939/7788 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV15.pdf en 2001-11-11 Fifteenth Conference of the International Organization of Citrus Virologists Paphos, Cyprus This work was supported by INIA grant RTA01-119 and a fellowship provided by EMBRAPA/CAPES Foundation to C. J. Barbosa. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Rough lemon rootstock IVIA's germplasm bank F30 Plant genetics and breeding H20 Plant diseases U30 Research methods Citrus Viroids Symptoms Barbosa, C. J. Pina, José A. Navarro, Luis Durán-Vila, Núria Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title | Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title_full | Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title_fullStr | Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title_full_unstemmed | Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title_short | Replication/Accumulation and Symptom Expression of Citrus Viroids on Some Species of Citrus and Related Genera |
| title_sort | replication accumulation and symptom expression of citrus viroids on some species of citrus and related genera |
| topic | Rough lemon rootstock IVIA's germplasm bank F30 Plant genetics and breeding H20 Plant diseases U30 Research methods Citrus Viroids Symptoms |
| url | http://hdl.handle.net/20.500.11939/7788 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/264-271%20IOCV15.pdf |
| work_keys_str_mv | AT barbosacj replicationaccumulationandsymptomexpressionofcitrusviroidsonsomespeciesofcitrusandrelatedgenera AT pinajosea replicationaccumulationandsymptomexpressionofcitrusviroidsonsomespeciesofcitrusandrelatedgenera AT navarroluis replicationaccumulationandsymptomexpressionofcitrusviroidsonsomespeciesofcitrusandrelatedgenera AT duranvilanuria replicationaccumulationandsymptomexpressionofcitrusviroidsonsomespeciesofcitrusandrelatedgenera |