Viroids in Gummy Bark Sources from the Sultanate of Oman
The gummy bark disease affects sweet oranges and rough lemon in some Mediterranean countries and most countries of the Near and Middle East regions. Diseased trees are usually stunted, and scraping the bark reveals gum deposits. Symptoms of gummy bark in sweet orange resemble those of cachexia on ma...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | conferenceObject |
| Language: | Inglés |
| Published: |
IOCV
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/7771 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/272-279%20IOCV%2016.pdf |
| _version_ | 1855032667413676032 |
|---|---|
| author | Bernad, Lucia Moreno, Pedro Bove, J. M. Durán-Vila, Núria |
| author2 | Hilf, M. E. |
| author_browse | Bernad, Lucia Bove, J. M. Durán-Vila, Núria Hilf, M. E. Moreno, Pedro |
| author_facet | Hilf, M. E. Bernad, Lucia Moreno, Pedro Bove, J. M. Durán-Vila, Núria |
| author_sort | Bernad, Lucia |
| collection | ReDivia |
| description | The gummy bark disease affects sweet oranges and rough lemon in some Mediterranean countries and most countries of the Near and Middle East regions. Diseased trees are usually stunted, and scraping the bark reveals gum deposits. Symptoms of gummy bark in sweet orange resemble those of cachexia on mandarin but the cachexia agent fails to induce symptoms in sweet orange. The causal agent of gummy bark is suspected to be a viroid, but previous studies conducted with sources from Turkey failed to identify a sequence variant of HSVd as the putative disease agent. In the present study, samples collected from Baladi, Valencia, Washington navel and Succari sweet orange trees showing the characteristic gummy bark symptoms were graftinoculated on Etrog citron and analyzed by sPAGE and slot-blot hybridization using viroid-specific probes. In addition to HSVd, all samples also contained CEVd, CVd-III and CVd-IV. Sequence analysis of DNA amplicons generated by RT-PCR using specific primers for these viroids identified novel variants of CEVd (CEVd-gb) and CVd-III (CVd-III-gb) in all the gummy sources, whereas CVd-IV was identical to previously reported sequences. The relationship of these viroids to the putative agent(s) of gummy bark is discussed. |
| format | conferenceObject |
| id | ReDivia7771 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77712025-04-25T14:53:16Z Viroids in Gummy Bark Sources from the Sultanate of Oman Bernad, Lucia Moreno, Pedro Bove, J. M. Durán-Vila, Núria Hilf, M. E. Exocortis Cachexia Citrus exocortis viroid Gummy bark disease HSVd CVd-III CVd-IV H20 Plant diseases Sweet oranges Viroids The gummy bark disease affects sweet oranges and rough lemon in some Mediterranean countries and most countries of the Near and Middle East regions. Diseased trees are usually stunted, and scraping the bark reveals gum deposits. Symptoms of gummy bark in sweet orange resemble those of cachexia on mandarin but the cachexia agent fails to induce symptoms in sweet orange. The causal agent of gummy bark is suspected to be a viroid, but previous studies conducted with sources from Turkey failed to identify a sequence variant of HSVd as the putative disease agent. In the present study, samples collected from Baladi, Valencia, Washington navel and Succari sweet orange trees showing the characteristic gummy bark symptoms were graftinoculated on Etrog citron and analyzed by sPAGE and slot-blot hybridization using viroid-specific probes. In addition to HSVd, all samples also contained CEVd, CVd-III and CVd-IV. Sequence analysis of DNA amplicons generated by RT-PCR using specific primers for these viroids identified novel variants of CEVd (CEVd-gb) and CVd-III (CVd-III-gb) in all the gummy sources, whereas CVd-IV was identical to previously reported sequences. The relationship of these viroids to the putative agent(s) of gummy bark is discussed. 2021-11-23T14:55:50Z 2021-11-23T14:55:50Z 2005 conferenceObject Bernad, L., Moreno, P., Bové, J. M. & Durán-Vila, N. (2005). Viroids in gummy bark sources from the Sultanate of Oman. Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 272-279. 2313-5123 0074-7203 (e-ISSN) http://hdl.handle.net/20.500.11939/7771 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/272-279%20IOCV%2016.pdf en 2004-11-07 Sixteenth Conference of the International Organization of Citrus Virologists Monterrey, Mexico This work was partially supported by RTA01-119 from the Ministerio de Ciencia y Tecnología (MCyT). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Exocortis Cachexia Citrus exocortis viroid Gummy bark disease HSVd CVd-III CVd-IV H20 Plant diseases Sweet oranges Viroids Bernad, Lucia Moreno, Pedro Bove, J. M. Durán-Vila, Núria Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title | Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title_full | Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title_fullStr | Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title_full_unstemmed | Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title_short | Viroids in Gummy Bark Sources from the Sultanate of Oman |
| title_sort | viroids in gummy bark sources from the sultanate of oman |
| topic | Exocortis Cachexia Citrus exocortis viroid Gummy bark disease HSVd CVd-III CVd-IV H20 Plant diseases Sweet oranges Viroids |
| url | http://hdl.handle.net/20.500.11939/7771 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/272-279%20IOCV%2016.pdf |
| work_keys_str_mv | AT bernadlucia viroidsingummybarksourcesfromthesultanateofoman AT morenopedro viroidsingummybarksourcesfromthesultanateofoman AT bovejm viroidsingummybarksourcesfromthesultanateofoman AT duranvilanuria viroidsingummybarksourcesfromthesultanateofoman |