Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage
Four citrus tristeza virus (CTV) isolates obtained by natural infection of Mexican lime seedlings kept in the field were graft-inoculated into Smooth Seville orange plants. Five years later, sweet orange and Mexican lime seedlings were graft-inoculated with the original isolates maintained in lime o...
| Autores principales: | , , , , , |
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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IOCV
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/7770 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/12th008_015.pdf |
| _version_ | 1855032667245903872 |
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| author | Moreno, Pedro Guerri, José Ballester-Olmos, José F. Fuertes-Polo, C. Albiach, Remedios Martínez, M. E. |
| author2 | Moreno, Pedro |
| author_browse | Albiach, Remedios Ballester-Olmos, José F. Fuertes-Polo, C. Guerri, José Martínez, M. E. Moreno, Pedro |
| author_facet | Moreno, Pedro Moreno, Pedro Guerri, José Ballester-Olmos, José F. Fuertes-Polo, C. Albiach, Remedios Martínez, M. E. |
| author_sort | Moreno, Pedro |
| collection | ReDivia |
| description | Four citrus tristeza virus (CTV) isolates obtained by natural infection of Mexican lime seedlings kept in the field were graft-inoculated into Smooth Seville orange plants. Five years later, sweet orange and Mexican lime seedlings were graft-inoculated with the original isolates maintained in lime or the sub-isolates kept in Smooth Seville orange. Variations in pathogenicity and/or
dsRNA profiles were observed among some ofthe isolates and the corresponding sub-isolates. Several sub-isolates, differing by pathogenicity and/or dsRNA profiles could be separated from the field isolate T- 385, which was obtained from a symptomless sweet/sour orange tree. One of these, sub-isolate T-317, induced mild symptoms in Mexican lime, and was symptomless in Etrog citron, rough lemon, sour orange and Eureka lemon, but induced vein clearing and stem pitting in sweet orange. The sub-isolate in sweet orange, T-318, was graft or aphid-transmitted to Mexican lime (sub-isolates T-305 and T-305a, respectively). These three sub-isolates induced severe vein clearing, vein corking, stem pitting and stunting in Mexican lime, vein clearing and stem pitting in citron, rough lemon and sweet orange, and seedling yellows in sour orange, Eureka lemon or Duncan grapefruit. T-317 and T-318 had similar dsRNA profiles, whereas those of T-305 and T-305* were different. When T-317 was graft-transmitted from citron to Mexican lime and then to sweet orange, the dsRNA profile of the new sub-isolate was similar to that of T-305, but the sweet orange plants were symptomless. The variations in pathogenicity and/or dsRNA observed indicate that many field isolates of CTV are mixtures of different strains, some of which can disappear or be at low titer when passed through certain hosts. |
| format | conferenceObject |
| id | ReDivia7770 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IOCV |
| publisherStr | IOCV |
| record_format | dspace |
| spelling | ReDivia77702025-04-25T14:53:12Z Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage Moreno, Pedro Guerri, José Ballester-Olmos, José F. Fuertes-Polo, C. Albiach, Remedios Martínez, M. E. Moreno, Pedro Aphid-transmission Graft-transmission Separation of strains Smooth Seville orange H20 Plant diseases Indicator plants Four citrus tristeza virus (CTV) isolates obtained by natural infection of Mexican lime seedlings kept in the field were graft-inoculated into Smooth Seville orange plants. Five years later, sweet orange and Mexican lime seedlings were graft-inoculated with the original isolates maintained in lime or the sub-isolates kept in Smooth Seville orange. Variations in pathogenicity and/or dsRNA profiles were observed among some ofthe isolates and the corresponding sub-isolates. Several sub-isolates, differing by pathogenicity and/or dsRNA profiles could be separated from the field isolate T- 385, which was obtained from a symptomless sweet/sour orange tree. One of these, sub-isolate T-317, induced mild symptoms in Mexican lime, and was symptomless in Etrog citron, rough lemon, sour orange and Eureka lemon, but induced vein clearing and stem pitting in sweet orange. The sub-isolate in sweet orange, T-318, was graft or aphid-transmitted to Mexican lime (sub-isolates T-305 and T-305a, respectively). These three sub-isolates induced severe vein clearing, vein corking, stem pitting and stunting in Mexican lime, vein clearing and stem pitting in citron, rough lemon and sweet orange, and seedling yellows in sour orange, Eureka lemon or Duncan grapefruit. T-317 and T-318 had similar dsRNA profiles, whereas those of T-305 and T-305* were different. When T-317 was graft-transmitted from citron to Mexican lime and then to sweet orange, the dsRNA profile of the new sub-isolate was similar to that of T-305, but the sweet orange plants were symptomless. The variations in pathogenicity and/or dsRNA observed indicate that many field isolates of CTV are mixtures of different strains, some of which can disappear or be at low titer when passed through certain hosts. 2021-11-23T14:55:34Z 2021-11-23T14:55:34Z 1993 conferenceObject Moreno, A., Guerri, J., Ballester-Olmos, J. F., Fuertes-Polo, C., Albiach, R. & Martinez, M. (1993). Variations of Pathogenicity and Double-stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolated Induced by Host Passage. Proceedings of the twelfth International Organization of Citrus Virologists Conference, 8-15. http://hdl.handle.net/20.500.11939/7770 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/12th008_015.pdf en 1992-11-23 Twelfth Conference of the International Organization of Citrus Virologists New Delhi, India This work was supported by the Spain-USA Committee for Scientific and Technical Cooperation (Grant no. 156) and by the Instituto Nacional de Investigaciones Agrarias (INIA) (Project no. 8179). Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess IOCV electronico |
| spellingShingle | Aphid-transmission Graft-transmission Separation of strains Smooth Seville orange H20 Plant diseases Indicator plants Moreno, Pedro Guerri, José Ballester-Olmos, José F. Fuertes-Polo, C. Albiach, Remedios Martínez, M. E. Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title | Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title_full | Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title_fullStr | Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title_full_unstemmed | Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title_short | Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage |
| title_sort | variations in pathogenicity and double stranded rna dsrna patterns of citrus tristeza virus isolate induced by host passage |
| topic | Aphid-transmission Graft-transmission Separation of strains Smooth Seville orange H20 Plant diseases Indicator plants |
| url | http://hdl.handle.net/20.500.11939/7770 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/12th008_015.pdf |
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