Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks
Citrus leaf blotch virus (CLBV) was first detected in a Nagami kumquat, clone SRA-153 from Corsica (France), showing bud union crease when propagated on Troyer citrange. Citranges are important rootstocks in Spain, presently being used for about 50% of all commercial citrus trees. Therefore, dispers...
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7787 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/205-212%20IOCV15.pdf |
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| author | Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José |
| author2 | Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Galipienso, Luis Guerri, José Moreno, Pedro Navarro, Luis Vives, María C. |
| author_facet | Durán-Vila, Núria Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Vives, María C. |
| collection | ReDivia |
| description | Citrus leaf blotch virus (CLBV) was first detected in a Nagami kumquat, clone SRA-153 from Corsica (France), showing bud union crease when propagated on Troyer citrange. Citranges are important rootstocks in Spain, presently being used for about 50% of all commercial citrus trees. Therefore, dispersal of a graft-transmissible pathogen causing bud union crease on this rootstock could potentially cause important economic losses. CLBV has filamentous particles about 900×14 nm in size, with a single-stranded, positive sense, genomic RNA (gRNA) of 8,747nt, and a coat protein about 41 kDa. The gRNA contains three open reading frames (ORFs) and two untranslated regions of 73 and 541 nt at the 5’ and 3’ termini, respectively. Biological and molecular properties of CLBV support its inclusion in a new virus genus. The virus can be detected by graft-inoculation onto Dweet tangor seedlings, in which it induces chlorotic blotching in young leaves, but transmission is sometimes erratic and at least six indicator plants should be used in each test. We developed a quick detection procedure using RT-PCR with two sets of primers derived from sequences in ORF1 (a region containing motifs characteristic of an RNA polymerase) and ORF3 (the coat protein gene). Results with both sets of primers were similar. CLBV was readily detected in young leaves of infected Nagami kumquat or in Nules Clementine, Owari
Satsuma, Eureka lemon, Marsh grapefruit or Newhall navel orange inoculated with kumquat SRA-153, but not in Pineapple sweet orange, a host that yielded more than 80% false negatives. Detection in field trees was less consistent, as the virus generally has low titer and is unevenly distributed. By this procedure CLBV was detected in two mandarins from Japan, a kumquat from New South Wales (Australia), and in various sweet orange trees showing bud union crease on citrange or citrumelo, from commercial citrus orchards in Valencia (Spain) and Florida (USA), but not in other trees in the same orchards showing similar symptoms. Our results indicate that CLBV is present in citrus varieties other than kumquat in several geographic areas. Failure to detect CLBV in some trees with bud union crease could be due to low titer or uneven distribution of the virus within the plant. Alternatively, a different agent could be involved. |
| format | conferenceObject |
| id | ReDivia7787 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77872025-04-25T14:53:52Z Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José Durán-Vila, Núria Citrus leaf blotch virus Plant viruses PCR Rootstocks Citrus leaf blotch virus (CLBV) was first detected in a Nagami kumquat, clone SRA-153 from Corsica (France), showing bud union crease when propagated on Troyer citrange. Citranges are important rootstocks in Spain, presently being used for about 50% of all commercial citrus trees. Therefore, dispersal of a graft-transmissible pathogen causing bud union crease on this rootstock could potentially cause important economic losses. CLBV has filamentous particles about 900×14 nm in size, with a single-stranded, positive sense, genomic RNA (gRNA) of 8,747nt, and a coat protein about 41 kDa. The gRNA contains three open reading frames (ORFs) and two untranslated regions of 73 and 541 nt at the 5’ and 3’ termini, respectively. Biological and molecular properties of CLBV support its inclusion in a new virus genus. The virus can be detected by graft-inoculation onto Dweet tangor seedlings, in which it induces chlorotic blotching in young leaves, but transmission is sometimes erratic and at least six indicator plants should be used in each test. We developed a quick detection procedure using RT-PCR with two sets of primers derived from sequences in ORF1 (a region containing motifs characteristic of an RNA polymerase) and ORF3 (the coat protein gene). Results with both sets of primers were similar. CLBV was readily detected in young leaves of infected Nagami kumquat or in Nules Clementine, Owari Satsuma, Eureka lemon, Marsh grapefruit or Newhall navel orange inoculated with kumquat SRA-153, but not in Pineapple sweet orange, a host that yielded more than 80% false negatives. Detection in field trees was less consistent, as the virus generally has low titer and is unevenly distributed. By this procedure CLBV was detected in two mandarins from Japan, a kumquat from New South Wales (Australia), and in various sweet orange trees showing bud union crease on citrange or citrumelo, from commercial citrus orchards in Valencia (Spain) and Florida (USA), but not in other trees in the same orchards showing similar symptoms. Our results indicate that CLBV is present in citrus varieties other than kumquat in several geographic areas. Failure to detect CLBV in some trees with bud union crease could be due to low titer or uneven distribution of the virus within the plant. Alternatively, a different agent could be involved. 2021-11-25T17:15:03Z 2021-11-25T17:15:03Z 2002 conferenceObject Vives, M. C., Galipienso, L., Navarro, L. Moreno, P. & Guerri, J. (2002). Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks. Proceedings of the Fifteenth Conference of the International Organization of Citrus Virologists, 205-212. 2313-5123 http://hdl.handle.net/20.500.11939/7787 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/205-212%20IOCV15.pdf en 2001-11-11 Fifteenth Conference of the International Organization of Citrus Virologists Paphos, Cyprus This work was supported by INIA Projects SC93-110 and SC97-103. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Citrus leaf blotch virus Plant viruses PCR Rootstocks Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title | Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title_full | Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title_fullStr | Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title_full_unstemmed | Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title_short | Citrus Leaf Blotch Virus: A New Citrus Virus Associated with Bud Union Crease on Trifoliate Rootstocks |
| title_sort | citrus leaf blotch virus a new citrus virus associated with bud union crease on trifoliate rootstocks |
| topic | Citrus leaf blotch virus Plant viruses PCR Rootstocks |
| url | http://hdl.handle.net/20.500.11939/7787 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/205-212%20IOCV15.pdf |
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