Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis
A field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus and virus-like diseases by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an i...
| Autores principales: | , , , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7779 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th020_024.pdf |
| _version_ | 1855492358584401920 |
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| author | Moreno, Pedro Guerri, José Ballester-Olmos, José F. Martínez, M. E. |
| author2 | Brlansky, R. H. |
| author_browse | Ballester-Olmos, José F. Brlansky, R. H. Guerri, José Martínez, M. E. Moreno, Pedro |
| author_facet | Brlansky, R. H. Moreno, Pedro Guerri, José Ballester-Olmos, José F. Martínez, M. E. |
| author_sort | Moreno, Pedro |
| collection | ReDivia |
| description | A field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus and virus-like diseases by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an inconspicuous stem pitting on Mexican lime. Two months after aphid transmission, one of the infected lime seedlings was used as an inoculum source to graft-inoculate 40 citron plants. Two years later, the citron plants were analysed individually for dsRNA content and up to 23 different electrophoretic profiles were found. DsRNA analysis has been repeated three times through the year and the individual patterns remained unchanged. Thirteen of these CTV sub-isolates were
graft-transmitted to sweet orange seedlings and the corresponding dsRNA profiles were identical to those previously obtained from citron. All these subisolates induced mild symptoms of Mexican lime similar to those of the original isolate (T-385). These results substantiate that several CTV strains can infect a single citrus plant. |
| format | Objeto de conferencia |
| id | ReDivia7779 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77792025-04-25T14:53:40Z Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis Moreno, Pedro Guerri, José Ballester-Olmos, José F. Martínez, M. E. Brlansky, R. H. Silver stain H20 Plant diseases H10 Pests of plants U30 Research methods Symptoms Electrophoresis Citrus tristeza virus Strain differences A field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus and virus-like diseases by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an inconspicuous stem pitting on Mexican lime. Two months after aphid transmission, one of the infected lime seedlings was used as an inoculum source to graft-inoculate 40 citron plants. Two years later, the citron plants were analysed individually for dsRNA content and up to 23 different electrophoretic profiles were found. DsRNA analysis has been repeated three times through the year and the individual patterns remained unchanged. Thirteen of these CTV sub-isolates were graft-transmitted to sweet orange seedlings and the corresponding dsRNA profiles were identical to those previously obtained from citron. All these subisolates induced mild symptoms of Mexican lime similar to those of the original isolate (T-385). These results substantiate that several CTV strains can infect a single citrus plant. 2021-11-25T17:12:49Z 2021-11-25T17:12:49Z 1991 conferenceObject Moreno, P., Guerri, J., Ballester-Olmos, J. F. & Martinez, M. E. (1991). Segregation of citrus tristeza virus strains as evidenced by double stranded RNA (dsRNA) analysis. Proceedings of the Eleventh Conference of the International Organization of Citrus Virologists, 20-24. http://hdl.handle.net/20.500.11939/7779 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th020_024.pdf en 1989-11-06 Eleventh Conference of the International Organization of Citrus Virologists Orlando, Florida Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Silver stain H20 Plant diseases H10 Pests of plants U30 Research methods Symptoms Electrophoresis Citrus tristeza virus Strain differences Moreno, Pedro Guerri, José Ballester-Olmos, José F. Martínez, M. E. Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title | Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title_full | Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title_fullStr | Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title_full_unstemmed | Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title_short | Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis |
| title_sort | segregation of citrus tristeza virus strains evidenced by double stranded rna dsrna analysis |
| topic | Silver stain H20 Plant diseases H10 Pests of plants U30 Research methods Symptoms Electrophoresis Citrus tristeza virus Strain differences |
| url | http://hdl.handle.net/20.500.11939/7779 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/11th020_024.pdf |
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